1篇
您的检索式：作者名="Anna Mattout"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Global epigenetic changes during somatic cell reprogramming to iPS cells显示文摘Embryonic stem cells(ESCs)exhibit unique chromatin features,including a permissive transcriptional program and an open,decondensed chromatin state.Induced pluripotent stem cells(iPSCs),which are very similar to ESCs,hold great promise for therapy and basic research.However,the mechanisms by which reprogramming occurs and the chromatin organization that underlies the reprogramming process are largely unknown.Here we characterize and compare the epigenetic landscapes of partially and fully reprogrammed iPSCs to mouse embryonic fibroblasts(MEFs)and ESCs,which serves as a standard for pluripotency.Using immuno-fluorescence and biochemical fractionations,we analyzed the levels and distribution of a battery of histone modifications(H3ac,H4ac,H4K5ac,H3K9ac,H3K27ac,H3K4me3,H3K36me2,H3K9me3,H3K27me3,and gH2AX),as well as HP1a and lamin A.We find that fully reprogrammed iPSCs are epigenetically identical to ESCs,and that partially reprogrammed iPSCs are closer to MEFs.Intriguingly,combining both time-course reprogramming experiments and data from the partially reprogrammed iPSCs,we find that heterochromatin reorganization precedes Nanog expression and active histone marking.Together,these data delineate the global epigenetic state of iPSCs in conjunction with their pluripotent state,and demonstrate that heterochromatin precedes euchromatin in reorganization during reprogramming. | Anna Mattout Alva Biran Eran Meshorer | 2011 | Journal of Molecular Cell Biology2011，3，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员