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| 1 | Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis显示文摘The whitefly Bemisia tabaci are considered as a taxonomically complex that contained some destructive pests.Two of the most prevalent cryptic species are B.tabaci Middle East-Asia Minor 1(MEAM1)and Mediterranean(MED).In an extensive field survey of the B.tabaci complex present throughout part of China from 2004 to 2007,we obtained 93 samples of B.tabaci from 22 provinces.We determined that these Chinese haplotypes included 2 invasive species(MEAM1 and MED),and 4 indigenous cryptic species(Asia II 1,Asia II 3,China 3 and Asia II 7)by sequencing mitochondrial cytochrome oxidose one gene(mtCOI).The diversity and genetic differentiation of a subset of 19 populations of B.tabaci were studied using cDNA amplified fragment length polymorphism(AFLP).Prior to 2007,MEAM1 was a dominant species in many provinces in China.By 2007,MED was dominant in 11 provinces.Both invasive and indigenous species were simultaneously found in some regions.Indigenous species of B.tabaci were found in six provinces in southern China.MED and MEAM1 have broad ranges of host plants,and indigenous species appeared to have much narrower host ranges.All Asia II 3 samples were found on cotton except one on aubergine.China 3 has more host plants than Asia II 3.Twelve samples of China 3 were collected from sweet potato,Japanese hop,squash and cotton.A total of 677 reproducible bands amplified with 5 AFLP primer combinations were obtained.The highest proportion of polymorphic bands was 98.7% and the lowest was 91.9%.Unweighted pair-group method analysis indicated that the clustering was independent of the different species.MED showed the lowest degree of similarity than the other species.The data indicate that both MEAM1and MED were rapidly established in China. | GUO Xiao-jun RAO Qiong ZHANG Fan LUO Chen ZHANG Hong-yu GAO Xi-wu | 2012 | Journal of Integrative Agriculture2012，11，2： | 14 |
| 2 | Genome-wide identification and characterization of HSP gene superfamily in whitefly (Bemisia tabacl)and expression profiling analysis under temperature stress显示文摘Heat shock proteins (HSP)are essential molecular chaperones that play important roles in the stress stimulation of insects.Bemisia tabaci,a phloem feeder and invasive species,can cause extensive crop damage through direct feeding and transmission of plant viruses.Here we employed comprehensive genomics approaches to identity HSP superfamily members in the Middle East Asia Minor 1 whitefly genome.In total,we identified 26 Hsp genes,including three Hsp90,17 Hsp70,one Hsp60 and five sHSP (small heat shock protein)genes.The HSP gene superfamily of whitefly is expanded compared with the other five insects surveyed here.The gene structures among the same families are relatively conserved.Meanwhile,the motif compositions and secondary structures of BtHsp proteins were predicted.In addition,quantitative polymerase chain reaction analysis showed that the expression patterns of BtHsp gene superfamily were diverse across different tissues of whiteflies.Most Hsp genes were induced or repressed by thermal stress (40℃)and cold treatment (4℃)in whitefly.Silencing the expression of BtHsp70-6 significantly decreased the survival rate of whitefly under 45℃.All the results showed the Hsps conferred thermo-tolerance or cold-tolerance to whiteflies that protect them from being affected by detrimental temperature conditions.Our observations highlighted the molecular evolutionary properties and the response mechanism to temperature assaults of Hsp genes in whitefly. | Xin-Ru Wang Chao Wang Fei-Xue Ban Dan-Tong Zhu Shu-Sheng Liu Xiao-Wei Wang | 2019 | Insect Science2019，26，1： | 11 |
| 3 | Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population显示文摘Bemisia tabaci adults from various host-plant species were collected from 31 regions across India.266 B.tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints.Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one(mtCOI)genes.Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had <3.5% sequence divergence from groups currently termed Asia I,Asia II-5,Asia II-7,and Asia II-8.The biotype-B B.tabaci from India clustered into the Middle East-Asia Minor 1 group.A new group of B.tabaci from Coimbatore,collected from pumpkin,was related most closely to the Asia I group(6.2% sequence divergence from the consensus Asia I sequence).To increase our understanding of the epidemiology of tomato leaf curl disease(ToLCD)and the key B.tabaci genetic groups involved in virus spread,the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus(ToLCBV).The acquisition access periods(AAP),inoculation access periods(IAP),latent periods(LP),and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics.These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India. | R V Chowda-Reddy M Kirankumar Susan E Seal V Muniyappa Girish B Valand M R Govindappa John Colvin | 2012 | Journal of Integrative Agriculture2012，11，2： | 8 |
