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| 1 | Hepatitis B virus genotypes in southwest Iran: Molecular, serological and clinical outcomes显示文摘AIM: To investigate the associations of hepatitis B virus (HBV) genotype with HBeAg and anti-HBe status, alanine aminotransferase (ALT) levels and HBV-DNA detection in different groups of HBV-infected patients in southwest Iran. METHODS: A total of 89 HBsAg-positive serum samples were collected from the same number of patients. All sera were then investigated to determine HBV DNA and serological markers. For all the polymerase chain reaction (PCR)-positive samples, biochemical, histopathological assays and genotyping were also performed. RESULTS: Genotype D was the only type of HBV foundin different clinical forms of acute and chronic infections. There was a high prevalence of HBeAg-negative HBV- infected patients with chronic hepatitis (52.7%). Out of 55 patients with chronic hepatitis, seven (12.7%) were diagnosed with cirrhosis. A significant association between the presence of anti-HBe antibody and an increase in ALT level, among either HBeAg-negative (P = 0.01) or HBeAg-positive (P = 0.026) patients, was demonstrated. No significant differences were observed between the clinical outcomes of HBeAg-positive and -negative individuals (P = 0.24). CONCLUSION: Genotype D has been recognized as the only type of HBV found in different clinical forms of HBV infections, including cirrhosis, among the residents of southwest Iran. Anti-HBe possibly plays a role in disease progression in some patients with chronic hepatitis, at least for a period of disease. | Anahita Mojiri Abbas Behzad-Behbahani Mehdei Saberifirozi Maryam Ardabili Mahmood Beheshti Marjan Rahsaz Mehrdad Banihashemi Negar Azarpira Bita Geramizadeh Baharak Khadang Afsaneh Moaddeb Mojgan Ghaedi Tahereh Heidari Ardeshir Torab Alireza Salah Saeid Amirzadeh Zahra Jowkar Davood Mehrabani Samad Amini-Bavil-Olyaee Mohammad Ali Dehyadegari | 2008 | World Journal of Gastroenterology2008，14，10： | 4 |
| 2 | In vitro differentiation of human umbilical cord Wharton's jelly mesenchymal stromal cells to insulin producing clusters显示文摘AIM: To investigate the differentiation of human Wharton's jelly derived mesenchymal stromal cells(WJMSCs) to insulin producing clusters(IPC) this study was conducted. METHODS: The umbilical cords samples were collected from full term caesarian section mothers and the WJ-MSCS were cultured from tissue explants in High glucose-Dulbecco's Modified Eagle Medium(H-DMEM); H-DMEM supplemented with 10% fetal bovine serum(FBS) and antibiotics. The expression of CD90, CD44, CD105, CD34 and CD133 as well as osteogenic and adipogenic differentiation of cells in appropriate medium were also evaluated. The cells were differentiated toward IPC with changing the culture medium and adding the small molecules such as nicotinic acid, epidermal growth factor, and exendin-4 during 3 wk period. The gene expression of PDX1, NGN3, Glut2, insulin was monitored by reveres transcription polymerase chain reaction method. The differentiated clusters were stained with Dithizone(DTZ) which confirms the presence of insulin granules. The insulin challenge test(low and high glucose concentration in Krebs-Ringer HEPES buffer) was also used to evaluate the functional properties of differentiated clusters.RESULTS: WJ-MSCS were positive for mesenchymal surface markers(CD90, CD44, CD105), and negative for CD34 and CD133. The accumulation of lipid vacuoles and deposition of calcium mineral in cells were considered as adipogenic and osteogenic potential of WJ-MSCS. The cells also expressed the transcriptional factors such as Nanog and OCT4. During this three step differentiation, the WJ-MSCS morphology was gradually changed from spindle shaped cells in to epithelioid cells and eventually to three dimensional clusters. The clusters expressed PDX1, NGN3, Glut2, and insulin. The cells became bright red color when stained with DTZ and the insulin secretion was also confirmed. In glucose challenge test a significant increase in insulin secretion from 0.91 ± 0.04 μIu/mL(2.8 mmol/L glucose) to to 8.34 ± 0.45 μIu/mL(16.7 mmol/L glucose) was recorded(P < 0.05). The insulin secretion of undifferentiated WJ-MSCS was not changed in this challenge test.CONCLUSION: WJ-MSCs have the ability to differentiate in to islet-like cells in vitro. However, this process needs further optimization in order to generate efficient and functional IPCs. | Seideh Masoomeh Nekoei Negar Azarpira Ladan Sadeghi Sulmaz Kamalifar | 2015 | World Journal of Clinical Cases2015，3，7： | 3 |
