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| 1 | 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用显示文摘JIP-测定(JIP-test)是以生物膜能量流动为基础建立的分析方法。利用该方法可以获得有关光系统II的大量信息。文章介绍了快速叶绿素荧光诱导动力学曲线的定义、数据分析方法及相关参数的意义,并举例说明如何利用该方法分析不同环境条件对光合机构主要是PSII的供体侧、受体侧及PSII反应中心的影响。 | 李鹏民 高辉远 Reto J.Strasser | 2005 | 植物生理与分子生物学学报2005，31，6： | 568 |
| 2 | 铅胁迫对玉米幼苗叶片光系统功能及光合作用的影响显示文摘通过同时测定玉米叶片的叶绿素荧光快速诱导动力学曲线和对820nm光的吸收、分析叶片的气体交换过程以及叶绿体活性氧清除关键酶的活性,研究了不同浓度的铅(Pb)胁迫对玉米光系统Ⅰ(PSⅠ)、光系统Ⅱ(PSⅡ)的光化学活性和光合作用的影响,并分析了Pb胁迫下两个光系统的相互关系。结果表明:铅胁迫显著抑制了玉米地上部分和地下部分的生长、降低了叶片光合色素含量、并通过非气孔因素限制了光合作用、导致过剩激发能的增加;铅胁迫显著抑制了超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)的活性、伤害了PSII反应中心、PSII的受体侧和供体侧(放氧复合体)以及PSI光化学活性。 | 姚广 高辉远 王未未 张立涛 部建雯 | 2009 | 生态学报2009，29，3： | 68 |
| 3 | Regulation of Photosynthesis during Abiotic Stress-Induced Photoinhibition显示文摘无柄的有机体连续地暴露于强加众多的有害效果并且引起产量的巨大的损失的不能生活的压力条件的植物。包括高日光,不能生活的压力在植物的光合的机械上授与严重损坏。Photosystem II (PSII ) 是忍受不能生活的应力的中心力量的光合的机械的最易受影响的部件之一。除了由不能生活的应力的反应的氧种类(ROS ) 的产生, ROS 能也由轻收获的建筑群源于过多的日光的吸收。ROS 能损坏光合的仪器,特别地 PSII,在表明小径和 PSII 修理的抑制的光合的氧化还原作用由于不平衡导致 photoinhibition。与忍耐将要求 ROS 发信号和各种各样的部件的规章的功能的全面理解的改进不能生活的应力设计植物,包括蛋白质 kinases,抄写因素,和植物激素,在到不能生活的应力的光合的机械的回答。Bioenergetics 来临,例如叶绿素短暂动力学分析,在不利环境条件下面便于我们植物活力的理解和对 PSII 效率的评价。这评论讨论当前的理解并且在不能生活的应力下面在光合的机械的规定上显示进一步的研究的潜在的区域。 | Mayank Anand Gururani Jelli Venkatesh Lam-Son Phan Tran | 2015 | Molecular Plant2015，8，9： | 39 |
| 4 | 回忆光合作用研究五十年显示文摘值此沈允钢老师九十大寿即将来临之际,笔者回顾自己50多年的光合作用研究,往事历历在目,恍若昨天。饮水思源,师恩难忘。草成此文,谨表贺忱。1光合强度的田间电测法1960年,笔者以农业科学为第一志愿考入沈阳农学院(现名沈阳农业大学)当年新设置的植物生理生化专业(学制5年)。尽管是不少人羡慕的"尖端专业",可是由于不是心仪已久的果树专业,起初自己并不像其他同学那么喜爱。 | 许大全 | 2016 | 植物生理学报2016，52，11： | 17 |
| 5 | 外施褪黑素对盐胁迫下番茄幼苗光合功能的影响显示文摘褪黑素是植物响应多种非生物胁迫的一种重要的保护因子。为了明确褪黑素对盐胁迫下番茄叶片光合机构功能的影响,以番茄品种‘辽园多丽’为实验材料,利用150mmol·L^(-1)NaCl水培方式做盐处理,并设150μmol·L^(-1)的褪黑素预处理组,研究外施褪黑素对盐胁迫下番茄叶片PSII的影响。结果表明:盐胁迫导致番茄叶片叶绿素含量降低、光合作用减弱,PSII活性的降低主要表现为PSII电子供体侧OEC的活性降低及PSII电子受体侧电子由QA向QB的传递过程受阻,特别是PSII电子供体侧对盐胁迫的敏感程度明显大于受体侧,PSII中D1和Psb O蛋白均发生降解,表明番茄叶片PSII反应供体侧和受体侧受到严重损伤; PSII反应中心对光能的捕获及利用能力降低,单位面积有活性反应中心的数量和吸收的光能用于电子传递的能量比例均有不同程度的下降;外施褪黑素使叶绿素含量和光合速率的降低幅度明显减小,褪黑素通过平衡PSII的供体侧、受体侧和反应中心的电子传递来缓解盐胁迫对番茄PSII造成的伤害,提高番茄的耐盐性。 | 尹赜鹏 王珍琪 齐明芳 孟思达 李天来 | 2019 | 生态学杂志2019，38，2： | 17 |
| 6 | 条锈病对小麦(Triticum aestivum L.)叶片光合功能及光合功能蛋白D1表达的影响显示文摘测定了小麦(Triticum aestivum L.)感染小麦条锈病后的光合常数,以及叶绿素含量、类囊体膜光合电子传递速率和光合反应中心D1蛋白的变化。实验显示,条锈病侵染导致感病小麦叶片净光合速率与叶绿素含量降低;抗病小麦经侵染后净光合速率却有恢复过程,叶绿素含量先降后升。此外,感病小麦叶片被侵染后全链电子传递速率受到抑制,PSII电子传递速率的变化与全链电子传递速率的变化趋势相似,但PSI电子传递速率受到的影响较小;抗病小麦小麦叶片被侵染后电子传递速率所受影响较小。同时发现,病程中,感病和抗病小麦PSII的光合反应中心D1蛋白含量变化总是与PSII电子传递速率的变化类似,推测D1蛋白的表达量变化是引起PSII电子传递活性与全链电子传递速率变化的主要因素之一。 | 沈喜 李红玉 贾秋珍 冯汉青 李敏权 粱厚果 | 2008 | 生态学报2008，28，2： | 16 |
