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| 1 | Dense coding scheme using superpositions of Bell-states and its NMR implementation显示文摘Dense coding using superpositions of Bell-states is proposed. The generalized Grover's algorithm is used to prepare the initial entangled states, and the reverse process of the quantum algorithm is used to determine the entangled state in the decoding measurement. Compared with the previous schemes, the superpositions of two Bell-states are exploited. Our scheme is demonstrated using a nuclear magnetic resonance (NMR)quantum computer. The corresponding manipulations are obtained. Experimental results show a good agreement between theory and experiment. We also generalize the scheme to transmit eight messages by introducing an additional two-state system. | ZHANG Jingfu, XIE Jingyi, DENG Zhiwei & LU Zhiheng Key Laboratory for Quantum Information and Measurements, Department of Physics, Tsinghua University, Beijing 100084, China Center for Quantum Information, Tsinghua University, Beijing 100084, China Department of Materials Science and Engineering, Beijing Normal University, Beijing 100875, China Testing and Analytical Center, Beijing Normal University, Beijing 100875, China Department of Physics, Beijing Normal University, Beijing 100875, China | 2005 | Science China(Physics,Mechanics & Astronomy)2005，48，1： | 18 |
| 2 | Primary structure and configuration of tea polysaccharide显示文摘The monosaccharide composition of a tea polysaccharide (TGC) was determined by GC-MS method. Furthermore, the primary structure of tea polysaccharide and its configura-tion in the aqueous solution were investigated utilizing a combination of classical chemical methods and modern instrumental techniques including GC-MS, Proton NMR, UV and CD. The results indicate that TGC consists of 6 monosaccharides: Rha, Ara, Xyl, Glu, Man and Gal. The configuration of TGC in water solution is proposed to be an ordered helix. The possible primary structure of TGC was outlined as below: the basic structure of the main chain consists of Rha, Glu and Gal units. All three monosaccharides can potentially be connected to branch chains consisting of mainly Ara, and the linkages could be in b1→2, b 1→3, b 2→3 forms. When branch chain is absent in the basic structure of the main chain the linkage consists of only b 1→3; Xyl exists at the terminal end of either the main chain or the branch chain with b 1→ linkage. | ZHOU Peng1, XIE Mingyong1, NIE Shaoping1 & WANG Xiaoru2 1. Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China 2. Key Laboratory of Analytical Science of Ministry of Education, Xiamen University, Xiamen 361005, China | 2004 | Science China(Life Sciences)2004，47，5： | 12 |
| 3 | Excretion of Four Catechins in Tea Polyphenols in Rats显示文摘Objective To investigate excretion profiles of the four major anti-oxidant active catechins, (-) epigallo-catechin-3-gallate (EGCG), (-) epicatechin-3-gallate (ECG), (-) epigallocatechin (EGC), and epicatechin (EC) in tea polyphenols (TP) in rats in order to provide experimental data for clinical uses and development of TP as a novel drug. Methods The above four catechins in urine, bile, and feces were simultaneously determined by high performance liquid chromatography coupled with ultraviolet absorption detector (HPLC-UV) assay with a binary gradient elution. The samples were extracted by ethyl acetate prior to HPLC. The quantification was carried out by peak area internal standard method. Following iv dosing TP 100 mg/kg to rats, the samples were collected at different time intervals up to 8 h (urine and bile) and 24 h (feces). Results The urinary Ae, 0-8 h (cumulative excretion amount over 8 h) of EGCG, ECG, EGC, and EC were, on the average, 150.83, 30.75, 116.69, and 254.56 μg, corresponding to fe, 0-8 h (cumulative excretion fraction of dose over 8 h) of 1.45%, 0.84%, 7.88%, and 10.73%, respectively; the biliary Ae, 0-8 h were 12.61, 42.64, 6.61, and 1.24 μg, corresponding to the fe, 0-8 h of 0.12%, 1.16%, 0.45%, and 0.053%,respectively. For fecal excretion, only EGCG and EGC were detected with Ae, 0-24 h of 7.38 μg (fe, 0-24 h of 0.07%) and 157 μg (fe, 0-24 h of 9.99 %), respectively. The fe, total (the total fe of 3 excretory routes) were 18.32%, 10.78%, 2.00%, and 1.64% for EGC, EC, ECG, and EGCG, respectively. Conclusion EGCG and EC are mainly excreted in urine, ECG in bile, and EGC in feces by reference to their Ae and fe. The excretion of the four catechins based on fe, total is ranked in order of EGC > EC > ECG > EGCG. Only small amount of four catechins are recovered in urine, bile, and feces, indicating an extensive metabolic conversion of catechins in the rat body. | LI Qiu-sha1, ZOU Ling-li1, FU Ting1, HAN Guo-zhu1, L Li1, ZHOU Qin1, LIN Ya-fang1, LI Nan2 1. Department of Clinical Pharmacology, Dalian Medical University, Dalian 116044, China 2. Department of Analytical Chemistry, Dalian University of Science & Technology, Dalian 116023, China | 2009 | Chinese Herbal Medicines2009，1，1： | 10 |
