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| 1 | 多元PCR在黑鲍(Haliotis rubra)微卫星遗传研究中的应用显示文摘根据D03等11个微卫星引物单位点PCR扩增时优化的扩增条件(退火温度、MgCl2等)和11个微卫星引物所带有的荧光颜色、等位基因大小变异范围及反应的灵敏度,可将11个微卫星引物分成A组(D03、G04、B04和H08)、B组(A09、H11、A08和G01)和C组(CmrHr1.24、CmrHr2.30和CmrHr2.23)进行多元PCR扩增,结果显示各组内位点之间的分离清晰,这表明多元PCR可以大大提高微卫星检测仪器的使用效率和提高实验效果,在微卫星研究中是一种快速便捷的方法。 | 黎中宝 Appleyard Sharon A Elliott Nicholas G | 2005 | 海洋与湖沼2005，36，4： | 8 |
| 2 | 羊鲍(Haliotis ovina)和耳鲍(H.asinina)MtDNA COⅠ和COⅡ基因片段序列的比较研究显示文摘采用基因测序的方法,对我国海南产的羊鲍和耳鲍两个自然种群的COⅠ和COⅡ基因片段进行了PCR扩增和测序。结果表明,羊鲍和耳鲍COⅠ基因片段的核苷酸序列均为193bp,4种碱基组成非常相似,且其A+T的含量也非常相似,分别为45.08%和45.60%。羊鲍和耳鲍COⅠ基因片段的核苷酸序列之间有29bp的差异,其中有8处发生碱基颠换,21处发生碱基转换,差异为15.03%,同源性为84.97%;羊鲍和耳鲍COⅡ基因片段的核苷酸序列均为157bp,羊鲍和耳鲍4种碱基组成差异较大,且其A+T的含量也不同,分别为59.24%和55.41%。羊鲍和耳鲍COⅡ基因片段的核苷酸序列之间有19bp的差异,其中3处发生碱基颠换,16处发生碱基转换,达到12.10%的差异,同源性为87.90%。分子变异分析表明羊鲍和耳鲍物种间的变异组成为0.50,变异百分数为100.00,羊鲍和耳鲍物种间的遗传分化显著(FST=1,P<0.001)。 | 黎中宝 Appleyard Sharon A. Elliott Nicholas G. | 2008 | 海洋与湖沼2008，39，2： | 8 |
| 3 | Immunohistochemical expression of SP-NK-1R-EGFR pathway and VDR in colonic inflammation and neoplasia显示文摘AIM:To determine the expression of neurokinin-1receptor(NK-1R),phosphorylated epidermal growth factor receptor(p EGFR),cyclooxygenase-2(Cox-2),and vitamin D receptor(VDR)in normal,inflammatory bowel disease(IBD),and colorectal neoplasia tissues from Puerto Ricans.METHODS:Tissues from patients with IBD,colitisassociated colorectal cancer(CAC),sporadic dysplasia,and sporadic colorectal cancer(CRC),as well as normal controls,were identified at several centers in Puerto Rico.Archival formalin-fixed,paraffin-embedded tissues were de-identified and processed by immunohistochemistry for NK-1R,p EGFR,Cox-2,and VDR.Pictures of representative areas of each tissues diagnosis were taken and scored by three observers using a4-point scale that assessed intensity of staining.Tissues with CAC were further analyzed by photographing representative areas of IBD and the different grades of dysplasia,in addition to the areas of cancer,within each tissue.Differences in the average age between the five patient groups were assessed with one-way analysis of variance and Tukey-Kramer multiple comparisons test.The mean scores for normal tissues and tissues with IBD,dysplasia,CRC,and CAC were calculatedand statistically compared using one-way analysis of variance and Dunnett’s multiple comparisons test.Correlations between protein expression patterns were analyzed with the Pearson’s product-moment correlation coefficient.Data are presented as mean±SE.RESULTS:On average,patients with IBD were younger(34.60±5.81)than normal(63.20±6.13,P<0.01),sporadic dysplasia(68.80±4.42,P<0.01),sporadic cancer(74.80±4.91,P<0.001),and CAC(57.50±5.11,P<0.05)patients.NK-1R in cancer tissue(sporadic CRC,1.73±0.34;CAC,1.57±0.53)and sporadic dysplasia(2.00±0.45)were higher than in normal tissues(0.73±0.19).p EGFR was significantly increased in sporadic CRC(1.53±0.43)and CAC(2.25±0.47)when compared to normal tissue(0.07±0.25,P<0.05,P<0.001,respectively).Cox-2 was significantly increased in sporadic colorectal cancer(2.20±0.23 vs 0.80±0.37 for normal tissues,P<0.05).In comparison to normal(2.80±0.13)and CAC(2.50±0.33)tissues,VDR was significantly decreased in sporadic dysplasia(0.00±0.00,P<0.001 vs normal,P<0.001 vs CAC)and sporadic CRC(0.47±0.23,P<0.001 vs normal,P<0.001 vs CAC).VDR levels negatively correlated with NK-1R(r=-0.48)and p EGFR(r=-0.56)in normal,IBD,sporadic dysplasia and sporadic CRC tissue,but not in CAC.CONCLUSION:Immunohistochemical NK-1R and p EGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia.VDR immunoreactivity can distinguish CAC from sporadic cancer. | Raymond A Isidro Myrella L Cruz Angel A Isidro Axel Baez Axel Arroyo William A González-Marqués Carmen González-Keelan Esther A Torres Caroline B Appleyard | 2015 | World Journal of Gastroenterology2015，21，6： | 7 |