| 7 | NaCl胁迫对菠菜PSII颗粒荧光特性的影响显示文摘使用100、150和200mmol/L浓度的NaCl盐溶液处理菠菜PSII颗粒,观察PSII颗粒在不同浓度、不同时间盐分胁迫下的荧光变化。100mmol/LNaCl即对PSII颗粒荧光造成显著影响,随着NaCl盐溶液浓度的增加,PSII颗粒的室温荧光激发光谱谱峰有明显的下降;不同时间处理下,PSII颗粒的室温荧光激发光谱峰值随胁迫时间延长,也有明显的降低;不同浓度、不同时间盐分胁迫下,PSII颗粒的电子传递速率也受到明显抑制,比对照有显著下降。结果表明,盐分胁迫对菠菜PSII颗粒光合活性造成显著抑制。 | 王弋博 王建平 李勃 杨亚军 安黎哲 | 2005 | 草业学报2005，14，1： | 13 |
| 8 | 叶绿体PSⅡ光能耗散机制的研究进展显示文摘植物的光合作用包括光能固定和过剩光能的耗散两个方面,在光能耗散方面,除了人们以前所认识的光呼吸、Mehler反应等生化机制外,近几年人们发现在光合系统Ⅱ(PSⅡ)复合体内还存在三种光能耗散方式:a.围绕PSⅡ的电子循环;b.与类囊体膜的能量化和叶黄素循环有关的热耗散;c与PSⅡ反应中心异质化及D1蛋白修复循环有关的能量耗散机制. | 李晓萍 陈贻竹 郭俊彦 | 1996 | 生物化学与生物物理进展1996，23，2： | 13 |
| 9 | 叶绿素延迟荧光的发生及其在光合作用研究中的应用显示文摘叶绿素延迟荧光主要由绿色植物中光系统II的天线色素产生,光系统II反应中心色素P680接受天线色素吸收的光能后转变为激发态的P680*,P680*回到基态时释放出一个电子传给原初电子受体,随后电子沿光合电子传递链向PSI传递。当进入电子传递链的电子发生电荷重组时会使P680再次激发形成P680*,P680*将激发能传递给天线色素后,激发能以荧光的形式释放出来,即为延迟荧光。延迟荧光的检测和分析技术为无损测定植物光合机构的结构与功能变化提供了新的方法。利用该方法可以获得丰富的光合机构信息,如光系统II受体侧及供体侧的伤害程度、跨类囊体膜质子梯度的大小等。本文介绍了延迟荧光的产生原理和测定方法,并且举例说明了延迟荧光测定技术在光合作用研究中的应用。 | 杨程 李鹏民 张子山 Vasilij Goltsev 高辉远 | 2013 | 植物生理学报2013，49，12： | 12 |
| 10 | 高温胁迫下外源一氧化氮对生姜叶片多胺代谢及PSII的调控作用显示文摘【目的】生姜具喜温怕热的特性,生产中极易遭受高温伤害。文章旨在通过研究高温胁迫条件下外源NO与多胺代谢的关系,以及对PSII的调控作用,探讨外源NO缓解生姜高温胁迫的生理机制。【方法】以砂培‘莱芜大姜’为试材,置光周期12 h/12 h、昼夜温度28℃/18℃和38℃/28℃的光照培养箱内,分别用0.1 mmol·L-1硝普钠(SNP,NO供体)和铁氰化钠(SF,SNP释放NO后的产物)处理植株根系,分别于处理后0、5、10、15和20 d测定功能叶片的相对含水量、叶绿素含量、电解质渗透率、叶绿素荧光参数及多胺代谢途径相关指标。【结果】高温胁迫导致生姜叶片电解质渗透率增加,相对含水量及叶绿素含量降低;随着高温胁迫时间的延长,叶片最大光化学效率(Fv/Fm)、实际光化学效率(ФPSII)、天线转化效率(Fv′/Fm′)、光化学猝灭系数(qP)和P持续降低,非光化学猝灭(NPQ)、β/α-1和D显著升高;主要表现为PSII反应中心的光化学活性降低,即能量代谢途径由光化学反应转为非光化学反应。高温胁迫下游离态、结合态多胺含量先升后降,束缚态多胺持续上升,腐胺/多胺(Put/PAs)比值显著升高。外源添加NO可恢复叶片相对含水量和叶绿素含量,降低电解质渗透率,促使叶绿素荧光参数趋于正常水平,提高高温胁迫下多胺代谢水平,恢复Put/PAs比值。【结论】38℃/28℃的高温胁迫导致生姜叶片受损,PSII功能紊乱,多胺代谢异常;外源添加0.1 mmol·L-1 SNP可降低高温胁迫下生姜叶片损伤程度,维护PSII的正常生理功能,维持多胺的正常代谢,进而提高生姜植株的耐热性。 | 李秀 巩彪 王允 徐坤 | 2014 | 中国农业科学2014，47，6： | 10 |
| 11 | Blue light is more essential than red light for maintaining the activities of photosystem Ⅱ and Ⅰ and photosynthetic electron transport capacity in cucumber leaves显示文摘Blue and red lights differently regulate leaf photosynthesis. Previous studies indicated that plants under blue light generally exhibit better photosynthetic characteristics than those under red light. However, the regulation mechanism of related photosynthesis characteristics remains largely unclear. Here, four light qualities treatments(300 μmol m–2 s–1) including white fluorescent light(FL), blue monochromatic light(B, 440 nm), red monochromatic light(R, 660 nm), and a combination of red and blue light(RB, R:B=8:1) were carried out to investigate their effects on the activity of photosystem II(PSII) and photosystem I(PSI), and photosynthetic electron transport capacity in the leaves of cucumber(Cucumis sativus L.) seedlings. The results showed that compared to the FL treatment, the R treatment significantly limited electron transport rate in PSII(ETRII) and in PSI(ETRI) by 79.4 and 66.3%, respectively, increased