| 4 | LC-MS/MS for Simultaneous Determination of Four Major Active Catechins of Tea Polyphenols in Rat Plasma and Its Application to Pharmacokinetics显示文摘Objective To develop a liquid chromatography technique coupled with tandem mass spectrometry (LC-MS/MS) for simultaneous determination of four active catechins EGCG, ECG, EGC, and EC of tea polyphenols (TP) in rat plasma in order to further study its multi-component pharmacokinetics. Methods Following a single step liquid-liquid extraction of plasma samples with ethyl acetate, the four catechins were separated on a Hypersil ODS C18 column using an isocratic mobile phase composed of methanol-water (30︰70). The detection using a mass spectrometer was performed under negative ESI in the MRM mode. The analytes were identified by reference to both MRM and tR values and quantified using peak area internal standard method. Results The method was shown to be specific without interference from matrix, metabolites, and impurities present in TP raw material and to be sensitive with LOD and LOQ of 1.5 and 10 ng/mL (EGCG) as well as 0.75 and 5 ng/mL (ECG, EGC, and EC). A good linearity was obtained over a wide range of 10-10 000 ng/mL for EGCG and 5-5000 ng/mL for other three catechins (r > 0.996). The method was validated to be reproducible and reliable, as evidenced by intra-batch and inter-batch precision of less than 10% and 11%, accuracy of 97.13%-106.05% and 99.22%-103.14%, respectively. The recovery of extraction ranged from 72.74% to 89.13%, matrix effect from 88.76% to 105.97% for four catechins. The method was successfully used to study the pharmacokinetics of TP iv administered to rats at a dose of 100 mg/kg. Conclusion This method is shown to completely meet requirements for the multi-component pharmacokinetic study of TP in rats. | WANG Chang-yuan1, LI Qiu-sha1, HAN Guo-zhu1, ZOU Ling-li1, LV Li1, ZHOU Qin1, LI Nan2 1. College of Pharmacy, Dalian Medical University, Dalian 116044, China 2. Department of Analytical Chemistry, Dalian University of Sciences and Technology, Dalian 116023, China | 2010 | Chinese Herbal Medicines2010，2，4： | 9 |
| 5 | Method Development of Enantiomer Separations by Affinity Capillary Electrophoresis,Cyclodextrin Electrokinetic Chromatography and Capillary Electrophoresis-Mass Spectrometry显示文摘Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affinity CE) with some proteins and by cyclodextrin electrokinetic chromatography (CDEKC) with some charged cyclodextrins (CDs). Many successful enantiomer separations are demonstrated from our study in this review article. In the enantiomer separations by affinity CE, the deterioration of detection sensitivity was observed under high concentration of the protein in running solutions. The partial filling technique was practically useful to solve the serious problem. It allowed operation at high protein concentrations, such as 500 μmol/L, without the detection problem. Charged CDs had several advantages for the enantiomer separations over neutral ones. Strong electrostatic interactions between a charged CD and oppositely charged analytes should be effective for the formation of the complex. A large difference in electrophoretic mobility between the free analyte and the inclusion complex should also enhance the enantiomeric resolution. In CE mass spectrometry (CE MS), the partial filling technique was applied to avoid the introduction of nonvolatile chiral selectors into the CE MS interface. By replacing the nonvolatile electrolytes in the running buffer by volatile ones, the separation conditions employed in CE with the UV detection method could be transferred to CE MS. | TANAKA Yoshihide (Department of Analytical Chemistry, Nippon Boehringer Ingelheim Co. Ltd., 3 10 1, Yato, Kawanishi, Hyogo 666 0193, Japan) | 2002 | 色谱2002，20，4： | 8 |
| 6 | Genomic research for important pathogenic bacteria in China显示文摘Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point.We can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug targets.The newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease. | YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 Laboratory of Analytical Microbiology,State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China 2 Department of Medical Microbiology and Parasitology,Institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China 3 State Key Laboratory for Molecular Virology and Genetic engineering,Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing 100176,China 4 State Key Laboratory for Infectious Disease Prevention and Control,Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China 5 Beijing Genomics Institute at Shenzhen,Shenzhen 518083,China | 2009 | Science China(Life Sciences)2009，52，1： | 8 |
