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| 1 | Lowered HGK expression inhibits cell invasion and adhesion in hepatocellular carcinoma cell line HepG2显示文摘AIM:To investigate the effects of RNA interference tar-geting hepatocyte progenitor kinase-like kinase(HGK) in the invasion and adhesion of hepatocellular carcinoma(HCC) cell line HepG2.METHODS:Three paired insert DNA fragments specif ic to HGK gene and one negative control DNA fragment were synthesized and inserted into RNAi-Ready pSIREN-RetroQ-ZsGreen vector.Western blotting assay and real-time reverse transcriptase polymerase chain reaction(RT-PCR) were used to screen the vector with a highest inhibitory rate.The vector was used to generate recom-binant retrovirus specif ic to HGK.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide(MTT) assay was used to examine cell growth;wound closure assay and cell adhesion assay were employed to investigate cell migration and adhesion respectively;and transwell assay and three-dimensional culture invasion assay were used to detect cell invasion.The expressions of matrix metalloproteinase(MMP)-2,MMP-9 and nuclear factor(NF)-κB were detected by Western blotting assay.RESULTS:The real time RT-PCR and Western blotting assay showed that cells transfected with retrovirus me-diating RNAi targeting of HGK(RV-shHGK)-1 vector had the strongest inhibition of HGK protein,with an inhibi-tion rate of 76%,and this vector was used to generate recombinant retrovirus RV-shHGK-1.Cell adhesion assay and MTT assay found that cell adhesion and growth of the cells infected with RV-shHGK-1 were significantly lower than those of the control cells(P < 0.05).Wound closure assay,transwell assay and three-dimensional culture invasion assay showed that the cell invasiveness was significantly less in HGK knockdown cells than in the control cells(P < 0.05).The expressions of MMP-2,MMP-9 and NF-κB were inhibited in HepG2 cells infected with RV-shHGK-1.CONCLUSION:Down-regulation of HGK can obviously inhibit the migration and invasion of HepG2 cells in vitro.HGK may be a new therapeutic target for treatment of HCC. | Su-Xia Han,Qing Zhu,Jin-Lu Ma,Jing Zhao,Xi Jia,Dan Zhang,Oncology Center of the First Aff iliated Hospital,College of Medicine,Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaChen Huang,Central Laboratory,College of Medicine,Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China | 2010 | World Journal of Gastroenterology2010，16，36： | 3 |
| 2 | Decreased IgA+ plasma cells and IgA expression in acute liver necrosis mice显示文摘AIM: To investigate the number of intestinal immunoglobulin A (IgA+) plasma cells and expression of intestinal IgA in mice with acute liver necrosis. METHODS: A model of acute liver necrosis was established by intraperitoneal injection of galactosamine (GalN) and lipopolysaccharide (LPS). Sixty mice were randomly divided into one of 4 equal groups: normal control, acute liver necrosis, LPS, or GalN. Hematoxylin and eosin staining, immunohistochemistry, and an enzyme-linked immunosorbent assay were employed to assess liver and intestinal injury, count intestinal IgA+ plasma cells, and measure the expression level of IgA and interferon γ (IFN-γ) in the small intestinal mucosa of mice. RESULTS: Injured intestinal mucosa was observed in the acute liver necrosis group but not in the normal, LPS or GalN groups. Compared with the normal group,intestinal IgA+ plasma cells were slightly decreased in the LPS and GalN groups [429 ± 20 per high power f ield (HPF), 406 ± 18/HPF, respectively], whereas they were markedly decreased in the acute liver necrosis group (282 ± 17/HPF vs 495 ± 26/HPF in normal group, P < 0.05). The expression of intestinal IgA was also slightly decreased in LPS and GalN groups, but was markedly