non-light induced non-photochemical quenching in PSII(ΦNO) and limitation of donor side in PSI(ΦND) and reduced most JIP-test parameters, suggesting that the R treatment induced suboptimal activity of photosystems and inhibited electron transport from PSII donor side up to PSI. However, these suppressions were effectively alleviated by blue light addition(RB). Compared with the R treatment, the RB treatment significantly increased ETRII and ETRI by 176.9 and 127.0%, respectively, promoted photosystems activity and enhanced linear electron transport by elevating electron transport from QA to PSI. The B treatment plants exhibited normal photosystems activity and photosynthetic electron transport capacity similar to that of the FL treatment. It was concluded that blue light is more essential than red light for normal photosynthesis by mediating photosystems activity and photosynthetic electron transport capacity. | MIAO Yan-xiu W ANG Xiao-zhuo G AO Li-hong C HEN Qing-yun QU Mei | 2016 | Journal of Integrative Agriculture2016，15，1： | 9 |
| 12 | Effects of soil drought stress on photosynthetic gas exchange traits and chlorophyll fluorescence in Forsythia suspensa显示文摘To clarify the changes in plant photosynthesis and mechanisms underlying those responses to gradually increasing soil drought stress and reveal quantitative relationships between photosynthesis and soil moisture,soil water conditions were controlled in greenhouse pot experiments using 2-year-old seedlings of Forsythia suspensa(Thunb.) Vahl. Photosynthetic gas exchange and chlorophyll fluorescence variables were measured and analyzed under 13 gradients of soil water content. Net photosynthetic rate(PN), stomatal conductance(gs), and water-use efficiency(WUE) in the seedlings exhibited a clear threshold response to the relative soil water content(RSWC). The highest PNand WUEoccurred at RSWCof51.84 and 64.10%, respectively. Both PNand WUEwere higher than the average levels at 39.79% B RSWCB 73.04%. When RSWCdecreased from 51.84 to 37.52%,PN, gs, and the intercellular CO2 concentration(Ci)markedly decreased with increasing drought stress; the corresponding stomatal limitation(Ls) substantially increased, and nonphotochemical quenching(NPQ) also tended to increase, indicating that within this range of soil water content, excessive excitation energy was dispersed from photosystem II(PSII) in the form of heat, and the reduction in PNwas primarily due to stomatal limitation.While RSWCdecreased below 37.52%, there were significant decreases in the maximal quantum yield of PSII photochemistry(Fv/Fm) and the effective quantum yield of PSII photochemistry(UPSII), photochemical quenching(qP), and NPQ; in contrast, minimal fluorescence yield of the dark-adapted