| 7 | Implementation of a multiple round quantum dense coding using nuclear magnetic resonance显示文摘A multiple round quantum dense coding scheme based on the quantum phase estimation algorithm is proposed and implemented in a three qubit nuclear magnetic resonance (NMR) quantum computer. Using an m + 1 qubit system, Bob can transmit one of 2m+1 messages to Alice, through manipulating only one qubit and exchanging it between Alice and Bob for m rounds. The information capacity is enhanced to m + 1 bits as compared to m bits in a classical scheme. The scheme has been demonstrated in NMR system, and the experimental results show a good agreement between theory and experiment. | ZHANG Jingfu1, XIE Jingyi2, WANG Chuan1, DENG Zhiwei3, LU Zhiheng4, & LONG Guilu1,5 1. Key Laboratory For Quantum Information and Measurements of MOE, Department of Physics, Tsinghua University, Beijing 100084,China 2. Department of Materials Science and Engineering, Beijing Normal University, Beijing 100875, China 3. Testing and Analytical Center, Beijing Normal University, Beijing 100875,China 4. Department of Physics, Beijing Normal University, Beijing 100875, China 5. Key Laboratory For atomic and Molecular Nanosciences, Tsinghua University, Beijing 100084,China | 2005 | Science China(Physics,Mechanics & Astronomy)2005，48，6： | 8 |
| 8 | “92·8”高含沙洪水在黄河下游演进特性分析显示文摘“92.8”洪水位异常高的主要原因:一是前期连续几年枯水,造成河床严重淤积;二是本场洪水边滩淤积,河道过水面积减小。花园口站洪峰流量大于上站的主要原因是:主槽强烈冲刷,水位大幅度下降,漫滩水迅速回归主槽。 | Analytical Group of'August 92'Flood of YRCC | 1993 | 人民黄河1993，15，8： | 6 |
| 9 | Sensitive biosensing strategy based on functional nanomaterials显示文摘The first decade of the 21st century has been labeled as 'the sensing decade'. The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and electronic biosensors. A lot of works have fo- cused on the biofunctionalization of different nanomaterials, such as metal nanoparticles, semiconductor nanoparticles and carbon nanostructures, by physical adsorption, electrostatic binding, specific recognition or covalent coupling. These biofunc- tionalized nanomaterials can be used as catalysts, electronic conductors, optical emitters, carriers or tracers to obtain the ampli- fied detection signal and the stabilized recognition probes or biosensing interface. The designed signal amplification strategies have greatly promoted the development of stable, specific, selective and sensitive biosensors in different fields. This review in- troduces some novel principles and detection strategies in the area of biosensing, based on functional nanomaterials. The gen- eral methods for biofunctionalization of nanomaterials with biomolecules and their biosensing application in immunoassay of protein, DNA detection, carbohydrate analysis and cytosensing are also described. | JU HuangXian State Key Laboratory of Analytical Chemistry for Life Science Department of Chemistry, Nanjing University, Nanjing 210093, China | 2011 | Science China Chemistry2011，54，8： | 6 |
| 10 | Progress in the development of techniques based on light scattering for single nanoparticle detection显示文摘Nanoparticles have recently attracted extensive attention in view of their great potential in biomedicine and bioanalytical ap- plications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparticles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles. | ZHU ShaoBin, WANG Shuo, YANG LingLing, HUANG TianXun & YAN XiaoMei Department of Chemical Biology, College of Chemistry and Chemical Engineering Key Laboratory for Chemical Biology of Fujian Province Key Laboratory of Analytical Science, Xiamen University, Xiamen 361005, China | 2011 | Science China Chemistry2011，54，8： | 5 |
| 11 | Magnetic particles and cadmium sulfide nanoparticles tagging for signal-amplifying detection of nucleic acids显示文摘A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays. | DONG XiaoYa, ZHAO WeiWei, XU JingJuan & CHEN HongYuan State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China | 2011 | Science China Chemistry2011，54，8： | 4 |
| 12 | Determination of abscisic acid based on the fluorescent quenching of quantum dots显示文摘The quenching mechanism of the fluorescence of quantum dots by abscisic acid has been systematically investigated.The quenching constant KSV = 5.1 × 1011 / M was obtained under the optimized condition.On the basis of that,a very sensitive method for the determination of abscisic acid has been developed.The linear equation was F0/F = 0.9309 + 0.5072 C(pmol/L) and its linear range was 0.2-3.0 pmol/L with a correlation coefficient of 0.9939.The limit of detection was 0.09 pmol/L. | ZHANG Lei,GUAN HongLiang & HE ZhiKe Key Laboratory of Analytical Chemistry for Biology and Medicine,Ministry of Education College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China | 2010 | Science China Chemistry2010，53，1： | 4 |
