Use of fluorescent europium chelates as labels for detection of microcystin-LR in Taihu Lake, China

查看全文 作　　者：[1]钮伟民;[1]何恩奇;[1]吴庆刚;[1]周伟杰;[2]张艺;[2]黄飚;[3]赵晓联

机构地区：[1]Laboratory Department of Municipal Center for Disease Control and Prevention;[2]Key Laboratory of Nuclear Medicine,Department of Biotechnology,Jiangsu Institute of Nuclear Medicine;[3]Jiangsu Suwei Microbiology Research Co.,Ltd.

出　　处：《Journal of Rare Earths》索引2012年第30卷第9期,共6页

基　　金：Project supported by Foundation of Public Health Department of Jiangsu (HD200865);National High-tech Research and Development Program (863 program) (2008AA10Z415) 摘　　要：A method using the time-resolved fluorescence technology to establish a highly sensitive microcystin-LR (MC-LR) indirect competitive immunoassay was proposed in this work. This method was used to monitor the MC-LR level in source water and treated drinking water from Taihu Lake. Algae in the water samples were removed by centrifugation, and the MC-LR level was quantified using this method. Testing results showed that the sensitivity of this method was 0.01 μg/L, and the dynamic measuring range was from 0.05 to 2 μg/L. The average recovery was 115%, and the variation (CV) within and between different batches were 7.3% and 9.7%, respectively. Testing results also indicated that this time-resolved fluoroimmunoassay was sensitive and accurate in measuring MC-LR level, especially for quantitative analysis MC-LR level in bulk water. 关键词：微囊藻毒素-LR 荧光技术标签检测太湖螯合物中国时间分辨测量范围

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Use of fluorescent europium chelates as labels for detection of microcystin-LR in Taihu Lake, China

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