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| 1 | 5种中国苏云金芽孢杆菌的伴孢晶体蛋白基因分析显示文摘利用聚合酶联反应 (PCR)和聚丙烯酰胺凝胶电泳 (SDS PAGE)技术分析了 5种中国苏云金杆菌制剂菌株的伴孢晶体蛋白及其基因组成。结果发现 ,5种菌株均含有cry1Aa和 /或c和 /或d和 /或b基因 ,只有Bt+Virus菌株含有cry1Ab基因 ,cry1A基因编码的伴孢晶体蛋白分子量约为 130kD ;仅有JS BtC菌株含有cry1B基因 ,其编码的伴孢晶体蛋白分子量约为 138kD ;除HB BtC菌株外 ,其余 4个菌株均含有cry2Aa和 /或b基因 ,这类基因编码分子量为 70kD的伴孢晶体蛋白 ;所有 5个菌株都含有cry1I基因 ,其编码的伴孢晶体蛋白分子量应为 81 2kD ,但实验中未曾检测到cry1I基因的表达 ; | 张光美 刘树生 Akhurst Raymond | 2000 | 昆虫学报2000，43，2： | 6 |
| 2 | Phase variation in Xenorhabdus nematophilus and Photorhabdus luminescens: differences in respiratory activity and membrane energization显示文摘 | RAYMOND J AKHURST NOEL E BOEMARE | 1994 | Applied and Environmental Microbiology1994，60，1： | 1 |
| 3 | Prospective evaluation of ^(18)F-FDG positron emission tomography in the preoperative staging of patients with hepatic colorectal metastases显示文摘Background:Despite considerable advances in preoperative imaging,up to one-third of patients operatively explored for hepatic colorectal metastases are unexpectedly found to harbor unresectable intrahepatic or extrahepatic disease.Methods:The current study is a prospective,blinded study comparing utility of[18F]2-fluoro-2-deoxyglucose positron emission tomography(18F-FDG-PET)to computed tomography(CT)and CT arterial portography(CTAP)as preoperative staging.Results:The 125 planned subjects were enrolled.Findings seen on FDG-PET alone changed therapy for 23 of 125 patients(18%).FDG-PET confirmed other radiologic findings in 16 cases(13%),for an overall influence on therapy in 39 cases(31%).FDG-PET was the most sensitive diagnostic imaging test for extrahepatic cancer;it was 80-90%sensitive for extrahepatic cancer and 70-90%specific.For the 28 cases of unresectable disease due to extrahepatic disease,FDG-PET findings solely changed therapies in 16 cases(57%)and influenced therapy in seven other cases(25%).Of the 21 unresectable cases due to extent of intrahepatic disease,FDG-PET did not solely change therapy in any.Overall,FDG-PET had the lowest sensitivity for hepatic sites compared with CT or CTAP.In particular,small(<1 cm)liver tumors were particularly poorly detected by FDG-PET.The area under the receiver operating characteristic(ROC)curve for small tumors was 0.58 and for patients on chemotherapy it was 0.66,a modest improvement over no imaging.Conclusions:FDG-PET is an important test for preoperative staging of patients with hepatic colorectal metastases,affecting treatment decisions in nearly one-third of patients.The high yield is due mainly to detection of extrahepatic disease.It is therefore recommended in patients with extrahepatic lesions suspected to be disseminated cancer or those with high risk for extrahepatic disease.It is not a good test for identification of small tumors in the liver. | Tim Akhurst Mithat Gönen Raymond E.Baser Lawrence H.Schwartz Scott Tuorto Lynn A.Brody Anne Covey Karen T.Brown Steven M.Larson Yuman Fong | 2022 | Hepatobiliary Surgery and Nutrition2022，11，4： | 1 |
| 4 | Identification and characterization of proteins from Bacillus thuringiensis with high toxic activity against the sheep blowfly, Lucilia cuprina显示文摘 | Joanne M. Gough David H. Kemp Raymond J. Akhurst Roger D. Pearson Kritaya Kongsuwan | 2005 | Journal of Invertebrate Pathology2005，，1： | 1 |
| 5 | Unusually High Frequency of Genes Encoding Vegetative Insecticidal Proteins in an Australian Bacillus thuringiensis Collection显示文摘 | Cheryl E. Beard Leon Court Annemie Boets Roslyn Mourant Jeroen Rie Raymond J. Akhurst | 2008 | Current Microbiology2008，，3： | 1 |
| 6 | Binding of Cry δ-endotoxins to brush border membrane vesicles of Helicoverpa armigera and Helicoverpa punctigera (Lepidoptera: Noctuidae)显示文摘到 Cry1Ac 的 Helicoverpa 有佩带徽章权利的人 a 的相对低的危险性,它到在在转基因的棉花的 Cry1Ac 的表示的化学杀虫药剂和季节的衰落的抵抗的历史要求了表示二杀虫的蛋白质提供这个多民族的害虫的持续控制的棉花的开发。管理抵抗问题,第二杀虫的蛋白质有行动 toCry1Ac 的一个显著地不同的模式,是必要的。到在象 H 一样的几种的 Cry1A 蛋白质的抵抗的一个普通特征。有佩带徽章权利的人啊哈是在有约束力的地点的一个变化。为在刷子的一些叫喊蛋白质的有约束力的地点的研究毗邻 H 的膜泡(BBMV ) 。有佩带徽章权利的人 and Helicoverpa punctigera 被承担。为 Cry1Ac 的 Thebinding 亲密关系比为 Cry1Ab 高,匹配他们毒性, andCry1Ac 和 Cry1Ab 被发现分享在两 H 的至少一个有约束力的地点的亲戚。有佩带徽章权利的人 and H.punctigera。然而, Cry2Aa 没为绑定与 Cry1Ac 竞争能那么与 Cry1Ac 在联合在转基因的棉花被使用控制 H。有佩带徽章权利的人一 and 管理抵抗。Variationin 三不同 H 的危险性。有佩带徽章权利的人一到 Cry1Ac 的紧张与参数 B_( 最大)/K_(com ) 相关。 | CHUNYAN LIAO LIZ BROOKS STEPHEN C. TROWELL RAYMOND J. AKHURST | 2005 | Insect Science2005，12，4： | 0 |
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