| 4 | Next Generation Transcriptome Sequencing and Quantitative Real-Time PCR Technologies for Characterisation of the Bemisia tabaci Asia 1 mtCOI Phylogenetic Clade显示文摘A programme of functional genomics research is underway at the University of Greenwich,UK,to develop and apply genomics technologies to characterise an economically-important but under-researched Bemisia tabaci(Hemiptera:Aleyrodidae),the Asia 1 mtCOI phylogenetic group.A comparison of this putative species from India with other important B.tabaci populations and insect species may provide targets for the development of more effective whitefly control strategies.As a first step,next-generation sequencing(NGS)has been used to survey the transcriptome of adult female whitefly,with high quality RNA preparations being used to generate cDNA libraries for NGS using the Roche 454 Titanium DNA sequencing platform.Contig assemblies constructed from the resultant sequences(301 094 reads)using the software program CLC Genomics Workbench generated 3 821 core contigs.Comparison of a selection of these contigs with related sequences from other B.tabaci genetic groups has revealed good alignment for some genes(e.g.,HSP90)but misassemblies in other datasets(e.g.,the vitellogenin gene family),highlighting the need for manual curation as well as collaborative international efforts to obtain accurate assemblies from the existing next generation sequence datasets.Nevertheless,data emerging from the NGS has facilitated the development of accurate and reliable methods for analysing gene expression based on quantitative real-time RT-PCR,illustrating the power of this approach to enable rapid expression analyses in an organism for which a complete genome sequence is currently lacking. | Susan Seal Mitulkumar V Patel Carl Collins John Colvin David Bailey | 2012 | Journal of Integrative Agriculture2012，11，2： | 2 |
| 5 | Effects of cucumber mosaic virus-infected chilli plants on non-vector Bemisia tabaci (Hemiptera Aleyrodidae)显示文摘Plant virus infections are known to alter host plant attractiveness and suitability for insect herbivores.This study was conducted to determine how cucumber mosaic virus (CMV)-infected chilli plants affect the fitness and settling preferences ofnonvector whitefly,Bemisia tabaci adults under dual-choice conditions with volatile organic compounds analyzed using solid phase microextraction coupled with gas chromatography-mass spectrometry (GC-MS).Results showed that the presence of CIVIV in chilli plants substantially affects the settling preferences of the B.tabaci,which preferred to settle on noninfected plants.Duration of the egg stage and the longevity and fecundity of adult B.tabaci on CMV-infected chilli plants were not markedly different from those on noninfected chilli plants.In contrast,the developmental time from egg to adult was significantly reduced in CMV-infected chilli plants compared to the noninfected plants.The results also showed that CMV-infected chilli plants released significantly more linalool and phenylacetaldehyde than noninfected plants.Overall,it was suggested that the behavioral response of B.tabaci might be modified by CMV-infected plants,which alter the release of specific headspace volatiles.Based on these results,the modification of plant volatile profiles may help in enhancing the effectiveness of biological control and the protection of crop plants against B.tabaci. | Khalid A.Saad M.N.Mohamad Roff Rebecca H.Hallett Idris B.Abd-Ghani | 2019 | Insect Science2019，26，1： | 1 |
