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| 1 | Jixuecao(Herba Centellae Asiaticae) alleviates mesangial cell proliferation in IgA nephropathy by inducing mitofusin 2 expression显示文摘OBJECTIVE:To investigate the effect of mitofusin 2 (Mfn2) and its downstream signaling pathway on glomerular mesangial cells (GMCs) proliferation in IgA nephropathy (IgAN),as well as the mechanism of action of Jixuecao (Herba Centellae Asiaticae,HCA) in the treatment of IgAN.METHODS:Adenovirus-mediated Mfn2 gene transfection and Mfn2 expression were analyzed by real-time polymerase chain reaction (PCR) and Western blotting.IgA1 induced the proliferation of GMCs,which were then treated with HCA.Cell proliferation was detected with cell counting kit-8 (CCK-8),and Mfn2 expression was analyzed by real-time PCR and western blotting.An IgAN animal model was also established and treated with HCA.GMCs proliferation was detected by hematoxylin-eosin staining,mitochondrial structure was analyzed by electron microscopy,mitochondrial function was determined by the Clark oxygen electrode method,and the expression of Mfn2,Phospho-extracellular regulated protein kinases1/2 (P-ERK1/2),Cyclin-dependent kinase 2 (CDK2),Phospho-p27 (p-p27),and cyclin A was analyzed by Western blotting.RESULTS:In vitro,HCA inhibited GMCs in a concentration-dependent manner in association with the upregulation of Mfn2 expression.The overexpression of Mfn2 inhibited IgA1-induced GMCs proliferation and elevated the effect of HCA.In vivo,treatment with HCA could alleviate albuminuria and creatinine and GMCs proliferation.These effects were related to the upregulation of Mfn2,p-p27 and inhibition of p-ERK1/2,CDK2,and cyclinA.Mitochondrial swelling,vacuolar degeneration,and reduction of respiratory control rate were identified in IgAN,but HCA could improve the mitochondrial structure and function.CONCLUSION:HCA inhibited GMCs proliferation via the upregulation Mfn2 and the inhibition of Ras-Raf-ERK/MAPK.We revealed that changes of mitochondrial structure and function are associated with IgAN,but that HCA can improve these mitochondrial features. | Chen Hongyu Du Yuanyuan Li Yayu Zeng Jiali Miao Jianxia Jiang Xue | 2019 | Journal of Traditional Chinese Medicine2019，39，3： | 6 |
| 2 | Valsartan Inhibits Angiotensin Ⅱ-induced Proliferation of Vascular Smooth Muscle Cells via Regulating the Expression of Mitofusin 2显示文摘Angiotensin Ⅱ (ANGⅡ) plays an important role in the pathogenesis of atherosclerosis by inducing proliferation of vascular smooth muscle cells (VSMCs).In our study,we observed the effects of valsartan on proliferation of cultured VSMCs treated with or without ANGⅡ by cell counting and methyl thiazolyl tetrazolium (MTT) assay,and detected the expression of mitofusin 2 (Mfn2),a newly discovered cell proliferation inhibitor and a related cell proliferation signaling pathway pro-tein by Western blotting.ANGⅡ at a concentration of 10-6 mol/L significantly stimulated VSMCs proliferation,down-regulated the expression of Mfn2 and upregulated the expression of Raf and ERK1/2.Valsartan inhibited such effects of ANGⅡ at concentrations of 10-5 and 10-6 mol/L,but not at 10-7 mol/L.Valsartan had no significant effect on the proliferation of untreated VSMCs.These results suggest that valsartan inhibits ANGⅡ-induced proliferation of VSMCs in vitro via Mfn2-Ras-Raf-ERK/MAPK signaling pathway. | 廖华 龚俊荣 张文娟 郭小梅 | 2012 | Journal of Huazhong University of Science and Technology(Medical Sciences)2012，32，1： | 4 |
| 3 | Mitofusin2参与石棉肺发生机制研究显示文摘[目的]以新的切入点探讨石棉引起肺泡上皮细胞凋亡的分子机制.[方法]石棉暴露下,应用免疫荧光染色免疫法和免疫印迹法观察Mitofusin 2(MFN2)的变化;分别用衣霉素(TN)、钙离子阻滞剂和MFN2RNAi处理A549细胞,观察内质网与线粒体空间距离的变化.[结果]石棉可使MFN2表达上调;与正常对照组比较,TN组、钙离子阻滞组和MFN2RNAi组内质网与线粒体间的间隙均明显缩短.[结论]MFN2在石棉肺的发生发展过程中起一定的作用. | 周煦 何惠娟 唐金凤 黄海丽 林子英 熊英环 刘刚 | 2014 | 延边大学医学学报2014，37，2： | 1 |
| 4 | 线粒体融合蛋白Mitofusin在胰岛素抵抗发生与防治中的作用显示文摘Mitofusin是线粒体融合的关键介导蛋白,与胰岛素抵抗及2型糖尿病的发生与防治密切相关。本文就Mitofusin在胰岛素抵抗发生与运动防治中的作用作一综述。 | 漆正堂 丁树哲 贺杰 | 2008 | 生命科学2008，20，4： | 1 |
| 5 | 超表达线粒体融合蛋白2基因(Mfn2)抑制小鼠卵母细胞体外成熟显示文摘线粒体融合蛋白2基因(mitofusin2,Mfn2)参与细胞凋亡和细胞周期调控,在卵母细胞和胎盘发育中起着重要作用。本研究旨在探讨Mfn2基因对小鼠(Mus musculus)卵母细胞体外成熟过程的影响。从小鼠卵巢中克隆Mfn2基因并构建p Mfn2-Venus真核表达载体。经脂质体2000介导重组质粒p Mfn2-Venus转染293T细胞(病毒包装细胞),确认重组质粒在293T细胞内的表达及定位。将p Mfn2-Venus体外转录为c RNA,并注射入小鼠卵细胞中,进行体外成熟培养,并统计卵母细胞生发泡破裂(germinalvesiclebreakdown,GVBD)发生率以及第一极体(first polarbody,PB1)排出率,荧光显微镜下观察其表达及定位。结果显示,注射了Mfn2-Venus c RNA的卵母细胞,其GVBD发生率以及PB1排出率与对照组相比显著降低(P<0.0001,P<0.05)。本研究首次揭示了Mfn2基因对小鼠卵母细胞体外成熟过程的影响,为卵母细胞体外成熟的研究提供了一个新方向,同时也为研究减数分裂过程中Mfn2基因提供了一个平台。 | 陈西锐 李建 李科瑛 许晓磊 雷安民 | 2015 | 农业生物技术学报2015，23，2： | 0 |
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