Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis

查看全文 作　　者：Di Na Fu-Nan Liu Zhi-Feng Miao Zong-Min Du Hui-Mian [1]Xu

机构地区：[1]Department of Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第5期,共8页

基　　金：Supported by The National Natural Science Foundation of China, NSFC, 30672050 摘　　要：AIM: To determine the inhibitory effect of Astragalus memebranaceushas on gastric cancer cell supernatantinduced apoptosis of human peritoneal mesothelial cells. METHODS: Human peritoneal mesothelial cell (HPMC) line HMrSV5 was co-incubated with gastric cancer cell supernatant (MKN45) and/or Astragalus memebranaceushas. Morphological changes in gastric cancer cells were observed under phase-contrast microscope. Quantitative cell damage was determined by MTT assay. Apoptosis was determined under transmission electron microscope and quantified by detecting acridine orange/ethidium bromide-stained (AO/EB) condensed nuclei under fluorescent microscope or by flow cytometry. Expressions of Bcl-2 and Bax were evaluated with immunostaining. RESULTS: Morphological changes and exfoliation occurred and naked areas appeared in cultured HMrSV5 cells 24 h after they were treated with gastric cancer cell supernatant. Cell supernatant from MKN45 gastric cancer cells induced apoptosis of HMrSV5 cells in a time-dependent manner. Obvious morphological changes were observed in cell apoptosis, such as condensation of chromatin, nuclear fragmentations and apoptotic bodies. Astragalus memebranaceus could partly suppress these changes and regulate the expressions of Bcl-2 and Bax in HMrSV5 cells.CONCLUSION: Gastric cancer cells induce apoptosis of HPMCs through the supernatant. Astragalus memebranaceushas inhibits this phenomenon and can be used an adjuvant chemothera-peutic agent in gastric cancer therapy. 关键词：胃癌间皮细胞癌细胞化疗诊断

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Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis

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