| 4 | Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease显示文摘AIM: To determine serum vitamin D levels and colonic vitamin D receptor(VDR) expression in inflammatory bowel disease(IBD) and non-IBD patients and correlate these with histopathology.METHODS: Puerto Rican IBD(n = 10) and non-IBD(n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis(active/inactive), and scored for the degree of inflammation present(0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.RESULTS: The IBD cohort was significantly younger(40.40 ± 5.27, P < 0.05) than the non-IBD cohort(56.70 ± 1.64) with a higher prevalence of vitamin D deficiency(40% vs 20%, respectively) and insufficiency(70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients(1.95 ± 0.25) than in normalappearing mucosa from control patients(0.25 ± 0.08, P < 0.01) and from IBD patients(0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients(r =-0.44, P < 0.05) and from IBD patients with Crohn's disease(r =-0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients(r = 0.38, P < 0.05) and with patient age(r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. | Yamilka Abreu-Delgado Raymond A Isidro Esther A Torres Alexandra González Myrella L Cruz Angel A Isidro Carmen I González-Keelan Priscilla Medero Caroline B Appleyard | 2016 | World Journal of Gastroenterology2016，22，13： | 5 |
| 5 | Characterization of cuttlebone for a biomimetic design of cellular structures显示文摘乌贼骨是拥有高孔,高曲折的僵硬和压缩力量的多功能的性质的自然材料,使它成为从本质上讲创造的细胞的结构的设计优化的一个好例子。乌贼骨使用扫描电子显微镜学(SEM ) 的检查揭示近似周期的微观结构,为用直接 homogenization 技术的计算描述适当。在这份报纸,体积部分和僵硬张肌基于二个不同单位房间模型被决定从二件不同墨鱼样品被提取。这些描绘的结果然后在试图与象乌贼骨的一样的性质改革微观结构的一个反的 homogenization 过程被用作目标价值。有到原来的乌贼骨单位房间的类似的拓扑学的单位房间被完成,达到一样的体积部分(即体积密度) 并且一样(或很靠近) 僵硬张肌。另外,交替的单位房间拓扑学的一个范围被完成也达到目标性质,揭示这个反的 homogenization 问题的非唯一的性质。关键词反的 homogenization - 乌贼骨微观结构 - 拓扑学优化 - 扫描电子显微镜学(SEM )- 额外光的 | Joseph Cadman Shiwei Zhou Yuhang Chen Wei Li Richard Appleyard Qing Li | 2010 | Acta Mechanica Sinica2010，26，1： | 3 |
| 6 | High Prevalence of Sessile Serrated Adenomas With BRAF Mutations: A Prospective Study of Patients Undergoing Colonoscopy显示文摘 | Kevin J. Spring Zhen Zhen Zhao Rozemary Karamatic Michael D. Walsh Vicki L.J. Whitehall Tanya Pike Lisa A. Simms Joanne Young Michael James Grant W. Montgomery Mark Appleyard David Hewett Kazutomo Togashi Jeremy R. Jass Barbara A. Leggett | 2006 | Gastroenterology2006，，5： | 3 |
| 7 | How does knowledge? Interfirm patterns in the semiconductor industry显示文摘 | Appleyard H | 1996 | Strategic Management Journal1996，17，： | 1 |
| 8 | Erlotinib inhibits progression to dysplasia in a colitis-associated colon cancer model显示文摘AIM:To investigate the role of epidermal growth factor receptor(EGFR) in colitis-associated dysplasia using the EGFR tyrosine kinase inhibitor erlotinib.METHODS:Sprague-Dawley rats received trinitrobenzene sulfonic acid(TNBS;30 mg in 50% ethanol,ic),followed 6 wk later by reactivation with TNBS(5 mg/kg,iv) for 3 d.To induce colitis-associated dysplasia,rats then received TNBS(iv) twice a week for 10 wk.One group received erlotinib(10 mg/kg,ip) for 1 