| 3 | Metformin alleviates cholestasis-associated nephropathy through regulating oxidative stress and mitochondrial function显示文摘Background and aim:Cholestasis-associated renal injury or cholemic nephropathy(CN)is a serious clinical problem.Previous studies mentioned that oxidative stress and mitochondrial impairment play a role in CN.There is no specific pharmacological intervention for CN.Metformin is an anti-diabetic drug administered for decades.On the other hand,novel pharmacological properties have emerged for this drug.The effect of metformin on oxidative stress parameters has been well-recognized in different experimental models.It has also been found that metformin positively affected mitochondrial function.The current study aimed to evaluate the effects of metformin in an animal model of CN.Methods:Rats underwent bile duct ligation(BDL)and were treated with metformin(250 and 500 mg/kg)for 14 consecutive days.Two weeks after the BDL operations,urine,serum,and kidney samples were collected and analyzed.Results:Markers of oxidative stress,including reactive oxygen species(ROS)formation,lipid peroxida-tion,protein carbonylation,depleted antioxidant capacity,and decreased glutathione(GSH)levels were detected in BDL animals.Moreover,mitochondrial indices,including adenosine triphosphate(ATP)level,dehydrogenase activity,mitochondrial membrane potential,and mitochondrial permeability,were impaired in the kidney of cholestatic rats.Renal histopathological alterations in cholestatic animals included tubular degeneration and interstitial inflammation,cast formation,and fibrosis.It was found that metformin significantly alleviated oxidative stress and improved mitochondrial indices in the kidney of cholestatic rats.Tissue histopathological alterations were also mitigated in metformin-treated groups.Conclusions:Metformin could be a candidate for managing CN.The nephroprotective role of metformin is primarily associated with its effects on oxidative stress parameters and mitochondrial function. | Mohammad Mehdi Ommati Hamidreza Mohammadi Khadijeh Mousavi Negar Azarpira Omid Farshad Reyhaneh Dehghani Asma Najibi Sedigheh Kamran Hossein Niknahad Reza Heidari | 2021 | Liver Research2021，5，3： | 3 |
| 4 | Factors associated with recurrence of clubfoot treated by the Ponseti method显示文摘AIM To assess several associated factors on the recurrence of clubfoot after successful correction by the Ponseti method. METHODS A total of 115 children with 196 clubfeet deformities, treated by the Ponseti method, were evaluated. Demographic data, family history of clubfoot in firstdegree relatives, maternal educational level and brace compliance were enquired. Based on their medical files, the characteristics of the patients at the time of presentation such as age, possible associated neuromuscular disease or especial syndrome, severity of the deformity according to the Dimeglio grade and Pirani score, residual deformity after previous Ponseti method and number of casts needed for the correction were recorded.RESULTS There were 83 boys(72.2%) and 32 girls(27.8%) with a male to female ratio of 2.6. The mean age at the initiation of treatment was 5.4 d(range: 1 to 60 d). The average number of casts applied to achieve complete correction of all clubfoot deformities was 4.2. Follow-up range was 11 to 60 mo. In total, 39 feet had recurrence with a minimum Dimeglio grade of 1 or Pirani score of 0.5 at the follow-up visit. More recurrence was observed in non-idiopathic clubfoot deformities(P = 0.001), noncompliance to wear braces(P < 0.001), low educational level of mother(P = 0.033), increased number of casts(P < 0.001), and more follow-up periods(P < 0.001). No increase in the possibility of recurrence was observed when the previous unsuccessful casting was further treated using the Ponseti method(P = 0.091). Also, no significant correlation was found for variables of age(P = 0.763), Dimeglio grade(P = 0.875), and Pirani score(P = 0.624) obtaining at the beginning of the serial casting. CONCLUSION Using the Ponseti method, non-idiopathic clubfoot, noncompliance to wear braces, low educational level of mother, increased number of casts and more followup periods had more association to possible increase in recurrence rate after correction of clubfoot deformity. | Mohammad Reza Azarpira Mohammad Jafar Emami Amir Reza Vosoughi Keivan Rahbari | 2016 | World Journal of Clinical Cases2016，4，10： | 2 |