| 13 | Rhodamine B doped silica nanoparticle labels for protein microarray detection显示文摘A core-shell Rhodamine B-doped SiO2 nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule. The doped nanoparticles were further conjugated with streptavidin and the resulting nanoparticles were used in the detection of reverse-phase protein microarrays, in which human IgG of various concentrations was first immobilized on aldehyde-modified glass slides and then biotinlyated goat anti human IgG as well as the labeled nanoparticles were sequentially conjugated. The calibration curve is linear over the range from 800 fg to 500 pg and the limit of detection is 100 fg, which is 8 times lower than that of streptavidin-labeled Cy3 fluorescent dyes. The dyedoped SiO2 nanoparticles show potentials for the protein array detection. | WANG Ying1, LI ZhongHui1, ZHONG WenYing2, LI Hui2, XU DanKe1 & CHEN HongYuan1 1Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China 2Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China | 2010 | Science China Chemistry2010，53，4： | 3 |
| 14 | A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum显示文摘Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained. | ZHU Jun, WANG FangJun, DONG XiaoLi, YE MingLiang & ZOU HanFa Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | 2010 | Science China Chemistry2010，53，4： | 3 |
| 15 | Immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence for the detection of abscisic acid显示文摘Abscisic acid (ABA) is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical method for the ABA detection is urgently required for further investigation of ABA signaling. In this work, an immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence has been developed for the detection of ABA. The result could be read out in 30 min at least, with the simplified procedure of immunomagnetic assay. Under the optimized condition, a linear range from 1 pM to 10 nM was obtained. An unexpected result induced by the dose hook effect was discussed. This method provided the high selectivity for ABA over other components that might be contained in real samples. | ZHOU GuoHua, WANG Ping, YUAN Ju, QIU Ting & HE ZhiKe Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China | 2011 | Science China Chemistry2011，54，8： | 3 |
| 16 | Expression and function analysis of the metallo-thionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast显示文摘The cDNA of the metallothionein-like (MT-like) gene from Festuca rubra cv. Merlin was optimized with bias codon of Chlamydomonous reinhardtii chloroplast genome. The optimized MT-like gene was de-livered into C. reinhardtii chloroplast and the transgenic strains expressing MT-like gene was obtained. PCR-Southern blot and RT-PCR-Southern blot analysis demonstrated that the MT-like gene was inte-grated into chloroplast genome of C. reinhardtii and expressed at the transcriptional level. The cad-mium binding capacity of the transgenic C. reinhardtii was determined by hydride generation-atomic fluorescence spectrometry (HG-AFS) and the binding properties were analyzed. Results showed that the transgenic C. reinhardtii expressing the MT-like gene exhibited remarkably higher Cd2+ binding capacity and grew to higher densities at toxic Cd2+ concentrations (40-100 μmol/L) than the wild type strain, and that the IC50 of Cd2+ (3-d treating ) to algal cell growth of transgenic strain was 55.43% higher than that of the wild type strain, indicating that the Cd2+ binding capacity and Cd2+ tolerance of C. reinhardtii was enhanced through the expression of the foreign MT-like gene in chloroplast. | HAN SiHai1,2, HU ZhangLi1 & LEI AnPing1 1 Institute of Eco-Environment, College of Life Science, Shenzhen University, Shenzhen 518060, China 2 Research Center for Analytical Instrumentation, State Key Lab of Industrial Control Technology, Institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, China | 2008 | Science China(Life Sciences)2008，51，12： | 3 |
| 17 | Study on New Spectrophotometric Determination of Arsenic (V) with Chlorpromazine显示文摘It was shown that a new sensitive spectrophotometric method for the determination oftrace arsenic with chlorpromazine by the formation of heteropoly arsenomolybdic chlorpromazinecomplex in aqueous phase. | Shao Min LIU Yue Qin XIE Yu Biao WANG(Department of Analytical Chemistry,Hefel Institute of Economics and Technology. Hefel 230052,)(Department of Chemistry, Anhui Instructive College. Hefel 230061) | 1999 | Chinese Chemical Letters1999，10，2： | 3 |
