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| 1 | Immunohistochemical expression of SP-NK-1R-EGFR pathway and VDR in colonic inflammation and neoplasia显示文摘AIM:To determine the expression of neurokinin-1receptor(NK-1R),phosphorylated epidermal growth factor receptor(p EGFR),cyclooxygenase-2(Cox-2),and vitamin D receptor(VDR)in normal,inflammatory bowel disease(IBD),and colorectal neoplasia tissues from Puerto Ricans.METHODS:Tissues from patients with IBD,colitisassociated colorectal cancer(CAC),sporadic dysplasia,and sporadic colorectal cancer(CRC),as well as normal controls,were identified at several centers in Puerto Rico.Archival formalin-fixed,paraffin-embedded tissues were de-identified and processed by immunohistochemistry for NK-1R,p EGFR,Cox-2,and VDR.Pictures of representative areas of each tissues diagnosis were taken and scored by three observers using a4-point scale that assessed intensity of staining.Tissues with CAC were further analyzed by photographing representative areas of IBD and the different grades of dysplasia,in addition to the areas of cancer,within each tissue.Differences in the average age between the five patient groups were assessed with one-way analysis of variance and Tukey-Kramer multiple comparisons test.The mean scores for normal tissues and tissues with IBD,dysplasia,CRC,and CAC were calculatedand statistically compared using one-way analysis of variance and Dunnett’s multiple comparisons test.Correlations between protein expression patterns were analyzed with the Pearson’s product-moment correlation coefficient.Data are presented as mean±SE.RESULTS:On average,patients with IBD were younger(34.60±5.81)than normal(63.20±6.13,P<0.01),sporadic dysplasia(68.80±4.42,P<0.01),sporadic cancer(74.80±4.91,P<0.001),and CAC(57.50±5.11,P<0.05)patients.NK-1R in cancer tissue(sporadic CRC,1.73±0.34;CAC,1.57±0.53)and sporadic dysplasia(2.00±0.45)were higher than in normal tissues(0.73±0.19).p EGFR was significantly increased in sporadic CRC(1.53±0.43)and CAC(2.25±0.47)when compared to normal tissue(0.07±0.25,P<0.05,P<0.001,respectively).Cox-2 was significantly increased in sporadic colorectal cancer(2.20±0.23 vs 0.80±0.37 for normal tissues,P<0.05).In comparison to normal(2.80±0.13)and CAC(2.50±0.33)tissues,VDR was significantly decreased in sporadic dysplasia(0.00±0.00,P<0.001 vs normal,P<0.001 vs CAC)and sporadic CRC(0.47±0.23,P<0.001 vs normal,P<0.001 vs CAC).VDR levels negatively correlated with NK-1R(r=-0.48)and p EGFR(r=-0.56)in normal,IBD,sporadic dysplasia and sporadic CRC tissue,but not in CAC.CONCLUSION:Immunohistochemical NK-1R and p EGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia.VDR immunoreactivity can distinguish CAC from sporadic cancer. | Raymond A Isidro Myrella L Cruz Angel A Isidro Axel Baez Axel Arroyo William A González-Marqués Carmen González-Keelan Esther A Torres Caroline B Appleyard | 2015 | World Journal of Gastroenterology2015，21，6： | 7 |
| 2 | Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease显示文摘AIM: To determine serum vitamin D levels and colonic vitamin D receptor(VDR) expression in inflammatory bowel disease(IBD) and non-IBD patients and correlate these with histopathology.METHODS: Puerto Rican IBD(n = 10) and non-IBD(n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis(active/inactive), and scored for the degree of inflammation present(0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.RESULTS: The IBD cohort was significantly younger(40.40 ± 5.27, P < 0.05) than the non-IBD cohort(56.70 ± 1.64) with a higher prevalence of vitamin D deficiency(40% vs 20%, respectively) and insufficiency(70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients(1.95 ± 0.25) than in normalappearing mucosa from control patients(0.25 ± 0.08, P < 0.01) and from IBD patients(0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients(r =-0.44, P < 0.05) and from IBD patients with Crohn's disease(r =-0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients(r = 0.38, P < 0.05) and with patient age(r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. | Yamilka Abreu-Delgado Raymond A Isidro Esther A Torres Alexandra González Myrella L Cruz Angel A Isidro Carmen I González-Keelan Priscilla Medero Caroline B Appleyard | 2016 | World Journal of Gastroenterology2016，22，13： | 5 |