| 5 | Mitigation of cholestasis-associated hepatic and renal injury by edaravone treatment: Evaluation of its effects on oxidative stress and mitochondrial function显示文摘Background and aim:The liver is the primary organ affected by cholestasis,and complications such as renal injury,renal failure,and the need for renal transplantation are associated with cholestatic liver disease.There is substantial evidence indicating that reactive oxygen species(ROS)and mitochondrial impairment are fundamental mechanisms underlying cholestasis-induced hepatic and renal injury.Edaravone(EDV)is a potent radical scavenger and antioxidant that may prevent oxidative stress and improve impaired mitochondrial function in various diseases.This study was performed to evaluate the effects and mechanisms of action of EDV on hepatic and renal injury in an animal model of cholestasis.Methods:Rats subjected to bile duct ligation(BDL)were treated with EDV 1 or 10 mg/kg/day intraper-itoneally for 14 consecutive days.Biomarkers of oxidative stress and mitochondrial impairment in the liver and kidney were assessed in EDV-treated and untreated rats with cholestasis.Results:Significant increases in tissue ROS level,lipid peroxidation,protein carbonylation,and oxidized glutathione level were detected in rats subjected to BDL.Additionally,significant decreases in tissue glutathione level and antioxidant capacity were observed in the hepatic and renal tissues of rats with cholestasis.Markers of mitochondrial impairment,including mitochondrial depolarization,lipid perox-idation,mitochondrial permeabilization,depleted adenosine triphosphate content,and decreased de-hydrogenase activity,were also detected in rats subjected to BDL.Furthermore,portal inflammation,necrosis,and tissue fibrosis were detected in the liver and significant tubular atrophy and interstitial inflammation,as well as fibrotic lesions,were detected in the kidneys of rats with cholestasis.EDV treatment significantly mitigated cholestasis-associated hepatic and renal injury.Conclusions:The antioxidative properties of EDV and its positive effects on the indices of mitochondrial function may be critical factors contributing to protection against cholestasis-associated hepatic and renal injury. | Mohammad Mehdi Ommati Hanie Attari Asma Siavashpour Marzieh Shafaghat Negar Azarpira Hasti Ghaffari Leila Moezi Reza Heidari | 2021 | Liver Research2021，5，3： | 2 |
| 6 | Immunomodulatory effects of mice mesenchymal stem cells on maturation and activation of dendritic cells显示文摘 | Sadeghi L Kamali-Sarvestani E Azarpira N | 2014 | Iran J Immunol2014，11，3： | 1 |
| 7 | Plasma Cell Tumors with Neurologic Symptoms:Cytological Findings显示文摘 | Azarpira N Vasei M Rasekhi A | | 0，，03： | 1 |
| 8 | Polymorph-ism of adiponectin (45T/G) and adiponectin receptor-2 (795G/A) in an Iranian population:relation with insulin' resistance and response to treatment with pioglitaz0ne in patients with type 2 diabetes mellitus显示文摘 | Namvaran F Rahimi-Moghaddam P Azarpira N | 2012 | Molecular Biology Reports2012，，5： | 1 |
| 9 | Extramedullary plasmacytoma of the nasal cavity report of three cases with review of the literature 显示文摘 | Ashraf M J Azarpira N Khademi B | 2013 | lran Red Crescent Med2013，15，4： | 1 |
| 10 | Effects of tomato extract on oxidative stress induced toxicity in different organs of rats 显示文摘 | Jamshidzadeh A Baghban M Azarpira N | 2008 | Food Chem Toxicol2008，46，12： | 1 |
| 11 | Angiotensinogen,angiotensine converting enzyme andplasminogen activator inhibitor-1 gene polymorphism in chronic al-lograft dysfunction显示文摘 | Azarpira N Bagheri M Raisjalali G A Aghdaie M H Behzadi S Salahi H | 2009 | Mol Biol Rep2009，36，： | 1 |
| 12 | Extramedullary plasmacy- toma of the nasal cavity report of three cases with review of the lit- erature显示文摘 | Ashraf MJ Azarpira N Khademi B | 2013 | Iran Red Crescent MedJ2013，15，4： | 1 |
| 13 | Investigation of allele and genotype frequencies of CYP2C9, CYP2C19 and VKORC1 in Iran 显示文摘 | Azarpira N Namazi S Hendijani F | 2010 | Pharmacol Rep2010，62，： | 1 |
| 14 | Reactions of dehydrodiferulates with ammonia显示文摘 | Azarpira A Lu F C Ralpha J | 2011 | Organic & Biomolecular Chemistry2011，9，19： | 1 |
| 15 | Extramedullary plasmacytoma of the nasal cavity report of three cases with re- view of the literature显示文摘 | Ashraf MJ Azarpira N Khademi B | 2013 | Iran Red Crescent Med J2013，15，4： | 1 |
| 16 | Prevention of arthrofibrosis by monoclonal antibody against vascular endothelial growth factor: a novel use of bevacizumab in rabbits 显示文摘 | Emami M J Jaberi FM Azarpira N | 2012 | Orthop Traumatol Surg Res2012，98，7： | 1 |
| 17 | SLC3OA8 gene polymorphism (rs13266634 Cf显示文摘 | Faghih H Khatami SR Azarpira N | 2014 | Mol Biol Rep2014，41，5： | 1 |
| 18 | Investigaion of allele and genotype frequencies of CYP2C9, CYP2CI9 and VKORC1 in Iran显示文摘 | AZARPIRA N NAMAZI S HENDIJANI F | 2010 | Pharmacol Rep2010，62，4： | 1 |
| 19 | Superioroblique tendon expansion with Achilles tendon allograft fortreating Brown syndrome显示文摘 | Talebnejad MR Mosallaei M Azarpira N | 2011 | J AAPOS2011，15，3： | 1 |
| 20 | The small round blue cell tumors of the sinonasal area:histological and immunohistochemical findings显示文摘 | J Ashraf M Beigomi L Azarpira N | 2013 | Iran Red Crescent Med J2013，15，6： | 1 |
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