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| 1 | Oral hygiene practices,smoking habit,and self-perceived oral malodor among dental students显示文摘 | Almas K Al-Hawish A Al-Khamis W | 2003 | J Contemp Dent Pract2003，4，4： | 2 |
| 2 | Control of free AA levels by phospholipases A2 and lysophospholipid acyltransferases 显示文摘 | GEMA P C ALMA M A DAVID B | 2009 | Biochim Biophys Acta2009，1791，12： | 1 |
| 3 | Copper(II) removal from aqueous solution by organosolv lignin and its recovery显示文摘 | Acemioglu B Samil A Alma M H Gundogan R | 2003 | Appl Polymer Sci2003，89，： | 1 |
| 4 | Severity of Depression, Anxious Distress and the Risk of Cardiovascular Disease in a Swedish Population-Based Cohort显示文摘 | Almas A Forsell Y Iqbal R | 2015 | PLOS ONE2015，10，01： | 1 |
| 5 | TMEM16 proteins produce volume-regulated chloride currents that are reduced in mice lacking TMEM16A显示文摘 | Alma(c)a J Tian Y Aldehni F | | 0，，42： | 1 |
| 6 | On the performance of Ti/SnO2 and Ti/PbO2 anodes in electrochemical degradation of 2-chlorophenol for wastewater treatment显示文摘 | almas S P Renoldi F | 1999 | J Appl Electrochem1999，29，： | 1 |
| 7 | Liquefaction of grapevine cane(Vit is vinisera L)waste and its applicat ion to phenol formaldehyde type adhesive 显示文摘 | Alma M H Basturk M A | 2006 | Indust rial Crops and Products2006，24，2： | 1 |
| 8 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 9 | Damage detection in structures:From mode shape to frequency response function methods显示文摘 | Maia N M M Silva J M M Almas E A M | 2008 | Me-chanical Systems and Signal Processing2008，17，3： | 1 |
| 10 | The effect of additional pleural procedures onto recurrence rates on the spontaneous pneumothorax surgery 显示文摘 | Zeybek A Kalemci S Gurunlu Alma O | 2013 | Iran Red Crescent Med J2013，15，2： | 1 |
| 11 | Dana- age detection in structures:from mode shape to fre- quency response function methods 显示文摘 | Maia N M M Silva J M M Almas E A M | 2003 | Mechanical Systems and Signal Processing2003，17，3： | 1 |
| 12 | Copper (Ⅱ) removal from aqueous solution by organosolv lignin and its recovery 显示文摘 | Acemioglu B Samil A Alma M H | 2003 | Journal of Applied Polymer Science2003，89，6： | 1 |
| 13 | Relationships between Ostrinia nu- bilalis(Lepidoptera: Crambidae) feeding activity, crop technique and mycotoxin contamination of corn kernel in northwestern Italy显示文摘 | Alma A Lessio F Reyneri A | 2005 | Inter- national Journal of Pest Management2005，51，3： | 1 |
| 14 | The genetic characteris- tics of extended-spectrum β-1actamases (ESBLs) among Escherichia coli and Klebsiella pneumoniae isolates from urine specimens显示文摘 | Petrascu M Flonta M Almas A M | 2011 | HVM Bioflux2011，3，2： | 1 |
| 15 | Comparison of FDA-based and PCA-based features in fault diagnosis of automobile gearboxes显示文摘 | GHARAV1AN M H ALMAS G F OHADI A R | 2013 | Neuroeomputing2013，121，： | 1 |
| 16 | Darnge Detection in Structures from Mode Shape to Frequency Response Function Methods 显示文摘 | Maia N M Silva J M Almas E A | 2003 | Mechanical Systems and Signal Processing2003，17，3： | 1 |
| 17 | Speciation of Cd and Zn in contaminated soils assessed by DGT-DIFS,and WHAM/Medel Ⅵ in relation to uptake by spinach and ryegrass显示文摘 | Almas A R Lombnaes P Sogn T A | 2006 | Chemosphere2006，62，10： | 1 |
| 18 | A Proteomics Study of Brassinosteroid Response in Arabidopsis显示文摘 | DENG Z P ZHANG X TANG W Q OSES-PRIETO J A SUZUKI N GENDORN J M CHEN H J GUAN S H CHALKLEY R J PETERMAN T K ALMA L B WANG Z Y | 2007 | Molecular & Cellular Proteomics2007，6，： | 1 |
| 19 | Bio-accumulation and toxicity of lead(Pb) in Lemna gibba L(duckweed)显示文摘 | Alma S Sobrino María G Miranda Carlos Alvarez | | Journal of Environmental Science and Health Part A0，45，： | 1 |
| 20 | Compliance to antihypertensive therapy显示文摘 | Almas A Hameed A Ahmed B | 2006 | J Coll Phys Surg Pak2006，16，1： | 1 |
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