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| 1 | Changes in growth factor and cytokine expression in biliary obstructed rat liver and their relationship with delayed liver regeneration after partial hepatectomy显示文摘瞄准:在部分肝切除术以后在肝新生上学习妨碍的黄疸的效果。方法: Hepatocyte 生长因素( HGF ),它的受体, 遇见c ,脉管的内皮生长因素( VEGF )和转变生长在肝织物和孤立的肝细胞的 factor-beta1 ( TGF-beta1 ) mRNA 表示被量的反向抄写的聚合酶链反应( RT-PCR )用 LightCycler 在胆汁的阻塞( BO )以后调查。为 desmin 和 alpha 光滑的肌肉肌动朊(alpha-SMA ) 染色的 Immunohistochemical 也被学习。标记的原子抗原(PCNA ) 索引的再生肝重量和增殖的房间,和生长因素表示然后与伴随物在 70% 肝切除术以后被评估在 BO 老鼠或假冒操作老鼠的内部胆汁的排水。结果:肝的 TGF-beta1 mRNA 层次显著地在 BO 以后增加了 14 天,并且进一步与胆汁郁积的持续时间增加了。同时, HGF 和 VEGF 趋于增加,但是不是重要的。在房间孤立, TGF-beta1 mRNA 主要在肝的星形的房间(HSC ) 被发现部分。Immunohistochemical 研究揭示了 HSC (desmin 积极的房间) 的一个增加的数字并且在 BO 以后在门区域激活 HSC (alpha-SMA-positive 房间) 。在一个肝切除术模型,肝新生在 BO 老鼠被推迟,作为与假冒操作老鼠相比。在肝切除术,和更早的 HGF mRNA 山峰沉醉于 BO 老鼠以后, TGF-beta1 mRNA 是显著地起来调整的直到 48 h。结论:BO 导致 HSC 增长和激活,导致 TGF-beta1 mRNA 的起来规定和在肝的 HGF mRNA 的抑制。这些改变的表示模式可以强烈与妨碍的黄疸在肝切除术以后涉及推迟的肝新生。 | Hironobu Makino Hiroaki Shimizu Hiroshi Ito Fumio Kimura Satoshi Ambiru Akira Togawa Masayuki Ohtsuka Hiroyuki Yoshidome Atsushi Kato Hideyuki Yoshitomi Shigeaki Sawada Masaru Miyazaki | 2006 | World Journal of Gastroenterology2006，12，13： | 32 |
| 2 | Vascular endothelial growth factor and angiopoietins regulate sinusoidal regeneration and remodeling after partial hepatectomy in rats显示文摘AIM: To study the regulatory mechanisms of sinusoidal regeneration after partial hepatectomy.METHODS: We investigated the expression of angiopoietin (Ang)-1, Ang-2, Tie-2, and vascular endothelial growth factor (VEGF) in regenerating liver tissue by quantitative reverse-transcription polymerase chain reaction (RT-PCR) using a LightCycler (Roche Diagnostics) and also immunohistochemical staining after 70% hepatectomy in rats. In the next step, we isolated liver cells (hepatocytes,sinusoidal endothelial cell (SEC), Kupffer cell, and hepatic stellate cells (HSC)) from regenerating liver tissue by in situ collagenase perfusion and counterflow elutriation,to determine potential cellular sources of these angiogenic factors after hepatectomy. Proliferation and apoptosis of SECs were also evaluated by proliferating cell nuclear antigen (PCNA) staining and the terminal deoxynucleotidyl transferase d-uridine triphosphate nick end labeling (TUNEL) assay, respectively.RESULTS: VEGF mRNA expression increased with a peak at 72 h after hepatectomy, decreasing thereafter.The expression of Ang-1 mRNA was present at detectable levels before hepatectomy and increased slowly with a peak at 96 h. Meanwhile, Ang-2 mRNA was hardly detected before hepatectomy, but was remarkably induced at 120 and 144 h. In isolated cells, VEGF mRNA expression was found mainly in the hepatocyte fraction.Meanwhile, mRNA for Ang-1 and Ang-2 was foundin the SEC and HSC fractions, but was more prominent in the latter. The PCNA labeling index of SECs increased slowly,reaching a peak at 72 h, whereas apoptotic SECs were detected between 120 h and 144 h.CONCLUSION: Ang-Tie system, together with VEGF,plays a critical role in regulating balance between SEC proliferation and apoptosis during sinusoidal regeneration after hepatectomy. However, the VEGF system plays a more important role in the early phase of sinusoidal regeneration than angiopoietin/Tie system. | Hiroaki Shimizu Noboru Mitsuhashi Masayuki Ohtsuka Hiroshi Ito Fumio Kimura Satoshi Ambiru Akira Togawa Hiroyuki Yoshidome Atsushi Kato Masaru Miyazaki | 2005 | World Journal of Gastroenterology2005，11，46： | 17 |