state(F0) increased markedly. Thus,the major limiting factor for the PNreduction changed to a nonstomatal limitation due to PSII damage. Therefore, an RSWCof 37.52% is the maximum allowable water deficit for the normal growth of seedlings of F. suspensa, and a water content lower than this level should be avoided in field soil water management. Water contents should be maintained in the range of 39.79% B RSWCB 73.04% to ensure normal function of the photosynthetic apparatus and high levels of photosynthesis and efficiency in F.suspensa. | Ying Lang Ming Wang Jiangbao Xia Qiankun Zhao | 2018 | Journal of Forestry Research2018，29，1： | 9 |
| 13 | Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance显示文摘In this study, we investigated the effects of exogenous calcium nitrate on photoinhibition and thylakoid protein level in peanut plants under heat(40 °C) and high irradiance(HI)(1,200 m mol/m2 per s) stress. Compared with control seedlings(cultivated in 0 mmol/L Ca(NO3)2medium),the maximal photochemical ef ficiency of photosystem II(PSII)in Ca2t-treated plants showed a slight decrease after 5 h stress,accompanied by lower degree of PSII closure(1-q P), higher non-photochemical quenching, and lower level of membrane damage. Ca2 t inhibitors were used to analyze the varieties of antioxidant enzymes activity and PSII proteins. These results indicated that Ca2 t could protect the subunits of PSII reaction centers from photoinhibition by reducing the generation of reactive oxygen species. In the presence of both ethyleneglycol-bis(2-aminoethylether)-tetraacetic acid and ascorbic acid(As A), the net degradation of the damaged D1 protein was faster than that only treated with As A. Our previous study showed that either the transcriptional or the translational level of calmodulin was obviously higher in Ca2t-treated plants. These results suggested that, under Researh eat and HI stress, the Ca2 t signal transduction pathway can alleviate the photoinhibition through regulating the protein repair process besides an enhanced capacity for scavenging reactive oxygen species. | Sha Yang Fang Wang Feng Guo Jing-Jing Meng Xin-Guo Li Shu-Bo Wan | 2015 | Journal of Integrative Plant Biology2015，57，5： | 7 |
| 14 | Effects of flooding stress on the photosynthetic apparatus of leaves of two Physocarpus cultivars显示文摘We applied under pot-culture conditions and the double-casing pot method to study the characteristics of photosynthetic gas exchange and chlorophyll fluorescence in the leaves of Physocarpus amurensis Maxim(PA) and Physocarpus opulifolius under flooding stress.Our results indicate a significantly higher flooding tolerance of P.opulifolius compared to P.amurensis.Especially in P.amurensis, the limitation of non-stomatal factors played a major role in the advanced stages of flooding stress,observed as a rapid increase of the intercellular CO_2 concentration(C_i) and a decrease of the stomatal limitation value(L_s).The maximal PSII photochemical efficiencies(F_v/F_m) and actual photochemical efficiency(?