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| 1 | Isolation and separation of HMG proteins and histones H1 and H5 and core histones by column chromatography on phosphocellulose 显示文摘 | Garg C Reeck G R | 1998 | Protein Expres Purif1998，14，2： | 2 |
| 2 | Cloning, heterologous expression, renaturation, and characterization of a cold - adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp strain B11 - 1 显示文摘 | SUZUKI T NAKAYAMA T | 2003 | Protein Expr Purif2003，30，2： | 1 |
| 3 | Purification early pregnancy factor expressed in Sf9 insect cells and in Escherichia coli 显示文摘 | Somodevilla-Torres M J Morton H and characterisation of functional Zhang B | 2003 | Protein Expr Purif2003，32，2： | 1 |
| 4 | Secre- tion of recombinant pro - and mature fungal - sarein ribo toxin by themethylotmphie yeast P/ch/apastoris : The Lys - arg motif is re- quired for maturation 显示文摘 | MARTINEZ - RUIZ A MARTINEZ A LACADENA J | 1998 | Protein Expr Purif1998，12，3： | 1 |
| 5 | Functional expression,purification,and characterization of the extra stable human placental alkaline phosphatase in the pichia pastoris system显示文摘 | CHEN Y H CHANG T C CHANG G G | 2004 | Protein Expres and Purif2004，36，： | 1 |
| 6 | Expression, purification and kinetic characterization of His-tagged glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi 显示文摘 | Cheleski J Freitas R F Wiggers H J | 2011 | Protein Expr Purif2011，76，2： | 1 |
| 7 | Cloning,ex-pressing,prification and functional characterizationof the oligomerization domain of Bcr-Abl oncopro-tein fused to the cytoplasmic transduction peptide显示文摘 | Huang SF Liu DB Zeng JM | 2009 | Protein Expr Purif2009，64，2： | 1 |
| 8 | Preparation and characterization of the recombinant selenomethionine analogue of insulin-like growth factor-Ⅰ 显示文摘 | De Bree FM Brzozowski AM Gellerfors P | 1998 | Protein Expr Purif1998，13，3： | 1 |
| 9 | High-level intracellular expregsion of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts显示文摘 | Hasslacher M Schall M Hayn M | 1999 | Protein Expr Purif1999，11，： | 1 |
| 10 | Preparation of recombinant murine tumor necrosis factor-alpha in Escherichia coli: a rapid method to remove tags from fusion proteins by thrombincleavage and ion-exchange chromatography 显示文摘 | Tsukamoto H Fukudome K Kohara J | 2007 | Protein Expr Purif2007，56，1： | 1 |
| 11 | Expression and purification of the active va- riant of recombinant nmrine Golli-interacting protein (GIP)-char- acterization of its phosphatase activity and interaction with Golli- BG21 显示文摘 | Bamm VV Harauz G | 2008 | Protein Expr Purif2008，62，1： | 1 |
| 12 | Expression of active recombinant human lactoferrin in the milk of transgenic goats显示文摘 | ZHANG Jie LI Lin CAI Yinfeng | 2008 | Protein Expr Purif2008，57，： | 1 |
| 13 | Partial purification of hu- man parathyroid hormone 1-84 as a thioredoxin fusion form in recombinant Eacherichia coli by thermoosmotic shock 显示文摘 | Guo QR Wei DZ Tong WY | 2006 | Protein Expr Purif2006，49，1： | 1 |
| 14 | Definition, expression, and characterization of a protein domain in the N-terminus of pregnancy-associated plasma protein-A distantly related to the family of laminin G-like modules 显示文摘 | Boldt HB Glerup S Overgaard MT | 2006 | Protein Expr Purif2006，48，2： | 1 |
| 15 | Expression of furin-linked Fab fragments against anthrax toxin in a single mammalian expression vector显示文摘 | Reed CD Rast H Hu WG | 2007 | Protein Expr Purif2007，54，2： | 1 |
| 16 | Production of recombinant bovine lactoferrin N-Lobe in insect cells and its antimicrobial activity显示文摘 | NAKAMURA I WATANABE A TSUNEMITSU H | 2001 | Protein Expres Purif2001，21，3： | 1 |
| 17 | High yield expression and single-step purification of Toxoplasma gondii SAG1,GRA1,and GRA7 antigens in Escherichia coli显示文摘 | Hiszczy(n)ska-Sawicka E Brillowska-Dabrowska A Dabrowski S | 2003 | Protein Expr Purif2003，27，1： | 1 |
| 18 | High-level expression of the recombinant hybrid peptide cecropinA(1-8)-magainin2(1-12) with an ubiquitin fusion partner in Escherichia coli显示文摘 | Xu X Jin F Yu X | 2007 | Protein Expr Purif2007，55，1： | 1 |
| 19 | Expression in Escherichia coli of the thermostable inorganic pyrophosphatase from the Aquifex aeolicus and purieation and characterization of the recombinant enzyme 显示文摘 | Hoe HS Kim HK Kwon ST | 2001 | Protein Expr Purif2001，23，2： | 1 |
| 20 | An unusual halo tolerant alpha-type carbonic anhydrate from the alga Unadilla Salina functionally expressed in Escherichia coli显示文摘 | Premkumar L Bageshwar UK Gokhman I | 2003 | Protein Expr Purif2003，28，1： | 1 |
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