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| 1 | 初榨橄榄油中多酚化合物的UPLC-FLD检测及其抗氧化活性研究显示文摘采用超高效液相色谱荧光检测法(UPLC-FLD)检测不同品种初榨橄榄油中6种多酚化合物的含量,并测定样品的总多酚含量及抗氧化活性,同时对多酚组分与抗氧化能力的相关性进行分析。结果表明:色谱条件为采用Cadenza色谱柱(150 mm×4.6 mm,5μm),柱温35℃,以0.1%甲酸-水(A)-乙腈(B)为流动相进行梯度洗脱,荧光光谱激发波长为278 nm,发射波长为339 nm。在此条件下,初榨橄榄油样品中6种多酚化合物可在17 min内得到较好分离,并在较宽的浓度范围内呈良好的线性关系(R2≥0.9995),且精密度高,日内精密度相对标准偏差(RSD)≤4.91%,日间精密度RSD≤5.41%,加标回收结果准确可靠,平均回收率93.42%~119.86%,RSD≤6.26%。不同初榨橄榄油样品中羟基酪醇、酪醇、香草酸、松脂酚及总多酚含量差异较大,而橄榄苦苷和女贞苷含量最低,且样品间差异较小。初榨橄榄油多酚提取物具有良好的抗氧化活性,但不同品种间具有较大差异,ABTS法测定的总抗氧化能力为0.95~2.71 mmol·L^(-1) Trolox,DPPH自由基清除率为7.22%~66.23%,抑制羟自由基能力为13.24~136.06 U·mL^(-1),其中Coratina的抗氧化活性最强,总多酚含量与提取物总抗氧化能力呈显著正相关。 | 李雪 张玉 王君虹 朱作艺 孙素玲 刘志 朱申龙 沈国新 Agusti ROMERO 王伟 | 2021 | 浙江农业学报2021，33，5： | 3 |
| 2 | Thrombolysis-Related Hemorrhagic Infarction: A Marker of Early Reperfusion, Reduced Infarct Size, and Improved Outcome in Patients With Proximal Middle Cerebral Artery Occlusion显示文摘 | Carlos A. Molina José Alvarez-Sabín Joan Montaner Sonia Abilleira Juan F. Arenillas Pilar Coscojuela Francisco Romero Agusti Codina | 2002 | Stroke: Journal of the American Heart Association2002，，： | 1 |
| 3 | Pedigree analysis of 220 almond genotypes reveals two world mainstream breeding lines based on only three different cultivars显示文摘Loss of genetic variability is an increasing challenge in tree breeding programs due to the repeated use of a reduced number of founder genotypes.However,in almond,little is known about the genetic variability in current breeding stocks,although several cases of inbreeding depression have been reported.To gain insights into the genetic structure in modern breeding programs worldwide,marker-verified pedigree data of 220 almond cultivars and breeding selections were analyzed.Inbreeding coefficients,pairwise relatedness,and genetic contribution were calculated for these genotypes.The results reveal two mainstream breeding lines based on three cultivars:“Tuono”,“Cristomorto”,and“Nonpareil”.Descendants from“Tuono”or“Cristomorto”number 76(sharing 34 descendants),while“Nonpareil”has 71 descendants.The mean inbreeding coefficient of the analyzed genotypes was 0.041,with 14 genotypes presenting a high inbreeding coefficient,over 0.250.Breeding programs from France,the USA,and Spain showed inbreeding coefficients of 0.075,0.070,and 0.037,respectively.According to their genetic contribution,modern cultivars from Israel,France,the USA,Spain,and Australia trace back to a maximum of six main founding genotypes.Among the group of 65 genotypes carrying the S f allele for self-compatibility,the mean relatedness coefficient was 0.125,with“Tuono”as the main founding genotype(24.7%of total genetic contribution).The results broaden our understanding about the tendencies followed in almond breeding over the last 50 years and will have a large impact into breeding decision-making process worldwide.Increasing current genetic variability is required in almond breeding programs to assure genetic gain and continuing breeding progress. | Felipe Perez de los Cobos Pedro J.Martinez-Garcia Agusti Romero Xavier Miarnau Iban Eduardo Werner Howad Mourad Mnejja Federico Dicenta Rafel Socias i Company Maria J.Rubio-Cabetas Thomas M.Gradziel Michelle Wirthensohn Henri Duval Doron Holland Pere Arus Francisco J.Vargas Ignasi Batlle | 2021 | Horticulture Research2021，8，1： | 0 |
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