A bioaugmentation failure caused by phage infection and weak biofilm formation ability

查看全文 作　　者：FU [1,2]Songzhe;FAN [1,2]Hongxia;LIU [1]Shuangjiang;LIU [3]Ying;LIU [1]Zhipei

机构地区：[1]State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;[2]Graduate University of Chinese Academy of Sciences, Beijing 100039, China;[3]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266701, China

出　　处：《Journal of Environmental Sciences》索引2009年第21卷第8期,共9页

基　　金：supported by the Hi-Tech Research and Development Program (863) of China (No.2006AA100305) 摘　　要：Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAF. Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, grazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9–18. 关键词：噬菌体感染生物强化生物膜形成能力异养硝化细菌曝气生物滤池氨氮去除率荧光原位杂交

相关文献

参考文献（38）
引证文献（5）
耦合文献（0）

参考文献(38)

引证文献(5)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

A bioaugmentation failure caused by phage infection and weak biofilm formation ability

参考文献(38)

引证文献(5)

用户提示