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| 1 | Structures of nucleolus and transcription sites of rRNA genes in rat liver cells显示文摘We observed the ultrastructure of nucleolus in rat liver cells by conventional electron microscopy, and employed cytochemistry NAMA-Ur DNA specific stain method to analyze the distribution and position of nucleolar DNA in situ. The results showed that nucleolar DNA of rat liver cells comes from nucleolus-associated chromatin, and continuously extends in the dense fibrillar component (DFC) of nucleolus, localizes at the periphery of fibrillar center (FC) and in DFC. Furthermore, by employing anti-DNA/RNA hybrid antibodies, we directly and selectively labeled transcription sites of rRNA genes and testified that localization of transcription sites not only to DFC but also to the periphery of FC. | 陶伟 焦明大 赫杰 何孟元 郝水 | 2000 | Science China(Life Sciences)2000，43，3： | 4 |
| 2 | Flow injection spectrophotometric determination of vitamin E in pharmaceuticals,milk powder and blood serum using potassium ferricyanide-Fe(Ⅲ) detection system显示文摘A simple and sensitive flow injection spectrophotometric method is reported for the determination of vitamin E using potassium ferricyanide-Fe(Ⅲ detection system. In the presence of vitamin E, Fe(Ⅲ/ferricyanide reduces. The in situ reduced ions are then reacted with unreduced portion of ferricyanide/Fe(Ⅲ to make soluble Prussian blue, which is monitored at absorption wavelength of 735 nm. Linear calibration graph was obtained in the concentration range of 0.1-40 μg mL-1. The relative standard deviations (n = 4) were in the range of 1.1-3.6%, with limits of detection (3 s blank) of 0.04 μg mL-1. The proposed method allowed 12 injections h-1. The method is applied to determine vitamin E in Pharmaceuticals, infant milk and blood serum samples using hexane extraction with the recoveries in the range of 93 ± 3 to 97.5 ± 4%. The method is validated using certified reference materials SRM 968c for blood serum samples. | Saima Jadoon Amir Waseem Mohammad Yaqoob Abdul Nabi | 2010 | Chinese Chemical Letters2010，21，6： | 3 |
| 3 | In-Situ Synthesis of Terbium Complex with Salicylic Acid in Silica Matrix by a Two-Step Sol-Gel Process显示文摘A process for in situ synthesis of terbium complex with salicylic acid by a two-step solgel method in silica matrix has been proposed. The luminescence properties of the silica gelscodoped with terbium and salicylic acid have also been discussed with respect to that of the geldoped with terbium and that of pure terbium complex with salicylic acid. | Lian She FU Hong Jie ZHANGl Shu Bin WANG Qing Guo MENG Km Yue YANG Jia Zuan NI(Laboratory of Rare Earth Chemistry and Physics, Changchun institute of Applied Chemistry,Chinese Academy of Sciences, Changchun, 130022) | 1998 | Chinese Chemical Letters1998，9，12： | 1 |
| 4 | Phylogenetic relationships among six species of Epistylis inferred from 185-ITS1 sequences显示文摘Phylogenetic relationships among six species of Epistylis (i. e. E. plicatilis, E. urceolata, E. chrysemydis, E. hentscheli, E. wenrichi, and E. galea) were investigated using sequences of the first internal transcribed spacer region (ITS-1) of ribosomal DNA (rDNA). Amplified rDNA fragment sequences consisted of 215 or 217 bases of the flanking 18S and 5.8S regions, and the entire ITS-1 region (from 145 to 155 bases). There were more than 33 variable bases between E. galea and the other five species in both the 18S region and the ITS-1 region. The affiliation of them was assessed using Neighbor-joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) analyses. In all the NJ, MP and ML analyses E. galea, whose macronucleic position and shape are distinctly different from those of the other five species, was probably diverged from the ancestor of Epistylis earlier than the other five species. The topology in which E. plicatilis and E. hentscheli formed a strongly supported sister clade to E. urceolata, E. chrysemydis, and E. wenrichi was consistent with variations in the thickness of the peristomial lip. We concluded that the macronucleus and peristomial lip might be the important phylogenetic characteristics within the genus Epistylis. | 缪炜 余育和 沈韫芬 张锡元 | 2002 | Science China(Life Sciences)2002，45，3： | 1 |
| 5 | The preparation of Garcinia Glycosides solid dispersion and intestinal absorption by rat in situ single pass intestinal perfusion显示文摘Garcinia Glycosides is a candidate drug obtained by structural modification of Gambogic Acid (GA), which was acquired through High Throughput Screening(HTS). As Garcinia Glycosides is an effective but insoluble anti-tumor drug, the aim of this study was to obtain a solid dispersion form Garcinia Glycosides by using solvent-melt method so that improve the solubility and dissolution rate. The solid dispersion was characterized by High Performance Liquid Chromatography (HPLC), infrared spectroscopy and evaluated the intestinal absorption of the drug by rat in situ single pass intestinal perfusion. The results showed the increase of solubility, dissolution velocity and absorption compared to other forms. This indicated that solid dispersion could greatly improve the relative bioavailability of Garcinia Glycosides in vivo. | Shengnan Li Jingchao Ji Yinghui Chen Ye Chen Ju Liu Yang Wang Hongsheng Liu | 2019 | Journal of Pharmaceutical and Biopharmaceutical Research2019，1，1： | 0 |
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