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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents 显示文摘 | Lichtenthaler HK Wellburn AR 1983 | 1983 | Biochemical Society Transaction1983，11，： | 1 |
| 3 | The composing process of advanced ESL students : Six case - studies 显示文摘 | Zamel 1983:165) (Zamel V | 1983 | TESOL Quarterly1983，，17： | 1 |
| 4 | Study on the pollen morphology of thefamily Berberidaceae 显示文摘 | ZHANG KT WANG PL 1983 | 1983 | Acta Phytotax Sin1983，21，2： | 1 |
| 5 | Principles of internal corrosion and corrosion monitoring显示文摘 | Kirmyer G J Logsdon G S 1983 | 1983 | AWWA1983，75，4： | 1 |
| 6 | 14C Tracer evidence for synthesis of choline and betaine via phosphoryl base intermediates in salinized sugarbeet leaves显示文摘 | Hanson AD Rhodes D 1983 | 1983 | Plant Physiology1983，71，： | 1 |
| 7 | Lignans and other constituents from Carissa edulis显示文摘 | Achenbach H Waibel R Ivan AM 1983 | 1983 | Phytochemistry1983，22，3： | 1 |
| 8 | Oak catkins, loves and fruits from the Oligocene Catahoula Formation and their evolutionary significance 显示文摘 | Daghlian CP Crepet WL 1983 | 1983 | Amer J Bot1983，70，： | 1 |
| 9 | 显示文摘 | 李光军 宋蕊.? 咸阳博物馆收藏的两件带铭铜壶(J)考古与文物. 1983 (06) | 1983 | 考古与文物1983，，06： | 1 |
| 10 | The Market for Corporate Control:the Scientific Evidence显示文摘 | MC and RS Ruback 1983 | 1983 | Journal of Financial Economic1983，11，： | 1 |
| 11 | Bioavailability of heavy metals to aquatic organisms-A review显示文摘 | Luoma S N 1983 | 1983 | Science of the Total Environment1983，28，： | 1 |
| 12 | Osseointegratlon and its experi- mental background 显示文摘 | Brnemark PI( September 1983) | 1983 | J Prosthet Dent1983，50，3： | 1 |
| 13 | 显示文摘 | 闻恽 张莲华 徐永利.? 子宫肌瘤的超声图象诊断(J)中华物理医学与康复杂志. 1983 (04) | 1983 | 中华物理医学与康复杂志1983，，： | 1 |
| 14 | Second homes, a framework for policy显示文摘 | Shucksmith D M (1983) | 1983 | Town Planning Review1983，54，： | 1 |
| 15 | Physiological energetics of marine mollusks显示文摘 | Bayne B L Newell R C 1983 | 1983 | The Mollusea1983，4，： | 1 |
| 16 | Simultaneous determination of total nitrogen and phosphorus in water using peroxodisulfate oxidation显示文摘 | Ebina J Tsutsui T Shirai T 1983 | 1983 | Water Research1983，17，： | 1 |
| 17 | Biogeography of oaks in the arcto-tertiary province显示文摘 | Axelord DI 1983 | 1983 | Ann Missouri Bot Gard1983，70，： | 1 |
| 18 | Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters显示文摘 | Box J D 1983 | 1983 | Water Res1983，17，： | 1 |
| 19 | Investigation of the folin-ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters显示文摘 | Box J D 1983 | 1983 | Water Res1983，17，： | 1 |
| 20 | 显示文摘 | 耿温琦 张福东.? 高速涡轮钻在拔下颌阻生智齿时的应用(J)中华口腔医学杂志. 1983 (04) | 1983 | 中华口腔医学杂志1983，，04： | 1 |
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