| 3 | Effects of perioperative protease inhibitor on inflammatory cytokines and acutephase proteins in patienswith hepatic resection显示文摘 | Ambiru S MiyazakiM SesadaK etal | 2000 | Dig Surg2000，17，： | 1 |
| 4 | Effect of perioperative protease inhibitor on inflammatory cytokines and phase proteins in patients with hepatic resection显示文摘 | AMBIRU S MIYAZAKI M SASADA K | 2000 | Dig Surg2000，17，4： | 1 |
| 5 | Muhidrug resistance gene(MDR-1)expression as a useful prognostic factor in patients with human hepatocellular carcinoma after surgical resection显示文摘 | Kato A Miyazaki M Ambiru S | 2001 | J Surg Oncol2001，78，2： | 1 |
| 6 | Effects of perioperative protease inhibitor on inflammatory cytokines and acute-phase proteins in patients with hepatic resection显示文摘 | AMBIRU S MIYAZAKI M SASADA K | 2000 | DigSurg2000，17，4： | 1 |
| 7 | Effects of perioperarive protease inhibitor on inflammatory cytokines and acutephase proteins in patients with hepatic resection 显示文摘 | Ambiru S Miyazaki M Sasada K | 2000 | Dig Surg2000，17，4： | 1 |
| 8 | Aggressive surgical approaches to hilar cholangiocarcinoma: Hepatic or local resection?显示文摘 | Masaru Miyazaki Hiroshi Ito Koji Nakagawa Satoshi Ambiru Hiroaki Shimizu Yoshiaki Shimizu Atsushi Kato Shunta Nakamura Hideyuki Omoto Nobuyuki Nakajima Fumio Kimura Toshikazu Suwa | 1998 | Surgery1998，，2： | 1 |
| 9 | Adjuvant regional chemotherapy after hepatic resection for colorectal metasases显示文摘 | Miyazaki M Ito H | 1999 | Br J Surg1999，86，8： | 1 |
| 10 | Radical surgery for advanced gallbladder carcinoma 显示文摘 | Mivazaki M Itoh H Ambiru S | 1996 | Br J Surg1996，83，4： | 1 |
| 11 | Effects of perioperative prote-ase inhibitor on inflammatory cytokines and acute-phase proteins inpatients with hepatic resection显示文摘 | Ambiru S Miyazaki M Sasada K | 2000 | Dig Surg2000，17，4： | 1 |
| 12 | Benefits and limits of hepatic resection for gastric metastases显示文摘 | Satoshi Ambiru Masaru Miyazaki Hiroshi Ito Koji Nakagawa Hiroaki Shimizu Hiroyuki Yoshidome Yoshiaki Shimizu Nobuyuki Nakajima | 2001 | The American Journal of Surgery2001，，3： | 1 |
| 13 | Radical surgery for advanced gall-bladder carcinoma显示文摘 | Miyazaki M Itoh H Ambiru S | 1996 | Br J Surg1996，83，4： | 1 |
| 14 | Effects of perioperative protease inhibitor on inflammatory cytokines and acute phase proteins in patients with hepatic resection显示文摘 | Ambiru S Miyazaki M Sasada K | 2000 | Dig Surg2000，17，4： | 1 |
| 15 | Analysis of risk factors associated with complications of hyperbaric oxygen therapy显示文摘 | Ambiru S Furuyama N Aono M | 2008 | J Crit Care2008，23，3： | 1 |
| 16 | Radical surgery for advanced gallbladder carcinoma显示文摘 | Itoh H Ambiru S | 1996 | Br J Surg1996，83，： | 1 |
| 17 | Benefits and limits of hepatic resection for gastric metastases 显示文摘 | Ambiru S Miyazaki M lto H | 2001 | Am J Surg2001，181，27： | 1 |
| 18 | A prospective study of prognost ic value of type IV collagenase activity in colorectal cancer tissue显示文摘 | Ambiru S Migazaki M Ito H | | Dig Dis Sci0，42，8： | 1 |
| 19 | Effects of perioperative protease inhibitor on inflammatory cytokines and acute-phase protients with hepatic resection显示文摘 | Ambiru S Miyazaki M Sasada K | 2000 | Dig Surg2000，17，4： | 1 |
| 20 | Benefits and limits of hepatic resection for gastric metastases 显示文摘 | Ambiru S Miyazaki M Ito H | 2001 | Am J Surg2001，181，： | 1 |
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