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| 1 | Relationship between vitamin D and IL-23,IL-17 and macrophage chemoattractant protein-1 as markers of fibrosis in hepatitis C virus Egyptians显示文摘AIM:To assess vitamin D in hepatitis C patients and its relationship to interleukin (IL)-23, IL-17, and macrophage chemoattractant protein-1 (MCP-1). METHODS:The study was conducted on 50 Egyptian hepatitis C virus (HCV) genotype number IV-infected patients and 25 ageand gender-matched healthy subjects. Venous blood samples were obtained. Samples were allowed to clot and sera were separated by centrifugation and stored at -20 ℃. A 25 hydroxy vitamin D assay was carried out using solid phase RIA. A 1,25 dihydroxy vitamin D assay was carried out using a commercial kit purchased from Incstar Corporation. IL-17 and -23 and MCP-1 were assayed by an enzyme immunoassay. Quantitative and qualitative polymerase chain reaction for HCV virus were done by TaqMan technology. Only HCV genotype IV-infected subjectswere included in the study. The mean ± SD were determined, a t-test for comparison of means of different parameters was used. Correlation analysis was done using Pearson's correlation. Differences among different groups were determined using the Kruskal-Wallis test. RESULTS:The mean vitamin D level in HCV patients (groupⅠ) was 15 ± 5.2 ng/mL while in control (group Ⅱ) was 39.7 ± 10.8. For active vitamin D in groupⅠas 16.6 ± 4.8 ng/mL while in group Ⅱ was 41.9 ± 7.9. IL-23 was 154 ± 97.8 in group Ⅰ and 6.7 ± 2.17 in group Ⅱ. IL-17 was 70.7 ± 72.5 in cases and 1.2 ± 0.4 in control. MCP-1 was 1582 ± 794.4 in group Ⅰand 216.1 ± 5.38 in group Ⅱ. Vitamin D deficiency affected 72% of HCV-infected patients and 0% of the control group. Vitamin D insufficiency existed in 28% of HCV-in-fected patients and 12% of the control group. One hundred percent of the cirrhotic patients and 40% of non cirrhotic HCV-infected patients had vitamin D deficiency. IL-23, IL-17, and MCP-1 were markedly increased in HCV-infected patients in comparison to controls.A significant negative correlation between vitamin D and IL-17 and-23 and MCP-1 was detected. HCV-infected males and females showed no differences with respect to viral load, vitamin D levels, IL-17, IL-23 and MCP-1. The viral load was negatively correlated with vitamin D and active vitamin D (P = 0.0001 and P = 0.001, respectively), while positively correlated with IL-23, IL-17, and MCP-1. We classified the patients according to sonar findings into four groups. Group Ⅰa with bright hepatomegaly and included 14 patients. Group Ⅰb with perihepatic fibrosis and included 11 patients. Group Ⅰc with liver cirrhosis and included 11 patients. Group Ⅰd with he patocellular carcinoma (HCC) and included 14 patients. Vitamin D and active vitamin D were shown to be lower in cirrhotic patients and much lower in patients with HCC, and this difference was highly significant (P = 0.0001). IL-17 and-23 and MCP-1 were higher in advanced liver disease) and the differences were highly significant (P = 0.0001).CONCLUSION:Whether the deficiency of vitamin D is related to HCV-induced chronic liver disease or predisposing factor for higher viral load is a matter of debate. | Noha M El Husseiny Hala M Fahmy Waleed A Mohamed Hisham H Amin | 2012 | World Journal of Hepatology2012，4，8： | 12 |
| 2 | Vitamin D deficiency: Correlation to interleukin-17, interleukin-23 and PⅢNP in hepatitis C virus genotype 4显示文摘AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response mediators. METHODS: The study was conducted on 50 Egyptian patients (36 male, 14 female) with hepatitis C virus (HCV) infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 (HCV-4) infection (as new patients or under followup). Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma (HCC).Total Vit D (i.e., 25-OH-Vit D) and active Vit D [i.e., 1,25-(OH) 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS: Levels of Vit D and its active form were significantly lower in advanced liver disease (hepatic cirrhosis and/or carcinoma) patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP (r = -0.679, -0.801 and -0.920 at P < 0.001, respectively). HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlated with Vit D and active Vit D (r = -0.084 and -0.846 at P < 0.001, respectively), and positively correlated with IL-17, IL-23 and PⅢNP (r = 0.951, 0.922 and 0.94 at P < 0.001, respectively). Whether the deficiency in Vit D was related to HCVinduced chronic liver disease or was a predisposing factor for a higher viral load remains to be elucidated. CONCLUSION: The negative correlations between Vit D and IL-17, IL-23 and PⅢNP highlight their involvement in the immune response in patients with HCV-4related liver diseases in Egypt. | Mona F Schaalan Waleed A Mohamed Hesham H Amin | 2012 | World Journal of Gastroenterology2012，18，28： | 12 |