| 18 | Electrochemiluminescence from tris(2,2′-bipyridyl) ruthenium (Ⅱ) in the presence of aminocarboxylic acid co-reactants显示文摘Due to the highly sensitive electrochemiluminescence (ECL), tris(2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+) is often used in the field of bioarrays with the help of co-reactants. However, the generally used co-reactant, tripropylamine (TPA), is toxic, corrosive and volatile. Therefore, the search for safe, sensitive and economical co-reactants is critical. Herein, three aminocarboxylic acids, ethylenediamine-tetraacetic acid (EDTA), nitrilotriacetic acid (NTA), and 2-hydroxyethylethylene diaminetriacetic acid (HEDTA), have been investigated as potential co-reactants for promoting Ru(bpy)32+ ECL behaviour. A possible ECL mechanism is also presented. The experimental results suggested that the co-reactants have a different ECL behaviour compared to TPA, such as different pH- and surfactant-responses. The detection limits of Ru(bpy)32+ using NTA, EDTA and HEDTA as co-reactants are 1, 60 and 680 fmol·L-1, respectively. The results indicate that NTA has a much higher efficiency than TPA to excite Ru(bpy)3 2+ ECL under their own optimal conditions. NTA could be widely used in many fields because it is less toxic, corrosive and volatile than TPA. Moreover, using Ru(bpy)3 2+ ECL, a sensitive method for the detection of aminocarboxylic acids is also developed. An improvement of four orders of magnitude in detection limits is obtained for EDTA compared to the known Ru(bpy) 3 2+ chemiluminescent methods. | YIN XueBo, SHA BeiBei & HE XiWen Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China | 2009 | Science China Chemistry2009，52，9： | 2 |
| 19 | Dimethyl sulfide in the atmospheric surface layer of the Equatorial Pacific Ocean显示文摘This paper reports a case study of atmospheric stability effect on dimethyl sulfide(DMS) concentration in the air. Investigation includes model simulation and field measurements over the Pacific Ocean. DMS concentration in surface sea water and in the air were measured during a research cruise from Hawaii to Tahiti. The diurnal variation of air temperature over the sea surface differed from the diurnal cycle of sea surface temperature because of the high heat capacity of sea water. The diurnal cycle of average DMS concentration in the air was studied in relation to the atmospheric stability parameter and surface heat flux. All these parameters had minima at noon and maxima in the early morning. The correlation coefficient of the air DMS concentration with wind speed (at 15 m high) was 0. 64. The observed concentrations of DMS in the equatorial marine surface layer and their diurnal variability agree well with model simulations. The simulated results indicate that the amplitude of the cycle and the | Li Xingsheng F. Parungo, C. Nagamoto and S. Hoyt Chinese Academy of Meteorological Sciences, State Meteorological Administration, Beijing 100081 , China Air Resources Laboratory, NOAA/ERL, Boulder, Colorado, U. S. A. Environmental Analytical Service, San Luis Obispo, California, U. S. A. | 1993 | Acta Oceanologica Sinica1993，12，1： | 2 |
| 20 | Fabrication of the DNA/poly(3-methylthiophene) composite film modified electrode and its application for the study on the voltammetric behavior and determination of 8-hydroxy-2'-deoxyguanosine显示文摘A composite film of DNA/poly(3-methylthiophene)(P3MT) modified glassy carbon electrode(GCE) has been fabricated by electro-deposition method.P3MT film was first electropolymerized at the GCE and the DNA layer was then immobilized on the P3MT layer by electrochemical method.The voltammetric behavior of 8-hydroxy-2'-deoxyguanosine(8-OH-dG) at the composite film modified electrode was studied.The effects of scan rates,pH and the interference of uric acid(UA) on the voltammetric behavior and detection of 8-OH-dG were also discussed.The experimental results suggest that the electrochemical behavior of 8-OH-dG at the composite film modified electrode was greatly improved due to the combination of the advantages of P3MT and DNA.In 0.1 M pH 7.0 phosphate buffer solution(PBS),the anodic peak currents of 8-OH-dG were linear with the 8-OH-dG concentration in two intervals,viz.0.28―4.2 μM and 4.2―19.6 μM.The detection limit of 56 nM 8-OH-dG could be estimated(S/N=3).This proposed composite film modified electrode shows excellent reproducibility and stability.It may have the potential application for the detection of 8-OH-dG in human urine. | WANG YanHuai,LI Jing,LIU Yan,MA RongNa,JIA WenLi,CUI Hui & WANG HuaiSheng College of Chemistry and Chemical Engineering,Institute of Analytical Chemistry,Liaocheng University,Liaocheng 252059,China | 2009 | Science China Chemistry2009，52，11： | 2 |
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