| 6 | Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci显示文摘Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent,circulative manner by homopteran insects.Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of F2 genotypes of Schizaphis graminum segregating for virus transmission ability,we recently discovered a panel of protein biomarkers that predict vector competency.Here we used aphid and whitefly nucleotide and expressed sequence tag database mining to test whether these biomarkers are conserved in other homopteran insects.S.graminum gene homologs that shared a high degree of predicted amino acid identity were discovered in two other aphid species and in the whitefly Bemisia tabaci.Selected reaction monitoring mass spectrometry was used to validate the expression of these biomarkers proteins in multiple aphid vector species.The conservation of these proteins in multiple insect taxa that transmit plant viruses along the circulative transmission pathway creates the opportunity to use these biomarkers to rapidly identify insect populations that are the most efficient vectors and allow them to be targeted for control prior to the spread of virus within a crop. | Michelle Cilia Michael Bereman Tara Fish Michael J MacCoss stewart Gray | 2012 | Journal of Integrative Agriculture2012，11，2： | 1 |
| 7 | Patterns of host cell inheritance in the bacterial symbiosis of whiteflies显示文摘Whiteflies possess bacterial symbionts Candidatus Portiera aleyrodidium that are housed in specialized cells called bacteriocytes and are faithfully transmitted via the ovary to insect offspring.In one whitefly species studied previously,Bemisia tabaci MEAMI,transmission is mediated by somatic inheritance of bacteriocytes,with a single bacteriocyte transferred to each oocyte and persisting through embryogenesis to the next generation.Here,we investigate the mode of bacteriocyte transmission in two whiefly species,B.tabaci MED,the sister species of MEAM1,and the phylogenetically distant species Trialeurodes vaporariorum.Microsatllite analysis supported by microscopical studies demonstrates that B.tabaci MED bacteriocytes are genetically different from other somatic cells and persist through embryogenesis,as for MEAMI,but T.vaporariorum bacteriocytes are genetically identical to other somatic cells of the insect,likely mediated by the degradation of maternal bacteriocytes in the embryo.These two alternative modes of transmission provide a first demonstration among insect symbioses that the cellular processes underlying vertical transmission of bacterial symbionts can diversify among related host species associated with a single lineage of symbiotic bacteria. | Xiao-Rui Xu Na-Na Li Xi-Yu Bao Angela E.Douglas Jun-Bo Luan | 2020 | Insect Science2020，27，5： | 0 |
| 8 | Impact of a novel Rickettsia symbiont on the life history and virus transmission capacity of its host whitefly(Bemisia tabaci)显示文摘Rickettsia consists of some of the most prevalent symbionts of insects and often plays a significant role in the biology of its hosts.Recently,a maternally inherited Torix group Rickettsia,provisionally named as RiTBt,was recorded in a species of notorious pest whitefly,tentatively named as Asia II 1,from the Bemisia tabaci complex.The role of this Rickettsia in the biology of its host is unknown.Here we investigated the impact of RiTBt on the performance and virus transmission capacity of Asia II 1.RiTBt did not significantly affect the life history parameters of the whitefly when the host insect was reared on tobacco,tomato,and cotton,three host plants with relatively low,medium and high suitability to the whitefly.Intriguingly,RiTBt slightly enhanced whitefly transmission of cotton leaf curl Multan virus(CLCuMuV),a virus that is transmitted by the whitefly in the field and has caused extensive damage to cotton production.Specifically,compared with whiteflies without RiTBt,following a 48 h virus acquisition whiteflies with RiTBt had higher titer of virus and showed higher efficiency of virus transmission.A rickettsial secretory protein BtR242 was identified as a putative virus-binding protein,and was observed to interact with the coat protein of CLCuMuV in vitro.Viral infection of the whitefly downregulated gene transcript levels of the BtR242 gene.These observations indicate that RiTBt has limited impact on the biology of the Asia II 1 whitefly,and whether this symbiont has functions in the biology of other host whiteflies warrants future investigation. | Teng Lei Jing Zhao Hua-Ling Wang Yin-Quan Liu Shu-Sheng Liu | 2021 | Insect Science2021，28，2： | 0 |
