| 1 | Polymerase chain reaction: A sensitive method for detecting Helicobacter pyloriinfection in bleeding peptic ulcers显示文摘AIM: To assess the sensitivity and specificity of polymerase chain reaction (PCR) in detecting Helicobacter pylori(H pylori)infection in patients with bleeding peptic ulcers, and to compare its diagnostic efficacy with other invasive and non-invasive tests.METHODS: From April to September 2002, H pylori status in 60 patients who consecutively presented with gastroduodenal ulcer bleeding was examined by rapid urease tests (RUT), histology, culture, PCR, serology and urea breath tests (UBT).RESULTS: The sensitivity of PCR was significantly higher than that of RUT, histology and culture (91% vs 66%,43% and 37%, respectively; P = 0.01, <0.001, <0.001,respectively), but similar to that of serology (94%) and UBT (94%). Additionally, PCR exhibited a greater specificity than serology (100% vs 65%, P<0.01). However, the specificity of PCR did not differ from that of other tests.Further analysis revealed significant differences in the sensitivities of RUT, culture, histology and PCR between the patients with and those without blood in the stomach (P<0.01, P = 0.09,P<0.05, and P<0.05, respectively).CONCLUSION: PCR is the most accurate method among the biopsy-based tests to detect H pylori infection in patients with bleeding peptic ulcers. Blood may reduce the sensitivities of all biopsy-based tests. | Ching-ChuLo Kwok-HungLai Nan-JingPeng Gin-HoLo Hui-HwaTseng Chiun-KuLin Chang-BihShie Chao-MingWu Yu-ShanChen Wen-KeuiHuang AngelaChen Ping-IHsu | 2005 | World Journal of Gastroenterology2005，11，25： | 4 |
| 2 | Association of the myeloperoxidase ^(468)G→K polymorphism with gastric inflammation and duodenal ulcer risk显示文摘AIM: To eluddate the relations between the myeloperoxidase -468G→A polymorphism and the development of duodenal ulcer (DU), and to investigate the impacts of this host genetic polymorphism on the histopathological featuresof Helicobacter pylori ( H pylori)-related gastritis. METHODS: In a case-control study of 115 consecutive DU patients and 182 controls, the myeloperoxidase-468G→A polymorphism was genotyped. Additionally, gastric mucosal changes were examined according to the updated Sydney System.RESULTS: The two study groups differed in the distributionsof myelperoxidase genotypes (P= 0.008). All six individuals carrying myeloperoxidase A/A genotypes were in the DU group. The carriage of myeloperoxidase allele A and H pylori infection were associated with an increased risk of DU with odds ratios (OR) of 2.3 and 5.8, respectively. Thecombined risk of the carriage of myeloperoxidase allele A and H pylori infection for DU was 8.7 (95% CI, 3.5-21.8). In the H pylori-infected individuals, allele A carriers displayed higher bacterial density scores (P = 0.04) inthe antrum than did non-carriers.CONCLUSION: This work verifies for the first time the association of myeloperoxidase-468G→A polymorphism with antral H pyloridensity and DU disease. The mechanisms underlying this genetic polymorphism in developing DU disease merit further investigations. | Ping-IHsu Jyh-JenJwo Hui-HwaTseng Kwok-HungLai Gin-HoLo Ching-ChuLo Chung-JenWu Seng-KeeChuah II-RanHwang Jin-LiangChen Yu-ShanChen AngelaChen | 2005 | World Journal of Gastroenterology2005，11，18： | 2 |
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