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| 1 | Skin bioprinting:the future of burn wound reconstruction?显示文摘Burns are a significant cause of trauma,and over the years,the focus of patient care has shifted from just survival to facilitation of improved functional outcomes.Typically,burn treatment,especially in the case of extensive burn injuries,involves surgical excision of injured skin and reconstruction of the burn injury with the aid of skin substitutes.Conventional skin substitutes do not contain all skin cell types and do not facilitate recapitulation of native skin physiology.Three-dimensional(3D)bioprinting for reconstruction of burn injuries involves layer-by-layer deposition of cells along with scaffolding materials over the injured areas.Skin bioprinting can be done either in situ or in vitro.Both these approaches are similar except for the site of printing and tissue maturation.There are technological and regulatory challenges that need to be overcome for clinical translation of bioprinted skin for burn reconstruction.However,the use of bioprinting for skin reconstruction following burns is promising;bioprinting will enable accurate placement of cell types and precise and reproducible fabrication of constructs to replace the injured or damaged sites.Overall,3D bioprinting is a very transformative technology,and its use for wound reconstruction will lead to a paradigm shift in patient outcomes.In this review,we aim to introduce bioprinting,the different stages involved,in vitro and in vivo skin bioprinting,and the various clinical and regulatory challenges in adoption of this technology. | Mathew Varkey Dafydd O.Visscher Paul P.Mvan Zuijlen Anthony Atala James J.Yoo | 2019 | Burns & Trauma2019，7，1： | 8 |
| 2 | Decellularization for whole organ bioengineering显示文摘 | J E Arenas-Herrera I K Ko A Atala J J Yoo | 2013 | Biomedical Materials2013，，1： | 2 |
| 3 | Tissue specific synthetic ECM hydrogels for 3-D in vitro maintenance of hepatocyte function显示文摘 | Aleksander Skardal Leona Smith Shantaram Bharadwaj Anthony Atala Shay Soker Yuanyuan Zhang | 2012 | Biomaterials2012，，18： | 2 |
| 4 | The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction显示文摘 | Bryan W. Tillman Saami K. Yazdani Sang Jin Lee Randolph L. Geary Anthony Atala James J. Yoo | 2008 | Biomaterials2008，，4： | 2 |
| 5 | Endoscopic treatment of vesicoureteral reflux with a chondrocyte-alginate suspension显示文摘 | Atala A Kim W Paige KT | 1994 | Urol1994，152，： | 1 |
| 6 | Tissue engineering,stem cells,cloning, and parthenogenesis:new paradigms for therapy 显示文摘 | Hipp J Atala A | 2004 | J Exp Clin Assist Reprod2004，1，1： | 1 |
| 7 | Biomaterials for Tissue Engineering显示文摘 | Kim BS Baze CE Atala A | 2000 | Word J Urology2000，8，1： | 1 |
| 8 | Bilayered scaffold for engineering eellutarized blood vessels显示文摘 | J u Y M Choi J S Atala A | 2010 | Biomaterials2010，31，15： | 1 |
| 9 | Systems for therapeutic angiogenesis in tissue engineering显示文摘 | Soker S Machado M Atala A | 2000 | World J Urol2000，18，： | 1 |
| 10 | Engineering of vaginal tissue in vivo显示文摘 | De Filippo RE Yoo JJ Atala A | 2003 | Tissue Eng2003，9，2： | 1 |
| 11 | A novel intercollagen matrix forhypospadias repair显示文摘 | Atala A Guzman L Retik A | 1999 | J Urol1999，162，： | 1 |
| 12 | Continous release of endosta- tin form microencapasulated engineered cells for tumor therapy 显示文摘 | Joki T Machluf M Atala A | 2001 | Nat Biotechnol2001，19，1： | 1 |
| 13 | Tissue engineering, stem cells, cloning, and partheno- genesis:new paradigms for therapy显示文摘 | ttipp J Atala A | 2004 | J Exp Clin Assist Reprod2004，1，1： | 1 |
| 14 | Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion显示文摘 | Aase Bodin Shantaram Bharadwaj Shaofeng Wu Paul Gatenholm Anthony Atala Yuanyuan Zhang | 2010 | Biomaterials2010，，： | 1 |
| 15 | Carbon nanotubes applications for tissue engineering 显示文摘 | B S Harrison A Atala | 2007 | Biomaterials2007，28，： | 1 |
| 16 | Autologous penile corpora cavernosa replacement using tissue engineering techniques显示文摘 | Kwon TG Yoo JJ Atala A | 2002 | J Urol2002，168，42： | 1 |
| 17 | Proximal hypospadias显示文摘 | Cilento BG Atala A | 2002 | Urol Clin North Am2002，29，： | 1 |
| 18 | Tissue-engineered autologous bladders for patients needingcystoplasty显示文摘 | ATALA A BAUER S B SOKER S | 2006 | Lancet2006，367，9518： | 1 |
| 19 | In vivo evaluation of acellular human dermis for abdominal wall repair显示文摘 | Eberli D Rodriguez S Atala A | 2010 | J Biomed Mater Res A2010，93，4： | 1 |
| 20 | Amniotic fluid-derived stem cells in regenerative medicine research 显示文摘 | Joo S Ko IK Atala A | 2012 | Arch Pharm Res2012，35，： | 1 |
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