| 3 | Hepatitis C eradication with sofosbuvir leads to significant metabolic changes显示文摘AIM To assess the effect of sofosbuvir(SOF) based regimens on glycemic and lipid control.METHODS This is a retrospective analysis of hepatitis C virus(HCV)-infected patients treated and cured with a SOF regimen [SOF/ribavirin/interferon, SOF/simeprevir, or SOF/ledipasvir(LDV) ± ribavirin] from January 2014 to March 2015. Patients with hemoglobin A1C(HbA1C) and lipid panels within six months before and six months after therapy were identified and included in our study. Due to the known hemolytic effect of ribavirin, HbA1C was obtained a minimum of three months post-treatment for the patients treated with a ribavirin regimen. Medical history, demographics, HCV genotype, pre-therapy RNA, and liver biopsies were included in our analysis. The patients who started a new medication or had an adjustment of baseline medical management for hyper-lipidemia or diabetes mellitus(DM) were excluded from our analysis.RESULTS Two hundred and thirty-four patients were reviewed, of which 60 patients met inclusion criteria. Sixty-three point three percent were male, 26.7% were Caucasian, 41.7% were African American and 91.7% were infected with hepatitis C genotype 1. Mean age was 60.6 ± 6.7 years. Thirty-nine patients had HbA1C checked before and after treatment, of which 22 had the diagnosis of DM type 2. HbA1C significantly decreased with treatment of HCV(pretreatment 6.66% ± 0.95% vs posttreatment 6.14% ± 0.65%, P < 0.005). Those treated with SOF/LDV had a lower HbA1C response than those treated with other regimens(0.26% ± 0.53% vs 0.71% ± 0.83%, P = 0.070). Fifty-two patients had pre- and post-treatment lipid panels; there was a significant increase in low-density lipoprotein(LDL) and total cholesterol(TC) after treatment(LDL: 99.5 ± 28.9 mg/dL vs 128.3 ± 34.9 mg/dL, P < 0.001; TC: 171.6 ± 32.5 mg/dL vs 199.7 ± 40.0 mg/dL, P < 0.001). Pretreatment body-mass index(BMI) did not differ from post-treatment BMI(P = 0.684). CONCLUSION Eradication of HCV with a SOF regimen resulted in a significant drop in HbA1C and an increase in LDL and TC post therapy. | Amilcar L Morales Zachary Junga Manish B Singla Maria Sjogren Dawn Torres | 2016 | World Journal of Hepatology2016，8，35： | 2 |
| 4 | Complete FDTD analysis of microwave heating processes in frequency-dependent and temperature-dependent Media显示文摘 | Torres F Jecko B | 1997 | IEEE Trans on Microwave Theory and Techniques1997，45，1： | 2 |
| 5 | Carbon material and bioenergetic balances of xylitol production from corncobs by Debaryomyces hansenii 显示文摘 | RIVAS B TORRE P DOMINGUEZ JM | 2003 | Bioteehnol Prog2003，19，3： | 1 |
| 6 | Incidence,risks and prognosis of nosocomial pneumonia in mechanically ventilated patients显示文摘 | Torres A Aznar B Gatell J M | 1990 | Am Rev Respir Dis1990，142，： | 1 |
| 7 | Association of the CT60 mark- er of the CTLA4 gene with systemic lupus erythematosus显示文摘 | Torres B Aguilar F Franco E | 2004 | Arthri- tis & Rheumatism2004，50，7： | 1 |
| 8 | Peptide vaccine candidates against classical swine fever virus: T cell and neutrali- zing antibody responses of dendrimers displaying E2 and NS2-3 epitopes显示文摘 | Monso M Tarradas J de la Torre B G | 2011 | J Pept Sci2011，17，1： | 1 |
| 9 | Kinetics of consumption of fermentation products by anode-respiring bacteria显示文摘 | Torres C I Kato Marcus A Rittmann B E | 2007 | Appl Microbiol Biotechnol2007，77，3： | 1 |
| 10 | Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea 显示文摘 | WALKER C B DE LA TORRE J R KLOTZ M G | 2010 | Proceedings of the National Academy of Sciences of USA2010，107，： | 1 |
| 11 | Biomarkers and community-acquired pneumonia : tailoring management with biological data显示文摘 | TORRES A RAMIREZ P MONTULL B | 2012 | Semin Respir Crit Care Med2012，33，3： | 1 |
| 12 | Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine 显示文摘 | Garcia B Neninger E Torre A | 2008 | Clin Cancer Res2008，14，3： | 1 |
| 13 | Genetic relations- hips among Orobanche pecies as revealed by PAPD analysis显示文摘 | Roman B Alfaro C Torres AM | 2003 | Ann Bot(Lond)2003，91，6： | 1 |
| 14 | E-Gover- nance Developments in European Union Cities: Reshaping Government' s Rela- tionship with Citizens显示文摘 | Torres L Pina V Acerete B | 2006 | Governance2006，，2： | 1 |
| 15 | Borna disease virus (BDV),a non- segmented RNA virus, replicates in the nuclei of infected cells where infectious BDV ribonucleoproteins are present 显示文摘 | Cubitt B De La Torre JC | 1994 | J Virol1994，68，3： | 1 |
| 16 | Bacteria-host com- munication: the language of hormones显示文摘 | Sperandio V Torres AG Jarvis B | 2003 | Proc Natl Acad Sci U S A2003，100，15： | 1 |
| 17 | Combination of Phthalocyanine and Fullerene Moieties for Optical Limiting显示文摘 | Doyle J J Ballesteros B de la Torre G | 2006 | Chem Phys Lett2006，428，4： | 1 |
| 18 | Blood outgrowth endothelial cells reduce hypoxia-mediated fi- broblast to myofibroblast conversion by decreasing proan- giogenic cytokines显示文摘 | Nieves Torres EC Yang B Brahmbhatt A | 2014 | J Vasc Res2014，51，6： | 1 |
| 19 | Analysis of the strain transfer in a new FBG sensor for structural health monitoring显示文摘 | Torres B Payá-Zaforteza I Calderón P A | 2011 | Engineering Structures2011，33，2： | 1 |
| 20 | Conduction-based modeling of the biofilm anode of a microbial fuel cell显示文摘 | KATO MARCUS A TORRES C I RITTMANN B E | 2007 | Biotechnology and Bioengeering2007，98，6： | 1 |
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