| 3 | Autoantibody profiles in autoimmune hepatitis and chronic hepatitis C identifies similarities in patients with severe disease显示文摘AIM To determine how the auto-antibodies(Abs) profiles overlap in chronic hepatitis C infection(CHC) and autoimmune hepatitis(AIH) and correlate to liver disease.METHODS Levels of antinuclear Ab, smooth muscle antibody(SMA) and liver/kidney microsomal-1(LKM-1) Ab and markers of liver damage were determined in the sera of 50 patients with CHC infection, 20 AIH patients and 20 healthy controls using enzyme linked immunosorbent assay and other immune assays. RESULTS We found that AIH patients had more severe liver disease as determined by elevation of total Ig G, alkaline phosphatase, total serum bilirubin and serum transaminases and significantly higher prevalence of the three non-organ-specific autoantibodies(auto-Abs) than CHC patients. Antinuclear Ab, SMA and LKM-1 Ab were also present in 36% of CHC patients and related to disease severity. CHC cases positive for auto-Abs were directly comparable to AIH in respect of most markers of liver damage and total Ig G. These cases had longer disease duration compared with auto-Ab negative cases, but there was no difference in gender, age or viral load. KLM-1+ Ab CHC cases showed best overlap with AIH. CONCLUSION Auto-Ab levels in CHC may be important markers of disease severity and positive cases have a disease similar to AIH. Auto-Abs might have a pathogenic role as indicated by elevated markers of liver damage. Future studies will unravel any novel associations between these two diseases, whether genetic or other. | Kawa Amin Aram H Rasool Ali Hattem Taha AM Al-Karboly Taher E Taher Jonas Bystrom | 2017 | World Journal of Gastroenterology2017，23，8： | 5 |
| 4 | Lithium difluoro(oxalato)borate as salt for lithium-ion batteries显示文摘 | Chen Z H Liu J Amine K | 2007 | Electrochem Solid-State Lett2007，10，3： | 1 |
| 5 | CT in pseudomyxoma peritonei:a review of cases显示文摘 | Sulkin TV O′Neill H Amin A I | 2002 | Clin Radiol2002，57，7： | 1 |
| 6 | Estimating parameters in continuous univariate distributions with a shifted origin显示文摘 | CHENG R C H AMIN N A K | 1983 | Journal of the Royal Statistical Society Series B (Methodological)1983，45，3： | 1 |
| 7 | Sarcomatoid differentiation in renal cell careinoma显示文摘 | Peralta-Venturina M Moth H Amin M | 2001 | Am J Sury Pathol2001，25，3： | 1 |
| 8 | CT in Pseudomyxoma Peritonei:a Review of 17 Cases显示文摘 | Sulkin TV O'Neill H Amin AI | 2002 | Clin Radiol2002，57，7： | 1 |
| 9 | Inter- leukin-6 and type I interferon-regulated genes and chemokines mark disease activity in dermatomyositis 显示文摘 | BILGIC H YTFERBERG S R AMIN S | 2009 | Arthritis Rheum2009，6，11： | 1 |
| 10 | Grain boundary structure and chemistry of Dy - diffusion processed Nd - Fe - B sintered magnets 显示文摘 | Sepehri - Amin H Ohkubo T Hono K | 2010 | J Appl Phys2010，107，09： | 1 |
| 11 | Polymorphisms of hu- man platelet alloantigens HPA-1 and HPA-2 associated with severe coronary artery disease 显示文摘 | Abboud N Amin H Ghazouani L | 2010 | Cardiovasc Pathol2010，19，5： | 1 |
| 12 | Enhanced anti-tumor activity by the combination of the natural compounds (-)-epigallocatechin-3-gall - ate and luteolin: potential role of p53 显示文摘 | Amin AR Wang D Zhang H | 2010 | J Biol Chem2010，285，34： | 1 |
| 13 | Quantifying the doseresponse of walking I reducing coronary heart disease risk:meta-analysis显示文摘 | Zheng H Orsini N Amin J | 2009 | Eur J Epedemiol2009，24，4： | 1 |
| 14 | An empirical study on the charac- teristics of Gabor representations for face recognition显示文摘 | Amin M A and Yan H | 2009 | International Journal of Pattern Recognition and Artificial Intelligence2009，23，3： | 1 |
| 15 | Development and optimization of an adenovirus production process Amine 显示文摘 | AMINE K OLIVIER H | 2004 | J Gene Med2004，6，： | 1 |
| 16 | Synthesis, chemical characterization, DNA binding, antioxidant, antibacterial, and antifungal activities of ferrocence incorporated selenoureas 显示文摘 | RAJA A H AMIN B MUHAMMAD N T | 2014 | J Biochem Mol Toxico12014，28，2： | 1 |
| 17 | Synthesis and improved electrochemical performance of A1 (OH)a-coated LiO2 cathode materials at elevated temperature 显示文摘 | JANG S B KANG S H AMINE K | 2005 | Electrochimica Acta2005，50，： | 1 |
| 18 | Decreased expression level of SH2 domain-containing protein tyrosine phosphatase- 1 (Shpl) is associated with progression of chronic myeloid leukaemia显示文摘 | Amin HM Hoshino K Yang H | 2007 | J Pathol2007，212，4： | 1 |
| 19 | Enzyme inhibition-based biosensors for food safety and environmental monitoring显示文摘 | Amine A Mohammadi H Bourais I | 2006 | Biosensors and Bioelectronics2006，21，8： | 1 |
| 20 | Prognostic implications of increased QRS duration in patients with moderate and severe left ventricular systolic dysfunction显示文摘 | Silvet H Amin J Padmanabhan S | 2001 | Am J Cardiol2001，88，9： | 1 |
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