wk before the start of the reactivation of the colitis and 2 wk after(21 d);the rest received the vehicle.After rats were euthanized,the colons were removed and analyzed for damage and expression of the EGFR downstream effectors Erk1/2 and c-Myc.RESULTS:Ninety percent of the vehicle-treated animals had dysplasia in any region of the colon.Erlotinib-treated animals had a significant decrease in the incidence of dysplasia compared to vehicle-treated animals in all regions of the colon(50.00% ± 11.47% vs 90.00% ± 10.00% in proximal,P < 0.05;15.00% ± 8.19% vs 50.00% ± 16.67% in mid,P < 0.05;and 20.00% ± 9.17% vs 70.00% ± 15.28% in distal,P < 0.01).Erlotinib-treated animals also had reduced cell proliferation,reduced active Erk1/2,and reduced c-Myc in colon epithelium compared with the vehicle-treated animals.In vitro,erlotinib treatment was shown to markedly decrease c-Myc and pErk1/2 levels in rat epithelial cells.Proliferation of rat epithelial cells was stimulated by epidermal growth factor and inhibited by erlotinib(P < 0.05).CONCLUSION:Erlotinib can decrease the development of colitis-associated dysplasia,suggesting a potential therapeutic use for erlotinib in patients with long-standing colitis. | Beatriz Pagán Angel A Isidro Myrella L Cruz Domenico Coppola Caroline B Appleyard | 2011 | World Journal of Gastroenterology2011，17，44： | 1 |
| 9 | Role of double balloon enteroscopy in obscure gastrointestinal bleeding显示文摘 | John S Appleyard M | 2008 | J Gastroenterol Hepatol2008，23，： | 1 |
| 10 | TrkB activation by brain-derived neurotrophic factor inhibits the Gprotein-gated inward rectifier Kir3 by tyrosine phosphorylation of the channel显示文摘 | Rogalski SL Appleyard SM Pattillo A | 2000 | J Biol Chem2000，275，25： | 1 |
| 11 | Haemochromatosisgenotype and iron overload:association with hypertension and leftventricular hypertrophy显示文摘 | Ellervik C Tybjaerg Hansen A Appleyard M | 2010 | J Int Med2010，268，3： | 1 |
| 12 | The stabilisation of carbon fibres studied by micro-thermal analysis显示文摘 | Blanco C Lu S Appleyard S P | 2003 | Carbon2003，41，1： | 1 |
| 13 | Hapten-induced chronic colitis in the rat: alternatives to trinitrobenzene sulfonic acid显示文摘 | Le T Carter L Appleyard CB Beck PL | 1995 | J Pharmacol Toxicol Methods1995，33，4： | 1 |
| 14 | Proopiomelanocortin neurons in nucleus tractus solitarius are activated by visceral afferents: regulation by cholecystokinin and opioids 显示文摘 | Appleyard SM Bailey TW Doyle MW | 2005 | Neuroseience2005，25，14： | 1 |
| 15 | Mycoplasma haemo felis and Mycoplasma haemominutum detection by polymerase chain reaction in cats from Saskatchewan and Alberta显示文摘 | KEWISH K E APPLEYARD G D MYERS S L | 2004 | Can Vet J2004，45，9： | 1 |
| 16 | Open innovation andstrategy 显示文摘 | CHESBROUGH H W APPLEYARD M M | 2007 | California Management Review2007，，50： | 1 |
| 17 | Stroke incidence and risk factors for stroke in Copenhagen, Denmark 显示文摘 | Boysen G Nyboe J Appleyard M | 1988 | Stroke1988，19，11： | 1 |
| 18 | Interaction effects in a one-dimensional constriction显示文摘 | THOMAS K J NICHOLLS J T APPLEYARD N J | 1998 | Physical Review B1998，58，8： | 1 |
| 19 | Smoking and the risk of the first acute myocardial infarction显示文摘 | NYBOE J JENSEN G APPLEYARD M | 1991 | Am Heart J1991，122，： | 1 |
| 20 | Oseoporosis influences the early period of fracture healing in a rat osteoporotie model显示文摘 | NAMKUNG-MATTHAI H APPLEYARD R JANSEN J | 2001 | Bone2001，28，1： | 1 |
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