Identification of the major allergen of Macrobrachium rosenbergii (giant freshwater prawn)

查看全文 作　　者：Zailatul Hani Mohamad [1]Yadzir;Rosmilah [2]Misnan;Noormalin [1]Abdullah;Faizal [1]Bakhtiar;Masita [1]Arip;Shahnaz [1]Murad

机构地区：[1]Allergy and Immunology Research Centre,Institute for Medical Research,Jalan Pahang;[2]Department of Biology,Faculty of Science and Mathematics,Universiti Pendidikan Sultan Idris

出　　处：《Asian Pacific Journal of Tropical Biomedicine》索引2012年第2卷第1期,共5页

基　　金：Supported by a research grant from UPSI(grant No.UPSI2011-0018-102-01) 摘　　要：Objective:To characterize the major allergens of Macrobrachium rosenbergii.(giant freshwater prawn).Methods:Raw and cooked extracts of the giant freshwater prawn were prepared.The IgE reactivity pattern was identified by sodium dodecyl sulfate polyaerylamide gel electrophoresis(SDS-PAGE)and immunoblotting technique with the sera of 20 skin prick test(SPT)positive patients.The major allergen identified was then characterized using the proteomics approach involving a combination of two-dimensional(2-DE)electrophoresis,mass spectrometry and bioinformatics tools.Results:SDS-PAGE of the raw extract showed 23 protein bands(15-250 kDa)but those ranging from 40 to 100 kDa were not found in the cooked extract.From immunoblotting experiments,raw and cooked extracts demonstrated 11 and 5 IgE-binding proteins,respectively,with a molecular mass ranging from 15 to 155 kDa.A heat-resistant 36 kDa protein was identified as the major allergen of both extracts.In addition,a 42 kDa heat-sensitive protein was shown to be a major allergen of the raw extract.The 2-DE gel fractionated the prawn proteins to more than 50 different protein spots.Of these,10 spots showed specific:IgE reactivity with patients'sera.Matrix assisted laser desorption/ionization-lime of flight(MALDI-TOF)analysis led to identification of 2 important allergens,tropomyosin and arginine kinase.Conclusions:It can be concluded that the availability of such allergens would help in component-based diagnosis and therapy of prawn allergies. 关键词：MACROBRACHIUM rosenbergii Major ALLERGEN MALDI-TOF TROPOMYOSIN ARGININE kinase SDS-PAGE Immunoblotting 2-DE electrophoresis IgE reactivity

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Identification of the major allergen of Macrobrachium rosenbergii (giant freshwater prawn)

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