_(PSII)) in the leaves of P.opulifolius were significantly higher, and the extent of decrease during the flooding process was smaller than in P.amurensis.In addition, the non-chemical quenching(NPQ) in the leaves of P.opulifolius significantly increased from the 10 th day under flooding stress,while the variation of NPQ in the leaves of P.amurensis was much smaller.This indicates that the leaves of P.opulifolius had not only higher PSII photochemical activity, but also improved tolerance to flooding stress, which may be caused by its ability to dissipate excess excitation energy by starting NPQ.At the 16 th day under flooding stress, the PIABSsignificantly decreased with greater extent of decrease than F_v/F_m in the leaves of both Physocarpus,but the decreasing extent of PIABSin P.opulifolius was significantly smaller than in P.amurensis.In the 16 th day under flooding stress, the fluorescence at J and I point(V_J and V_I) in P.amurensis were significantly higher, and the extent of increase in V_J was greater than V_I.However, the variations of V_Jand V_I in the leaves of P.opulifolius were smaller, suggesting that the damage sites of flooding stress to PSII in the leaves of P.amurensis were mainly located in the electron transport process from Q_A at the PSII receptor side to Q_B.Flooding stress reduced the proportion(φ Eo) of luminous energy absorbed by the PSII reaction center for the electron transport following Q_A^-, while the maximum quantum yield(φ Do) of non-photochemical quenching increased.However, the TR_o/RC and ETo/RC in the leaves of P.amurensis decreased accompanied by a dramatic increase of energy(DI_o/RC) from the dissipation in the reaction center.This further indicated that the function of the PSII reaction center in the leaves of P.amurensis was significantly lower than in P.opulifolius. | Huihui Zhang Peng Feng Wei Yang Xin Sui Xin Li Wei Li Rongtao Zhang Siyu Gu Nan Xu | 2018 | Journal of Forestry Research2018，29，4： | 6 |
| 15 | 油桐叶成熟过程中光系统结构和功能的变化及其对SO_2的抗性显示文摘对油桐叶片成熟过程中光系统结构和功能及其对SO2的抗性进行了测定。结果表明:第4、5、6、7、8对叶片PSII光能转换效率、潜在活性和PSI光能转换效率、潜在活性最为敏感。叶片成熟过程中C18:3、UFA、IUFA和C18:3/IUFA的变化及其对SO2的抗性表明:叶4~6的变化比较稳定,即叶位4~6的叶片对于SO2的抗性比较稳定。 | 廖飞勇 何平 | 2005 | 经济林研究2005，23，1： | 6 |
