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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Popliteal artery entrapment syndrome in an elite rower: sonographie appearances 显示文摘 | Alvarez Rey I Alvarez Rey G Alvero Cruz JR | 2004 | J Ultrasound Med2004，23，12： | 1 |
| 3 | Microtubule binding to Smads may regulate TGF beta activity显示文摘 | Dong C Li Z Alvarez R Jr | 2000 | Mol Cell2000，5，： | 1 |
| 4 | Pickworth kk Safety of antidepressant drugs in the patient with cardiac disease显示文摘 | Alvarez W Jr Pickworth KK | 2003 | Pharmacotherapy2003，23，6： | 1 |
| 5 | SERS detection of environmental pollutants in humic acid- gold nanoparticle composite materials 显示文摘 | ALVAREZ PUEBLA R A DOS SANTOS D S JR AROCA R F | 2007 | Analyst2007，132，12： | 1 |
| 6 | Molecular basis for angiotensin Ⅱ induced increase of chloride/bicarbonate exchange in the myocardium显示文摘 | Alvarez BV Fujinaga J Casey JR | 2001 | Circ Res2001，89，： | 1 |
| 7 | Management of spontaneous congenital chylothorax: oral medium chain triglycerides versus total parenteral nutrition显示文摘 | Fernfindez Alvarez JR Kalache KD Grauel EL | 1999 | Am J Perinatol1999，16，41： | 1 |
| 8 | Beta-blockers for cardiac CT:a primer for the radiologist 显示文摘 | Pannu HK Alvarez W Jr Fishman EK | 2006 | A JR Am J Roentgenol2006，186，62： | 1 |
| 9 | Functional impairment of bone formation in the pathogenesis of osteoporosis :the bone marrow regenerative competence显示文摘 | Bidwell JP Alvarez MB Hood Jr M | 2013 | Curr Osteoporos Rep2013，11，2： | 1 |
| 10 | Preclinical stud-ies and clinical utilization of monoclonal antibodies in epithelial o-varian cancer显示文摘 | Frederick PJ Straughn JM Jr Alvarez RD | 2009 | Gynecol Oncol2009，113，3： | 1 |
| 11 | Influence of microwave irradiation in the cyclohexane oxidation catalyzed by Fe (Ⅲ) complexes显示文摘 | Carvalho N M F Alvarez H M Jr A H | 2008 | Catal Today2008，，： | 1 |
| 12 | Early calcification of the aortic mitroflow pericardial bioprosthesis in the elderly显示文摘 | Alvarez JR Sierra J Vega M | | 0，，05： | 1 |
| 13 | 5-Hydroxytryptamine 5-HT2A receptor and 5-hydroxytryptamine transporter polymorphisms in acute myocardial infarction 显示文摘 | Coto E Reguero JR Alvarez V | 2003 | Clin Sci(Lond)2003，104，3： | 1 |
| 14 | Management of spontaneous congenital chylothorax: Oral medium -chain triglycerides versus total parenteral nutrition 显示文摘 | Femandez Alvarez JR Kalache KD GraVel EL | 1999 | Am J Perinatol1999，16，8： | 1 |
| 15 | Throbosponclin-1 expression in epithelial ovarian carcinoma: Association with p53 status, tumor angiogenesis, and survival in platinum-treated patients显示文摘 | Alvarez AA Axelrod JR Whitaker RS | 2001 | Gynecol Oncol2001，82，2： | 1 |
| 16 | Surface - en-hanced Raman scattering for ultrasensitive chemical analysis of 1 and2 - naphthalenethiols显示文摘 | Alvarez - Puebla R A Dos Santos D S Jr Aroca R F | 2004 | Analyst2004，129，12： | 1 |
| 17 | Duration of antimicrobial prophylaxis for group Bstreptococcus in patients with preterm premature of membranes who are not in labor显示文摘 | Alvarez JR Williams SF Ganesh VL | 2007 | Am J of Obstet Gynecol2007，197，4： | 1 |
| 18 | lack of association between polymorphisms of the coagulation factor VII and myocardial infarction in middle - aged Spanish men 显示文摘 | Batalla A Alvarez R Reguero JR | 2001 | lnt - J - Cardiol2001，80，23： | 1 |
| 19 | Duration of antimicrobial prophylaxis for group B streptococcus in patients with preterm premature rupture of membranes who are not in labor显示文摘 | Alvarez JR Williams SF Ganesh VL | 2007 | Am J of Obstet Gynecol2007，197，4： | 1 |
| 20 | Preoperative statin treatment reduces systemic inflam- matory response and myocardial damage in cardiac sur- gery显示文摘 | Martinez-Comendador JM Alvarez JR Mosquera I | 2009 | Eur J Cardiothorac Surg2009，36，6： | 1 |
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