|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Study of the effect of atorvastatin on the interaction between ICAM-1 and CD11b by live-cell single-molecule force spectroscopy显示文摘The interaction between the cell adhesion molecule CD11b and its ligand ICAM-1 plays an important role in inflammatory responses in the disease of atherosclerosis. Atorvastatin is a commonly prescribed statin drug which has been considered as one of the most potent therapeutic agents for atherosclerosis due to its lipid-lowering effect. Recently, there is a growing body of evidence that atorvastatin has anti-inflammatory effect. We have applied the advanced method of live-cell single-molecule force spectroscopy to investigate the effect of atorvastatin on adhesion force between ICAM-1 and CD11b. Our result showed that single-molecule binding force of ICAM-1 and CD11b detected by AFM in the living cells was about 40 pN, and atorvastatin did not affect this force by blocking ICAM-1 or CD11b. This was different from the ICAM-1 monoclonal antibody, which could directly reduce the binding force of ICAM-1 and CD11b. Flow cytometry results revealed that atorvastatin pretreatment decreased the ICAM-1 expression in TNF-α activated HUVECs, which may contribute to its anti-inflammatory effect. The study provides a new approach to study anti-inflammatory mechanism for clinic drugs. | LI Yi1, SHI XiaoLi2, LIU HuiLiang1, YI ShaoQiong3, ZHANG XueJie2 & FANG XiaoHong2 1Department of Cardiology, the General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China 2Beijing National Laboratory for Molecular Sciences Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China 3State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China | 2010 | Science China Chemistry2010，53，4： | 2 |
| 2 | Studies on Human Fetal Lens Crystallins Under Oxidative Stress and Protective Effects of Tea Polyphenols显示文摘Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4 and H2O2, after 5 or 24 hours, the crystallins were analysed with SDS-PAGE and IEF.Results : There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control group.Conclusion: The results indicate that exposure of water-soluble | Hui Xiang, Suhua Pan, Shaozhen LiGuangdong Provincial Corps Hospital, Chinese People’ s Armed Police Forces, Guangzhou 510010, China Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China | 1998 | 眼科学报1998，14，3： | 2 |
| 3 | Synthesis and characterization of polypyrrole-magnetite-silica particles显示文摘 | Btterworth M D Bell S A Armes S P Simpson A W | 1996 | J Colloid Interf Sci1996，183，： | 1 |
| 4 | Preparation and characterization of polypyrrole-silica colloidal nanocomposites in water-methanol mixtures显示文摘 | Han M G Armes S P | 2003 | J Colloid Interface Sci2003，262，： | 1 |
| 5 | Affeetivecommitment to the organization: the contribution of per- ceived organizational support显示文摘 | RHOADES L EISENBERGER R ARMELI S | 2001 | Journal of applied psy- chology2001，86，5： | 1 |
| 6 | Recent advances in shell cross-linked mieelles显示文摘 | Read E S Armes S P | 2007 | Chem Commun2007，43，29： | 1 |
| 7 | Preparation and characterization of polypyrrole-silica colloidal nanocomposites in water-methanol mixtures显示文摘 | Han M G Armes S P | 2003 | J Colloid Interface Sci2003，262，： | 1 |
| 8 | Conducting polymer-colloidal silica composites 显示文摘 | Armes S P Gottesfeld S Berry J G | 1991 | Polymer1991，32，13： | 1 |
| 9 | Affective Commitment to the Organization :The Contribution of Perceived Organizational Support 显示文摘 | Rhoades L Eisenberger R Armeli S | 2001 | Journal of Applied Psychology2001，86，5： | 1 |
| 10 | Facile Atom Transfer Radical Polymerization of Methoxy Capped Oligo (ethylene glycol)Methacrylate in Aqueous Media at Ambient Temperature显示文摘 | Wang X S Armes S P | 2000 | Macromolecules2000，33，： | 1 |
| 11 | Conducting polymer colloidal silica composites显示文摘 | Armes S P Gottesfeld S Beery J G | 1991 | Polymer1991，32，： | 1 |
| 12 | Synthesis and aqueous solution properties of novel hydrophilic - hydrophilic block copolymers based on tertiary amine methacrylates 显示文摘 | BUTUN V BILLINGHAM N C ARMES S P | 1997 | Chem Commun1997，，7： | 1 |
| 13 | One-pot synthesis of biomimetic shell cross-linked micelles and nanocages by AT- RP in alcohol/water mixtures显示文摘 | Sugihara S Armes S P Lewis A L | 2010 | Angew Chem Int Ed2010，49，： | 1 |
| 14 | Conducting polymer-colloidal silicacomposites显示文摘 | Armes P Gottesfeld S Beery J G | 1991 | Polymer1991，32，： | 1 |
| 15 | 显示文摘 | Thompson K L Armes S P Howse J R | 2010 | Macromole- eules2010，43，10： | 1 |
| 16 | Reciprocation of Perceived Organizational Support显示文摘 | Eisenberger R Armeli S Rexwinkel B | 2001 | Journal of Applied Psychol- ogy2001，86，1： | 1 |
| 17 | Re ciprocation of perceived organizational support显示文摘 | EISENBERGER R ARMELI S REXWlNKEL B | 2001 | Journal of Applied Psychology2001，86，1： | 1 |
| 18 | Inorganic/organic hybrid materials:conducting polymer-silica nanocomposite particles显示文摘 | Maeda S GillM | 1993 | Polym Mater Sci Eng1993，70，： | 1 |
| 19 | Polypyrrole ~ tin (Ⅳ) oxide colloidal nanocomposites显示文摘 | Maeda S Armes S P | 1995 | Synth Met1995，69，： | 1 |
| 20 | Particle-size distri butions of polyaniline silica colloidal composites显示文摘 | Gill M Armes S P Fairhurst D | 1992 | Langmuir1992，8，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员