维普中文期刊产品整合服务

Distribution of T-DNA carrying a Ds element on rice chromosomes

查看全文 作　　者：WANG Jiang LI Lin WAN Xinshan AN Linsheng ZHANG [1]Jingliu 高影响力作者 机构地区：[1]National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China高影响力机构 出　　处：《Science China(Life Sciences)》索引2004年第47卷第4期,共10页高影响力期刊 摘　　要：Over 3000 rice plants with T-DNA carrying a Ds element were constructed by Agro-bacterium tumefaciens mediation. Using inverse PCR methodology, 590 unique right flanking sequences of T-DNA (Ds) were retrieved from independent transformants and classified into six main types on the basis of the origin of filler DNA between the right border of T-DNA and flanking sequence of rice genome. Type I sequences were the most common and showed canonical in-tegration that T-DNA right border was followed by rice genome sequence with or without filler DNA of no more than 50 bp, while type II sequences displayed a vector-genome combination that T-DNA right border was followed by a vector fragment and then connected with rice genome sequence. The location and distribution of 340 type I and II flanking sequences on the rice chromosome were determined using BLAST analysis. The 340 Ds insertions at an average in-terval of 0.8 megabase (Mb) constructed a basic framework of Ds starter points on whole rice chromosomes. The frequency of T-DNA (Ds) inserted into the exons of predicted genes on chromosome one was 21%. Knowledge of T-DNA (Ds) locations on chromosomes will prove to be a useful resource for isolating rice genes by Ds transposon tagging as these Ds insertions can be used as starting lines for further mutagenesis. 关 键 词：DS element,T-DNA,mutagenesis,rice(Oryza SATIVA L.).
相关文献

参考文献(16)

引证文献(5)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费