| 9 | Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts显示文摘Nutritional mutualism between insects and symbiotic bacteria is widespread.The various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple secondary symbionts.It is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect hosts.Although effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well understood.We examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the B.tabaci whitefly complex,provisionally named as Asia II 3 and China 1.Genomic sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B vitamins.We then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously reported.The analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were identical.Our findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host whiteflies.Although phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B vitamins.Such metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution. | Dan-Tong Zhu Qiong Rao Chi Zou Fei-Xue Ban Juan-Juan Zhao Shu-Sheng Liu | 2022 | Insect Science2022，29，2： | 0 |
| 10 | Prediction of Whitefly Population and Infestation Based on Multitrophic Analysis between Competitor Pest,Plant Traits and Natural Enemy显示文摘Interaction among arthropods(insect-acarine)was investigated at all trophic levels in agro-ecosystem and affects the population dynamic and diversification of arthropod pests and intensity of natural enemy(parasitism)as well as stimulates the plant chemical defence.In the present study of two cropping periods,nutrient concentration and early infestation of plant-sucking pests are known to trigger different degrees of interactions(plant’s parameters)which potentially alert abundance and diversity of the insect pests.Clearer interaction and magnitude of impact could be assessed(multivariate analysis of variance(MANOVA)and redundancy analysis(RDA))and observed between arthropods(insect-acarine)community and plants’parameters from the strongest to the lowest effects.The two factors could have affected the community of insect-pests with various degrees of pressure effect and interaction that occurred naturally,thus leading to the predictable abundance of insect-acarine populations under eggplant leaves in response to plant physical characteristics(e.g.,size of leaves,number of leaves and plant height)and biochemical constituents(flavonoid,phenolic,peroxidase andβ-1,3-glucanase).Based on multivariate analyses of multitrophic interaction,MANOVA and RDA have the potential to elucidate the complex interaction among plant performance,abundance of pests and activity chemical defence compounds.Both analyses interpreted similar interactions of measured parameters in different ways.Whitefly population in this study was predictable by aphids,thrips and total phenolic contents in eggplant.Eggplant has capable defence systems against insect-acarine pests after stimulation(pre-infestation).In relation to IPM strategy,early stages of plant growth are known as susceptible periods for pests attack but the plant becomes more tolerant during the fruiting stage. | Mohd Rasdi Zaini Noor Shuhaina Shaikh Mazran Che Salmah Md Rawi Alshami Salman | 2019 | Journal of Agricultural Science and Technology(A)2019，9，5： | 0 |
| 11 | Catalase promotes whitefly adaptation to high temperature by eliminating reactive oxygen species显示文摘Thermal stress usually leads to excessive production of reactive oxygen species(ROS)in all aerobic organisms.Catalases(CAT)are the key antioxidant enzymes,which act as the first line of defense against ROS in the antioxidant pathway.The highly invasive and widely distributed whitefly Bemisia tabaci MED damages plants by feeding as well as by transmitting many plant viruses.Previous studies have shown that strong adaptability to high temperature helps explain the spread of MED around the world.However,the mechanism underlying high temperature adaptation of this pest is not well understood.In this study,6 CAT genes were identified from the MED genome and transcriptome dataset,among which BtCAT1,BtCAT2,and BtCAT3 were found to be highly expressed in adults.The expression of BtCAT1,BtCAT2,or BtCAT3 increased with induction temperature and induction time.The MED was exposed with mean high temperature(30℃ or 35℃)and a short-term extremely high temperature(39℃ or 41℃)after the silencing of BtCAT1,BtCAT2,or BtCAT3 to significantly increased ROS levels by at least 0.5 times and significantly decreased survival rate and fecundity of MED adults.The ROS level in the treated specimens gradually returned to a normal level after 24 h at 25℃,but the survival rate still declined significantly.Taken together,our results demonstrate that CAT could help B.tabaci adapt to long-term mean high temperatures and short-term extremely high temperatures by eliminating excessive ROS. | Peng Liang Jie Ning Wenlu Wang Pu Zhu Lianyou Gui Wen Xie Youjun Zhang | 2023 | Insect Science2023，30，5： | 0 |
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