reduced in the acute liver necrosis group as determined by enzyme-linked immunosorbent assay (P < 0.05). In contrast, the level of IFN-γ was slightly increased in LPS, GalN and acute liver necrosis groups, but with no statistical signif icance (P > 0.05). CONCLUSION: Intestinal IgA+ plasma cells and IgA expression levels indicating that mucosal immune barrier dysfunction, does exist in acute liver necrosis. | Jin-Long Fu, Zhao-Han Wang, Guo-Zhen Li, Yu-Rong Wang, Pei Liu, Department of Infectious Diseases, The First Aff iliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China | 2010 | World Journal of Gastroenterology2010，16，30： | 3 |
| 3 | The lithiasic acute cholecystitis:laparoscopic treatment (about106cases)显示文摘 | Beyrouti M I Beyrouti R Affes N | | 0，，04： | 1 |
| 4 | Arthroplasty versusinternal fixation for femoral neck fractures in the elderly 显示文摘 | Nicolaides V Galanakos S Mavrogenis AFf | 2011 | Strate-gies Trauma Limb Reconstr2011，1，： | 1 |
| 5 | A Maximum Like- lihood Time Delay Estimation in a Multipath Environment Using Importance Sampling 显示文摘 | Masmoudi A Bellili F Affes S | 2013 | IEEE Transactions onSignal Processing2013，61，1： | 1 |
| 6 | Moment-based SNR estimation over linearly-modulated wireless SIMO channels 显示文摘 | Stephenne A Bellili F Affes S | 2010 | IEEE Transactions on Wireless Communications2010，9，2： | 1 |
| 7 | Regulating transgenic crops:a comparative analysis of different regulatory processes显示文摘 | Gregory J affe | 2004 | Transgenic Research2004，13，： | 1 |
| 8 | A study of the LCMV and MVDR noise reduction filters显示文摘 | SOUDEN M BENESTY J AFFES S | | 0，，09： | 1 |
| 9 | Moment-based SNR estimation over linearly-modulated wireless SIMO channels显示文摘 | STEPHENNE A BELLILI F AFFES S | 2010 | IEEE Transactions on Wireless Communications2010，9，2： | 1 |
| 10 | A New OFDM Synchronization Symbol for Carrier Frequency Offset Estimation 显示文摘 | LAOURINE A STEPHENNE A AFFES S | 2007 | IEEE Signal Processing Letters2007，14，5： | 1 |
| 11 | Histopathological findings in appendectomy specimens:a study of 24,697 cases显示文摘 | CHARFI S SELLAMI A AFFES A | 2014 | Int J Colorectal Dis2014，29，8： | 1 |
| 12 | Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process显示文摘 | PalatsiJ Affes R Fernandez B | 2012 | Water Research2012，46，16： | 1 |
| 13 | Geographic location of knowledge spillovers as evidenced by patent citations 显示文摘 | AFFE A TRAJTENBERGM HENDERSON R | 1993 | Quarterly Journal of Economic1993，108，3： | 1 |
| 14 | Moment-based SNR Estimation over Linearly-Modulated Wireless SIMO Channels显示文摘 | STEPHENNE A BELLILI F AFFES S | 2010 | Wireless Communications IEEE Transac- tions on2010，9，02： | 1 |
| 15 | Fractional-delay sequen- tial blind beamforming for wireless multipath communications in con- fined areas显示文摘 | FARES S A DENIDNI T A AFFES S | 2008 | IEEE Transactions on Wireless Communications2008，7，2： | 1 |
| 16 | Broadband source localization from aneigenanalysis perspective显示文摘 | Souden M Benesty J Affes S | 2010 | IEEE Transactions on Audio Speech and Language Processing2010，18，6： | 1 |
| 17 | Low - Cost Trans- ceiver Architectures for 60 GHz Ultra Wideband WLANs 显示文摘 | S O Tatu E Moldovan S Affes | 2009 | International Journal of Digital Mul- timedia Broadcasting2009，，： | 1 |
| 18 | Hydrotreatment of bitumen from the whiterocks oil sand deposit显示文摘 | Longst Aff D C Deo M D H Anson F V | | 0，，09： | 1 |
| 19 | A generalized steered response power method for computationally viable source localization显示文摘 | Dmochowski J P Benesty J Affes S | | 0，，08： | 1 |
| 20 | Influence of Ad- sorption and Anaerobic Granular Sludge Characteris- tics on Long Chain Fatty Acids Inhibition Process显示文摘 | Palatsi J Affes R Fernandez | 2012 | Water Research2012，46，16： | 1 |
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