| 16 | Leaf photosynthesis and yield components of mung bean under fully open-air elevated[CO_2]显示文摘Mung bean(Vigna radiata L.) has the potential to establish symbiosis with rhizobia,and symbiotic association of soil micro flora may facilitate the photosynthesis and plant growth response to elevated[CO_2].Mung bean was grown at either ambient CO_2 400 μmol mol^(-1) or[CO_2]((550+17) μmol mol^(-1)) under free air carbon dioxide enrichment(FACE) experimental facility in North China.Elevated[CO_2]increased net photosynthetic rate(P_n),water use efficiency(WUE) and the non-photochemical quenching(NPQ) of upper most fully-expanded leaves,but decreased stomatal conductance(G_s),intrinsic efficiency of PSII(F_v '/F_m'),quantum yield of PSII(φ_(PSll)) and proportion of open PSII reaction centers(q_p).At elevated[CO_2],the decrease of F_v'/F_m',φ_(PSII),q_p at the bloom stage were smaller than that at the pod stage.On the other hand,P_n was increased at elevated[CO_2]by 18.7 and 7.4%at full bloom(R2) and pod maturity stages(R4),respectively.From these findings,we concluded that as a legume despite greater nutrient supply to the carbon assimilation at elevated[CO_2],photosynthetic capacity of mung bean was still suppressed under elevated[CO_2]particularly at pod maturity stage but plant biomass and yield was increased by 11.6 and 14.2%,respectively.Further,these findings suggest that even under higher nutrient acquisition systems such as legumes,nutrient assimilation does not match carbon assimilation under elevated[CO_2]and leads photosynthesis down-regulation to elevated[CO_2]. | GAO Ji HAN Xue Saman Seneweera LI Ping ZONG Yu-zheng DONG Qi LIN Er-da HAO Xing-yu | 2015 | Journal of Integrative Agriculture2015，14，5： | 6 |
| 17 | A Mutation of OSOTP 51 Leads to Impairment of Photosystem I Complex Assembly and Serious Photo-damage in Rice显示文摘Gene expression in chloroplasts is regulated by many nuclear-encoded proteins. In this study, we isolated a rice (Oryza sativa subsp. japonica) mutant osotp51 with significant reduction in photosystem I (PSI). The osotp51 is extremely sensitive to light and accumulates a higher level of reactive oxygen species. Its leaves are almost albino when grown at 40 μmol photons/m 2 per s. However, grown at 4 μmol photons/m 2 per s, osotp51 has a similar phenotype to the wild-type. 77K chlorophyll fluorescence analysis showed a blue shift in the highest peak emission from PSI in osotp51. In addition, the level of PSI and PSII dimer is dramatically reduced in osotp51. OSOTP 51 encodes a pentatricopeptide repeats protein, homologous to organelle transcript processing 51 in Arabidopsis. Loss-of-function OSOTP51 affects intron splicing of a number of plastid genes, particularly the ycf3 coding a protein involved in the assembly of PSI complex. OSOTP51 is functionally conserved in higher plants. The mutation of osotp51 indirectly leads to a widespread change in the structure and functions of PSI, results in severe photoinhibition, and finally dies, even when grown under very low light intensity. | Jian-Wei Ye Zi-Ying Gong Chun-Guang Chen Hua-Ling Mi Gen-Yun Chen | 2012 | Journal of Integrative Plant Biology2012，54，2： | 5 |
