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Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method

查看全文 作　　者：SUN [1,2]Yue;HE [1,2]LiPing;DAI [2]Jun;WANG [2]JingYi;LÜ [2]HuiBin;WANG [1]ShuFang;JIN [2]KuiJuan;ZHOU [2]YueLiang;YANG [2]GuoZhen 高影响力作者 机构地区：[1]College of Physics Science and Technology,Hebei University,Baoding 071002,China;[2]Beijing National Laboratory,for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190,China高影响力机构 出　　处：《Chinese Science Bulletin》索引2012年第57卷第22期,共3页高影响力期刊 基　　金：supported by the National Basic Research Program of China(2007CB935701) 摘　　要：The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method.We achieved the two-dimensional scanning images of OIRD Re{△ p △s } intensities before and after interactions as well as the curves of reaction dynamic processes.The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays,but also can real-time monitor the kinetic processes of biomolecular interactions. 关 键 词：实时检测 无标记 反射率 斜入射 特异性结合 生物分子相互作用 G蛋白 免疫球蛋白G
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