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| 1 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 2 | Feature extraction via multi-resolution analysis for short-term load forecasting显示文摘 | Rocha Reis A J Alves da Silva AP | 2005 | IEEE Transactions on Power Systems2005，20，1： | 1 |
| 3 | On thestructural diversity of sialoliths显示文摘 | Alves de Matos AP Carvalho PA Almeida A | 2007 | Microsc Microanal2007，13，5： | 1 |
| 4 | Transenarnel and trans- dentinal penetration of hydrogen peroxide applied to cracked or mi- croabrasioned enamel显示文摘 | Briso AL Lima AP Gon~alves RS | 2014 | Oper Dent2014，39，2： | 1 |
| 5 | The impact of syphilis,HIV 1, anctHIV-2 on pregnancy outcome in Bissau, Guinea- Bis-sau显示文摘 | Labbe AC Mendonca AP Alves AC | 2002 | Sex Transm Dis2002，29，3： | 1 |
| 6 | Structure of the sulfated ct-L-fucan from the egg jelly coat of the sea urchin Strongylocentrotus franciscanus: Patterns of preferential 2-O-and 4-O-sulfation determine sperm cell recognition显示文摘 | Vilela-Silva A-CES Alves AP Valente A-P | 1999 | Glycobiology1999，9，： | 1 |
| 7 | Females of the sea urchin Strongylocentrotus purpuratus differ in the struc- tures of their egg jelly sulfated fucans显示文摘 | Alves AP Mulloy B Moy GW | 1998 | Glycobiology1998，8，： | 1 |
| 8 | Structure of the sulfated alpha-L-fucan from the egg jelly coat of the sea urchin Strongyloeentrotus franciscanus: Patterns of preferential 2-0- and 4-O-sulfation determine sperm cell recognition显示文摘 | Vilela-Silva AC Alves AP Valente AP | 1999 | Glyeobiology1999，9，： | 1 |
| 9 | Carvedilol:relation between antioxidant activity and inhibition of the mitochondrial permeability transition显示文摘 | Oliveira PJ Esteves T Rolo AP Monteiro P Gon?alves L Palmeira CM | 2003 | Rev Port Cardiol2003，22，1： | 1 |
| 10 | High-oleic peanuts:new perspective to attenuate glucose homeostasis disruption and inflammation related obesity显示文摘 | Moreira Alves RD Boroni Moreira AP Macedo VS | 2014 | Obesity(Silver Spring)2014，22，9： | 1 |
| 11 | High -oleic peanuts: new perspective to attenuate glucose homeostasisdisruption and inflammation related obesity 显示文摘 | Moreira Alves RD Boroni Moreira AP Macedo VS | 2014 | Obesity ( SilverSpring)2014，22，9： | 1 |
| 12 | Ptose palpebral associada a paquidermoperiostose:relato de caso显示文摘 | Alves AP Holanda Filha JG Jeronimo FT | 2005 | Arq Bras Oftalmol2005，68，： | 1 |
| 13 | RNA interference as a method for target-site screening in the Western corn rootworm, Dia- brotica virgifera virgifera显示文摘 | Alves AP Lorenzen MD Beeman RW | 2010 | J Insect Sci2010，10，： | 1 |
| 14 | Effect of commereial mouthwashes on the corrosion resistance of Ti- 10Mo experimental alloy显示文摘 | Alves Rezende MC Aires AP Codaro EN | 2007 | J Mater Sci Mater Med2007，18，1： | 1 |
| 15 | Clinicopathological study ofpatients with head and neck sarcomas 显示文摘 | Pacheco IA Alves AP Mota MR | 2011 | Braz J Otorhinolaryngol2011，77，3： | 1 |
| 16 | Antitumor activity of the essential oil from the leaves of croton regelianus and its component ascaridole显示文摘 | Bezerra DP Marinho Filho JD Alves AP | 2009 | Chemistry and Biodiversity2009，6，8： | 1 |
| 17 | The impact of syphilis,HIV - 1, and HIV -2 on pregnancy outcome in Bissau, Guinea - Bissau 显示文摘 | Labbe AC Mendonca AP Alves AC | 2002 | Sex Transm Dis2002，29，3： | 1 |
| 18 | The impact of syphilis,HIV-1,and HIV-2 on pregnancy outcome in Bissau, Guinea-Bissau显示文摘 | Labbe AC Mendonca AP Alves AC | 2002 | SexTransm Dis2002，29，3： | 1 |
| 19 | Odontogenic tu- mors: a 5-year retrospective study in a Brazilian population and analysis of 3406 cases reported in the literature显示文摘 | OSTERNE RL BRITO RG ALVES AP | 2011 | Oral Surg O- ral Med Oral Pathol Oral Radiol Endod2011，111，4： | 1 |
| 20 | The impact of syphilis, HIV-1, and HIV-2 on pregnancy outcome in Bissau, Guinea-Bissau 显示文摘 | Labbe AC Mendonca AP Alves AC | 2002 | Sex Transm Dis2002，29，3： | 1 |
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