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3篇 您的检索式:作者名="Andrea Weeks"
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1Microfluidic PCR-based target enrichment: A case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar显示文摘开发有效、成本效率的 multilocus 为被子植物种类的原子数据集是对 systematics 社区的持续挑战。这里,我们为基于 PCR 的目标丰富描述 91 个原子标记的一个新奇集合的发展和确认。用 microfluidic PCR 和 Illumina MiSeq,我们产生了原子,为 96 种类的 subgenomic 图书馆同时并且为 ca 的全部的费用定序他们。$6000 美金。近似,这些的一半花费包括可重用的试剂(定序教材的教材, barcodes,和习俗) 并且 taxon 采样能被一个数量级增加最大化定序深度效率。在其他的目标丰富策略上的 microfluidic PCR 的原则利益是它绕过昂贵的图书馆准备。在定序以后,我们评估了 loci 的能力决定在二以内的种类水平关系最近散发了地方性的 Madagascan Commiphora Jacq 的系。(Burseraceae ) 种类。我们的结果表明那(i) 有效原子标记能从这些公开可得到的数据集为非模型被子植物 taxa 被设计;(ii ) 定序的高产量跟随的那 microfluidic PCR 扩大能与最小的资源投资由地点顺序数据矩阵生产高度完全的 taxon;并且(iii ) 这些众多的原子 phylogenomic 标记能在 Commiphora 以内改进我们种系发生的关系的理解。我们提供进行中的活动的一个提要提高这 microfluidic 通过定序深度的更宽广的教材试金, multiplexing,和增加的效率的基于 PCR 的目标丰富策略。Morgan R. Gostel Kiera A. Coy Andrea Weeks 2015Journal of Systematics and Evolution2015,53,5:3
2Molecular phylogenetic analysis of Leibnitzia Cass.(Asteraceae:Mutisieae:Gerbera-complex),an Asian-North American disjunct genus显示文摘Leibnitzia comprises six species of perennial herbs that are adapted to high elevation conditions and is one of only two Asteraceae genera known to have an exclusively disjunct distribution spanning central to eastern Asia and North America.Molecular phylogenetic analysis of Leibnitzia and other Gerbera-complex members indicates that Leibnitzia is monophyletic,which is in contrast with our expectation that the American Leibnitzia species L. lyrata and L.occimadrensis would be more closely related to another American member of the Gerbera-complex,namely Chaptalia.Ancestral area reconstructions show that the historical biogeography of the Gerbera-complex mirrors that of the entire Asteraceae,with early diverging lineages located in South America that were followed by transfers to Africa and Eurasia and,most recently,to North America.Intercontinental transfer of Leibnitzia appears to have been directed from Asia to North America.Independent calibrations of nuclear(ribosomal DNA internal transcribed spacer region)and chloroplast(trnL-rpl32 intron)DNA sequence data using relaxed clock methods and either mean rate or fossil-based priors unanimously support Miocene and younger divergence times for Gerbera-complex taxa.The ages are not consistent with most Gondwanan vicariance episodes and,thus,the global distribution of Gerbera-complex members must be explained in large part by long-distance dispersal.American species of Leibnitzia are estimated to have diverged from their Asian ancestor during the Quaternary(ca.2 mya)and either migrated overland to North America via Beringia and retreated southwards along high elevation corridors to their present location in southwestern North America or were dispersed long distance.Kristen E.BAIRD Vicki A.FUNK Jun WEN Andrea WEEKS 2010Journal of Systematics and Evolution2010,48,3:2
3The phylogenetic history and biogeography of the frankincense and myrrh family (Burseraceae) based on nuclear and chloroplast sequence data显示文摘Andrea Weeks Douglas C. Daly Beryl B. Simpson 2004Molecular Phylogenetics and Evolution2004,,1:1
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