| 18 | CO_2增施对四川弱光区设施黄瓜叶片光系统功能及其产量的影响显示文摘【目的】为探究CO_2增施对弱光区设施黄瓜叶片光系统II、光系统I、跨膜质子梯度及产量的影响。【方法】以‘川翠3号’黄瓜为材料,利用吊袋式CO_2气肥进行黄瓜的CO_2增施处理,采用分光光度计、LI-6400便携式光合测定仪和Dual-PAM-100双通道荧光测定仪分别测定处理和对照黄瓜叶片的光合色素含量、叶片气体交换参数、光系统II和光系统I荧光参数及跨膜质子梯度的550~515 nm差示信号等参数;此外,测定黄瓜叶片的叶面积、单果重、单株结果数、株高及产量等指标。【结果】CO_2增施使黄瓜叶片叶面积、叶绿素b、总叶绿素含量、表观量子效率(AQE)增高,而叶绿素a/b下降;PSII和PSI的最大电子传递速率和跨膜电位(Δψ)增高导致pmf增高。此外,CO_2增施使得黄瓜单果重、单株结果数及株高均有增加,小区产量比对照增加73.8%。由此可见,叶片接受的光能更多的用于CO_2同化作用,同时提高了黄瓜叶片的弱光利用率和抗光氧化的能力,最终通过增加单果重和单株结果数导致其产量的增加。【结论】本研究结果不仅进一步揭示了弱光区黄瓜光合系统对CO_2浓度升高的响应机制,还为四川弱光地区春季黄瓜促成栽培中应用吊袋式CO_2施肥方式提供了理论依据。 | 张泽锦 唐丽 李跃建 刘小俊 | 2018 | 西南农业学报2018，31，8： | 5 |
| 19 | LeCDJ1,a chloroplast DnaJ protein,facilitates heat tolerance in transgenic tomatoes显示文摘The roles of a tomato(Lycopersicon esculentum) chloroplast-targeted DnaJ protein(LeCDJ1) were investigated using wild-type(WT) and sense transgenic tomatoes. The LeCDJ1 expression was upregulated by 38 °C, 42 °C, 45 °C, NaCl, PEG, methyl viologen(MV) and hydrogen peroxide(H2 O2), but not by 30 °C and 35 °C. Meanwhile, LeCDJ1 was involved in the response of plants to abscisic acid(ABA). Under heat stress, the sense plants showed better growth, higher chlorophyll content, lower malondialdehyde(MDA) accumulation and relative electrical conductivity(REC), and also less PSII photoinhibition than WT. Interestingly, the sense plants treated with streptomycin(SM), an inhibitor of organellar translation, still showed higher maximum photochemistry efficiency of PSII(Fv/Fm) and D1 protein levels than the SM-untreated WT, suggesting that the protective effect of LeCDJ1 on PSII was, at least partially, independent of D1protein synthesis. Furthermore, the relatively lower superoxide radical(O2·-) and H2 O2 levels in the sense plants were considered to be due to the higher ascorbate peroxidase(APX) and superoxide dismutase(SOD) activity, which seemed unlikely dependent on their transcription level. These results indicated that LeCDJ1 overexpression facilitated heat tolerance in transgenic tomatoes. | Fanying Kong Yongsheng Deng Guodong Wang Jieru Wang Xiaoqing Liang Qingwei Meng | 2014 | Journal of Integrative Plant Biology2014，56，1： | 5 |
| 20 | Ti掺杂对a-C:H膜组织结构和性能的影响显示文摘采用磁控溅射沉积方法在钛铝合金基体上制备有Ti与无Ti的类金刚石(DLC)膜,全方位离子注入(PSII)技术被用来在膜与基体之间形成过渡层,提高膜基界面的附着力。通过X射线光电子能谱、纳米压痕、摩擦磨损等表征手段比较2种薄膜的化学组成、微结构特征和力学性能。结果表明:掺Tia-C:H膜是含有TiC纳米晶粒的复合膜结构,其中TiC晶粒的大小约是5nm,掺Tia-C:H膜的力学性能得到明显改善,硬度被提高到24GPa左右,而且结合力也明显得到提高。在大气中对样品进行热处理后,再进行摩擦磨损试验,结果表明Ti掺杂改善了a-C:H膜的热稳定性。 | 马国佳 张晓囡 武洪臣 张华芳 彭丽平 | 2008 | 稀有金属材料与工程2008，37，9： | 5 |
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