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| 1 | Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization显示文摘Background:Heavy metal cadmium(Cd)is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and fertility.It has been reported that Cd exposure impaired the quality of oocytes and led to a defective maturation and fertilization,through oxidative stress induction.Resveratrol(Res)is a natural polyphenol with strong antioxidant properties that exhibited protective role in preventing oocyte redox homeostasis disruption and quality decline.Here,we explored whether the addition of Res to in vitro maturation(IVM)medium might act as a protection against Cd-induced toxicity on ovine oocyte maturation and fertilization.Firstly,we evaluated the effect of supplementing IVM medium with two different Res concentrations(1and 2μmol/L)on nuclear maturation and fertilization of oocytes matured under CdCl2(2μmol/L)exposure.Therefore,the concentration of 1μmol/L Res was selected to analyse the effects of this compound on intracellular ROS levels,mitochondrial(mt)distribution and activity,chromatin configuration,cytoskeleton morphology,cortical granules(CGs)distribution and mRNA expression of genes associated with cellular response to oxidative stress(i.e.SIRT1,SOD 1,GPX1,GSR,CAT)in Cd-exposed in vitro matured oocytes.Results:We found that 1μmol/L Res restored the reduced oocyte meiotic competence induced by Cd exposure as well as,Res sustained oocyte ability to be normally fertilized and decreased polyspermic fertilization at both tested concentrations.Moreover,we demonstrated that 1μmol/L Res mitigated Cd-induced alterations of oocyte cytoplasmic maturation by reducing reactive oxygen species(ROS)accumulation,preventing mt dysfunction,maintaining the correct meiotic spindle and cortical F-actin assembly and the normal cortical granule distribution as well as up-regulating SIRT1,SOD1 and GPX1 genes.Conclusions:Taken together,our findings highlighted the beneficial influence exerted by Res in preventing Cdinduced disturbance of nuclear and cytoplasmic maturation and subsequent fertilization in ovine oocytes.Res treatment may help to establish defence strategies counteracting Cd-induced toxicity on the female gamete. | Anna Rita Piras Federica Ariu Alessio Maltana Giovanni Giuseppe Leoni Nicola Antonio Martino Antonella Mastrorocco Maria Elena Dell’Aquila Luisa Bogliolo | 2023 | Journal of Animal Science and Biotechnology2023，14，1： | 3 |
| 2 | PAI-1 promotes extracellular matrix deposition in the airways of a murine asthma model 显示文摘 | Oh CK Ariue B Alban RF | 2002 | Biochem Biophys Res Commun2002，294，5： | 1 |
| 3 | Expression pattern of zygote arrest 1 (ZAR1), maternal antigen that embryo requires (MATER), growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) genes in ovine oocytes and in vitro- produced preimplantation embryos显示文摘 | Bebbere D Bogliolo L Ariu F | 2008 | Reprod Fertil Dev2008，20，： | 1 |
| 4 | Understanding the origin of the 535 nm emission band in oxidized poly(9,9- dioctylfluorene) : The essential role of inter-chain/inter-segment interactions显示文摘 | Sims M Bradley D D C Ariu M | 2004 | Adv Func Mater2004，14，8： | 1 |
| 5 | Morphological and biochemical analysis of immature ovine oocytes vitrified with or without cumulus cells显示文摘 | Ariu F Fois S | 2007 | Theriogenology2007，68，8： | 1 |
| 6 | PAI 1 promotes extra ccllular matrix deposition in the airways of a routine asthma model显示文摘 | Oh CK Ariue B Alban RF | 2002 | Biochem Biophys Res Commun2002，，294： | 1 |
| 7 | Exciton migration in β-phase poly (9, 9-dioctylfluorene) 显示文摘 | Ariu M Sims M Rahn M D | 2003 | Phys Rev2003，67，19： | 1 |
| 8 | McPAD:A multiple classifier system for accurate payload-based anomaly detection显示文摘 | R Perdisci D Ariu P Fogla | 2009 | Computer Networks(S1389-1286)2009，53，6： | 1 |
| 9 | Vitrification of immture and in vitro matured horse oocytes显示文摘 | Bogliolo L Ariu F Rosati I | 2006 | Reprod Fertil2006，18，： | 1 |
| 10 | Morphological and biochemical a- nalysis of immature ovine oocytes vitrified with or without cumulus cells 显示文摘 | Bogliolo L Ariu F Fois S | 2007 | Theriogenology2007，68，8： | 1 |
| 11 | Understanding the origin of the 535 nm emission band in oxidized poly(9,9-dioctylfluorene) :The essential role of inter-chain/inter-segment interactions 显示文摘 | SIMS M BRADLEY D D C ARIU M | 2004 | Adv Funct Mater2004，14，8： | 1 |
| 12 | Expression pattern of zygote arrest 1(ZAR1), maternal antigen that embryo requires (MA-TER) , growth differentiation factor 9 (GDF9)and bone morphogenetie protein 15 (BMP-15)genes in ovine oocytes and in vitro-produced preimplant- ation embryos 显示文摘 | Bebbere D Bngliolo L Ariu F | 2008 | Reprod Fertil Dev2008，20，8： | 1 |
| 13 | PAI-1 promotes extracellular matrix deposition in the airways of a murine asthma model显示文摘 | Oh CK Ariue B Alban RF | 2002 | Biochem Biophys Res Commun2002，294，5： | 1 |
| 14 | Embryos produced in vitro from prepubertal lamb and adult sheep oocytes display different gene expression patterns显示文摘 | Bebbere D Bogliolo L Ariu F | 2009 | Reprod Fertil Develop2009，21，1： | 1 |
| 15 | Expression pattern of zygote arrest 1 ( ZAR1 ), maternal antigen that embryo requires ( MATER) ,growth differentiation factor 9 ( GDF9 ) and bone morphogenetie protein 15 (BMP15)genes in oviue ooeytes and in vitro-produced preimplantation embryos 显示文摘 | Bebbere D Bogliolo L Ariu F | 2008 | Reprod Fertil Dev2008，20，8： | 1 |
| 16 | PAI-1 promotes extracellular matrix deposition in the airways of a routine asthma model 显示文摘 | Oh C K Ariue B Alban R F | 2002 | Biochem Biophys Res Commun2002，294，： | 1 |
| 17 | PAI-1 promotes extracellulax matrix deposition in the airways of a murine astlmla model显示文摘 | Oh C K Ariue B Alban R F | 2002 | Biochem biophys res commun2002，294，： | 1 |
| 18 | MCPAd--a multiple classifier system for accurate payload-based anomaly detec- tion显示文摘 | Perdisci R Ariu D Fogla P | 2009 | Computer Networks2009，53，6： | 1 |
| 19 | Scalable fine-grained behavioral clustering of HTTP-based malware显示文摘 | Roberto Perdisci Davide Ariu Giorgio Giacinto | 2012 | Computer Networks2012，，： | 1 |
| 20 | PAI-1 promotes extracellular matrix deposition in the airways of a murine asthma model显示文摘 | Oh CK Ariue B Alban RF | 2002 | Biochem Biophys Res Commun2002，294，5： | 1 |
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