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| 1 | High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay显示文摘Viruses are extremely heterogeneous entities; the size and the nature of their genetic information, as well as the strategies employed to amplify and propagate their genomes, are highly variable. However, as obligatory intracellular parasites, replication of all viruses relies on the host cell. Having co-evolved with their host for several million years, viruses have developed very sophisticated strategies to hijack cellular factors that promote virus uptake, replication, and spread. Identification of host cell factors(HCFs) required for these processes is a major challenge for researchers, but it enables the identification of new, highly selective targets for anti viral therapeutics. To this end, the establishment of platforms enabling genome-wide high-throughput RNA interference(HT-RNAi) screens has led to the identification of several key factors involved in the viral lifecycle. A number of genome-wide HT-RNAi screens have been performed for major human pathogens. These studies enable first inter-viral comparisons related to HCF requirements. Although several cellular functions appear to be uniformly required for the life cycle of most viruses tested(such as the proteasome and the Golgi-mediated secretory pathways), some factors, like the lipid kinase Phosphatidylinositol 4-kinase Ⅲα in the case of hepatitis C virus, are selectively required for individual viruses. However, despite the amount of data available, we are still far away from a comprehensive understanding of the interplay between viruses and host factors. Major limitations towards this goal are the low sensitivity and specificity of such screens, resulting in limited overlap between different screens performed with the same virus. This review focuses on how statistical and bioinformatic analysis methods applied to HTRNAi screens can help overcoming these issues thus increasing the reliability and impact of such studies. | Sandeep Amberkar Narsis A Kiani Ralf Bartenschlager Gualtiero Alvisi Lars Kaderali | 2013 | World Journal of Virology2013，2，2： | 9 |
| 2 | How virus persistence can initiate the tumorigenesis process显示文摘Human oncogenic viruses are defined as necessary but not sufficient to initiate cancer. Experimental evidence suggests that the oncogenic potential of a virus is effective in cells that have already accumulated a number of genetic mutations leading to cell cycle deregulation. Current models for viral driven oncogenesis cannot explain why tumor development in carriers of tumorigenic viruses is a very rare event, occurring decades after virus infection. Considering that viruses are mutagenic agents per se and human oncogenic viruses additionally establish latent and persistent infections, we attempt here to provide a general mechanism of tumor initiation both for RNA and DNA viruses, suggesting viruses could be both necessary and sufficient in triggering human tumorigenesis initiation. Upon reviewing emerging evidence on the ability of viruses to induce DNA damage while subverting the DNA damage response and inducing epigenetic disturbance in the infected cell, we hypothesize a general, albeit inefficient hit and rest mechanism by which viruses may produce a limited reservoir of cells harboring permanent damage that would be initiated when the vi-rus first hits the cell, before latency is established. Cells surviving virus generated damage would consequently become more sensitive to further damage mediated by the otherwise insufficient transforming activity of virus products expressed in latency, or upon episodic reactivations(viral persistence). Cells with a combination of genetic and epigenetic damage leading to a cancerous phenotype would emerge very rarely, as the probability of such an occurrence would be dependent on severity and frequency of consecutive hit and rest cycles due to viral reinfections and reactivations. | Simone Avanzi Gualtiero Alvisi Alessandro Ripalti | 2013 | World Journal of Virology2013，2，2： | 3 |
| 3 | Respiratory mechanics at different PEEP level during general anaesthesia in the elderly: a pilot study 显示文摘 | Marangoni E Alvisi V Ragazzi R | 2012 | Minerva Anesthesiologica2012，78，11： | 1 |
| 4 | An importin alpha/betarecognized bipartite nuclear localization signal mediates targeting of the human herpes simplex virus type 1 DNA polymerase catalytic subunit pUL30 to the nucleus 显示文摘 | Alvisi G Musiani D Jans DA | 2007 | Biochemistry2007，，32： | 1 |
| 5 | A survey of rollback recovery protocols in message passing systems显示文摘 | Elnozahy E N Alvisi L Wang Y M | 2002 | ACM Computing Surveys2002，34，3： | 1 |
| 6 | Tumor-specific nuclear targeting:promises for anti-cancer therapy?显示文摘 | Alvisi G Poon I K Jans D A | 2006 | Drug Resist Updat2006，9，12： | 1 |
| 7 | Structural and optical modification in hafnia oxide thin films related to the momentum parameter transferred by ion beam assistance 显示文摘 | Alvisi M Scagfione S Martelli S | 1999 | Thin Solid Films1999，354，1： | 1 |
| 8 | Structural andoptical properties of silver films deposited by RF magnetron sputtering显示文摘 | Rizzo A Tagliente M A Alvisi M | 2001 | Thin Solid Films2001，396，： | 1 |
| 9 | Metalloporphyrins-modified carbon nanotubes networked films-based chemical sensors for enhanced gas sensitivity 显示文摘 | Penza M Rossi R Alvisi M | 2010 | Sens Actuators B2010，144，2： | 1 |
| 10 | Influence of the assisting-ion-beam parameters on the laser-damage threshold of SiOz films显示文摘 | Alvisi M Nunzio G De Perrone M R | 1999 | Thin Solid Films1999，338，12： | 1 |
| 11 | Ictal characteristics of psychogenic nonepileptic seizures: what we have learned from video/EEG recordings--a literature review 显示文摘 | Mostacci B Bisulli F Alvisi L | 2011 | Epilepsy Behav2011，22，2： | 1 |
| 12 | A survey of rollback recovery protocols in message passing systems显示文摘 | ELNOZAHY E ALVISI L WANG Y | 2002 | ACM Computing Survey2002，33，3： | 1 |
| 13 | Acid salt corrosion in a hydrotreatment plant of a petroleum refinery显示文摘 | ALVISI P P CUNHA LINS V F | 2008 | Engineering Failure Analysis2008，15，8： | 1 |
| 14 | A survey of rollback-recovery protocols in message-passing systems 显示文摘 | Elnozahy E N Alvisi L Wang Y M | 2002 | ACM Computing Surveys2002，34，3： | 1 |
| 15 | Weaning from mechanical ventilation显示文摘 | Volta CA Alvisi V Marangoni E | 2006 | Curr Anaes Crit Care2006，17，6： | 1 |
| 16 | A survey of rollback-recovery protocols in message-passing systems 显示文摘 | Elnozahy E N Alvisi Lorenzo Wang Yi-min | 2002 | ACM Computing Surveys2002，34，3： | 1 |
| 17 | Comparison of serum total sialic acid, C-reactive protein, α 1 -acid glycoprotein and β 2 -microglobulin in patients with non-malignant bowel diseases显示文摘 | G Ricci AD’ Ambrosi D Resca M Masotti V Alvisi | 1995 | Biomedicine & Pharmacotherapy1995，，5： | 1 |
| 18 | Wrapping server-side TCP to mask connection failures显示文摘 | Alvisi L Bressoud T El-Khashab A | 2001 | Infocom2001，1，： | 1 |
| 19 | Tumor-specific nuclear targeting:promises for anti-cancer therapy显示文摘 | ALVISI G POON I K JANS D A | 2006 | Drug Resistance Updates2006，9，1： | 1 |
| 20 | Pt and Pd-nanoclusters funetionalized carbon nanotubes networked films for sub- ppm gas sensors 显示文摘 | I Penza M Rossi R Alvisi M | 2008 | Sensor Actuat B2008，135，： | 1 |
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