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您的检索式：作者名="Adrian D.Hegeman"
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| 1 | Genetic analysis of stilbenoid profiles in grapevine stems reveals a major mQTL hotspot on chromosome 18 associated with disease-resistance motifs显示文摘Grapevine(Vitis spp.)contains a wealth of phytochemicals that have received considerable attention due to healthpromoting properties and biological activities as phytoalexins.To date,the genetic basis of the quantitative variations for these potentially beneficial compounds has been limited.Here,metabolic quantitative trait locus(mQTL)mapping was conducted using grapevine stems of a segregating F2 population.Metabolic profiling of grapevine stems was performed using liquid chromatography–high-resolution mass spectrometry(LC-HRMS),resulting in the detection of 1317 ions/features.In total,19 of these features matched with literature-reported stilbenoid masses and were genetically mapped using a 1449-SNP linkage map and R/qtl software,resulting in the identification of four mQTLs.Two large-effect mQTLs that corresponded to a stilbenoid dimer and a trimer were mapped on chromosome 18,accounting for phenotypic variances of 29.0%and 38.4%.Functional annotations of these large-effect mQTLs on the VitisNet network database revealed a major hotspot of disease-resistance motifs on chromosome 18.This 2.8-Mbp region contains 48 genes with R-gene motifs,including variants of TIR,NBS,and LRR,that might potentially confer resistance to powdery mildew,downy mildew,or other pathogens.The locus also encompasses genes associated with flavonoid and biosynthetic pathways that are likely involved in the production of secondary metabolites,including phytoalexins.In addition,haplotype dosage effects of the five mQTLs further characterized the genomic regions for differential production of stilbenoids that can be applied in resistance breeding through manipulation of stilbenoid production in planta. | Soon L.Teh Bety Rostandy Mani Awale James J.Luby Anne Fennell Adrian D.Hegeman | 2019 | Horticulture Research2019，6，1： | 1 |
| 2 | Metabolic signatures of Arabidopsis thaliana abiotic stress responses elucidate patterns in stress priming,acclimation,and recovery显示文摘Temperature,water,and light are three abiotic stress factors that have major influences on plant growth,development,and reproduction.Plants can be primed by a prior mild stress to enhance their resistance to future stress.We used an untargeted metabolomics approach to examine Arabidopsis thaliana 11-day-old seedling’s abiotic stress responses including heat(with and without priming),cold(with and without priming),water-deficit and high-light before and after a 2-day-recovery period.Analysis of the physiological phenotypes showed that seedlings with stress treatment resulted in a reduction in fresh weight,hypocotyl and root length but remained viable.Several stress responsive metabolites were identified,confirmed with reference standards,quantified,and clustered.We identified shared and specific stress signatures for cold,heat,water-deficit,and high-light treatments.Central metabolism including amino acid metabolism,sugar metabolism,glycolysis,TCA cycle,GABA shunt,glutathione metabolism,purine metabolism,and urea cycle were found to undergo changes that are fundamentally different,although some shared commonalities in response to different treatments.Large increases in cysteine abundance and decreases in reduced glutathione were observed following multiple stress treatments highlighting the importance of oxidative stress as a general phenomenon in abiotic stress.Large fold increases in low-turnover amino acids and maltose demonstrate the critical role of protein and starch autolysis in early abiotic stress responses. | Yuan Xu Dana M.Freund Adrian D.Hegeman Jerry D.Cohen | 2022 | Stress Biology2022，2，1： | 0 |
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