| 1 | Analysis of components of conserved 'backbone sequences' among genomes of Shigella spp. strains显示文摘Difference in the genomic compositions of prokaryotes is the basis of the diversity in their biological characters. However, besides their flora-or strain-specific genes,those floras with closer relationship in the evolution also have conserved “backbone sequences”, which reveal the marks of their origin and evolution, and these “backbone sequences”are just the basis of their elementary living abilities and common biological properties. Shigella is very closely related to E. coli in the origin and evolution, and may turn out to belong to the same genus. In this study, a microarray containing E. coli K-12 whole genome and Sf301 specific ORFs is used to investigate the genomic components of four Shigella strains. The results indicate that 16%-22% K-12 ORFs sequences are not detected in the genome of Shigella strains while the genome of Shigella contains at least 2800 conserved ORFs, which compose the common “backbone sequences”.Advanced analysis indicated that the “backbone sequences”are the essential components in maintaining the normal physiological activities of intestinal bacteria. Furthermore,only 20% Sf301-specific ORFs exist in other strains simultaneously, which demonstrate the great genome heterogeneity and the genetic diversity among the strains. | LIUHong PENGJunping YANGJian SUNLilian CHENShuxia JinQi | 2004 | Chinese Science Bulletin2004，49，2： | 5 |
| 2 | Genomic compositions and phylogenetic analysis of Shigella boydii subgroup显示文摘Comparative Genomic Hybridization (CGH) microarray analysis was used to compare the genomic compositions of all eighteen Shigella boydii serotype representative strains. The results indicated the genomic “backbone” of this subgroup contained 2552 ORFs homologous to nonpatho-genic E. coli K12. Compared with the genome of K12199 ORFs were found to be absent in all S. boydii serotype representatives, including mainly outer membrane protein genes and O-antigen bio-synthesis genes. Yet the specific ORFs of S. boydii subgroup contained basically bacteriophage genes and the function unknown (FUN) genes. Some iron metabolism, transport and type II secretion system related genes were found in most representative strains. According to the CGH phylogenetic analysis, the eighteen S. boydii serotype representatives were divided into four groups, in which se-rotype C13 strain was remarkably distinguished from the other serotype strains. This grouping result corresponded to the distribution of some metabolism related genes. Furthermore, the analysis of genome backbone genes, specific genes, and the phylogenetic trees allowed us to discover the evo-lution laws of S. boydii and to find out important clues to pathogenesis research, vaccination and the therapeutic medicine development. | WANG Jing1*, ZHANG Xiaobing1*, PENG Junping1, YANG E2, BIN Wen3, YANG Jian1, DONG Jie1, SUN Lilian1, XU Xingye1 & JIN Qi1 1. State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100176, China 2. School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China 3. School of Pharmaceutical Engineering, Shengyang Pharmaceutical University, Shengyang 110016, China | 2006 | Science China(Life Sciences)2006，49，1： | 0 |
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