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您的检索式：作者名="AZMAT Muhammad Abubakkar"
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| 1 | Comprehensive screening and selection of okra (Abelmoschus esculentus) germplasm for salinity tolerance at the seedling stage and during plant ontogeny显示文摘The okra germplasm was screened for salinity tolerance at the seedling stage and during plant ontogeny.Substantial variation existed in okra for salinity tolerance at the seedling stage.An 80 mmol/L NaCl concentration was suitable for discriminating tolerant and non-tolerant okra genotypes.The pooled ranking of the genotypes,based on individual rankings for each trait(root and shoot length,germination percentage,and relative Na+ and K+)in individual NaCl concentrations,was effective for selecting tolerant genotypes.Genotypes selected at the seedling stage maintained their tolerance to NaCl during plant ontogeny,suggesting that screening of the germplasm entries and advanced breeding materials for salt tolerance at the seedling stage is effective.Among 39 okra genotypes,five were identified as the most tolerant genotypes and showed potential for use in breeding programs that focus on the development of salt-tolerant,high-yield okra cultivars. | Ikram-ul-HAQ Asif Ali KHAN Iqrar Ahmad KHAN Muhammad Abubakkar AZMAT | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，7： | 4 |
| 2 | Extraction of DNA suitable for PCR applications from mature leaves of Mangifera indica L.显示文摘Good quality deoxyribonucleic acid (DNA) is the pre-requisite for its downstream applications. The presence of high concentrations of polysaccharides, polyphenols, proteins, and other secondary me- tabolites in mango leaves poses problem in getting good quality DNA fit for polymerase chain reaction (PCR) applications. The problem is exacerbated when DNA is extracted from mature mango leaves. A reliable and modified protocol based on the cetyl- trimethylammonium bromide (CTAB) method for DNA extraction from mature mango leaves is described here. High concentrations of inert salt were used to remove polysaccharides; Polyvinylpyrrolidone (PVP) and β-mercaptoethanol were employed to manage phenolic compounds. Extended chloroform-isoamyl alcohol treatment followed by RNase treatment yielded 950?1050 μg of good quality DNA, free of protein and RNA. The problems of DNA degradation,contamination, and low yield due to irreversible binding of phenolic compounds and coprecipitation of polysaccharides with DNA were avoided by this method. The DNA isolated by the modified method showed good PCR amplification using simple se- quence repeat (SSR) primers. This modified protocol can also be used to extract DNA from other woody plants having similar problems. | Muhammad Abubakkar AZMAT Iqrar Ahmad KHAN Hafiza Masooma Naseer CHEEMA Ishtiaq Ahmad RAJWANA Ahmad Sattar KHAN Asif Ali KHAN | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，4： | 1 |
| 3 | Comparative analysis of SIMILAR to RCD ONE(SRO)family from tetraploid cotton species and their diploid progenitors depict their significance in cotton growth and development显示文摘Background SRO(Similar to RCD1)genes family is largely recognized for their importance in the growth,develop-ment,and in responding to environmental stresses.However,genome-wide identification and functional characteri-zation of SRO genes from cotton species have not been reported so far.Results A total of 36 SRO genes were identified from four cotton species.Phylogenetic analysis divided these genes into three groups with distinct structure.Syntenic and chromosomal distribution analysis indicated uneven distribu-tion of GaSRO,GrSRO,GhSRO,and GbSRO genes on A2,D5 genomes,Gh-At,Gh-Dt,Gb-At,and Gb-Dt subgenomes,respectively.Gene duplication analysis revealed the presence of six duplicated gene pairs among GhSRO genes.In promoter analysis,several elements responsive to the growth,development and hormones were found in GhSRO genes,implying gene induction during cotton growth and development.Several miRNAs responsive to plant growth and abiotic stress were predicted to target 12 GhSRO genes.Organ-specific expression profiling demonstrated the roles of GhSRO genes in one or more tissues.In addition,specific expression pattern of some GhSRO genes dur-ing ovule development depicted their involvement in these developmental processes.Conclusion The data presented in this report laid a foundation for understanding the classification and functions of SRO genes in cotton. | SHABAN Muhammad TABASSUM Riaz RANA Iqrar Ahmad ATIF Rana Muhammad AZMAT Muhammad Abubakkar IQBAL Zubair MAJEED Sajid AZHAR Muhammad Tehseen | 2024 | Journal of Cotton Research2024，7，1： | 0 |
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