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| 1 | Gene Structure and Expression of the High-affinity Nitrate Transport System in Rice Roots显示文摘Rice has a preference for uptake of ammonium over nitrate and can use ammonium-N efficiently. Consequently, transporters mediating ammonium uptake have been extensively studied, but nitrate transporters have been largely ignored. Recently, some reports have shown that rice also has high capacity to acquire nitrate from growth medium, so understanding the nitrate transport system in rice roots is very important for improving N use efficiency in rice. The present study identified four putative NRT2 and two putative NAR2 genes that encode components of the high-affinity nitrate transport system (HATS) in the rice (Oryza sativa L. subsp. japonica cv. Nipponbare) genome. OsNRT2.1 and OsNRT2.2 share an identical coding region sequence, and their deduced proteins are closely related to those from mono-cotyledonous plants. The two NAR2 proteins are closely related to those from mono-cotyledonous plants as well. However, OsNRT2.3 and OsNRT2.4 are more closely related to Arabidopsis NRT2 proteins. Relative quantitative reverse transcription-polymerase chain reaction analysis showed that all of the six genes were rapidly upregulated and then downregulated in the roots of N-starved rice plants after they were re-supplied with 0.2 mM nitrate, but the response to nitrate differed among gene members. The results from phylogenetic tree, gene structure and expression analysis implied the divergent roles for the individual members of the rice NRT2 and NAR2 families. High-affinity nitrate influx rates associated with nitrate induction in rice roots were investigated and were found to be regulated by external pH. Compared with the nitrate influx rates at pH 6.5, alkaline pH (pH 8.0) inhibited nitrate influx, and acidic pH (pH 5.0) enhanced the nitrate influx in 1 h nitrate induced roots, but did not significantly affect that in 4 to 8 h nitrate induced roots. | Chao Cai Jun-Yi Wang Yong-Guan Zhu Qi-Rong Shen Bin Li Yi-Ping Tong Zhen-Sheng Li | 2008 | Journal of Integrative Plant Biology2008，50，4： | 16 |
| 2 | 植物氮素吸收过程研究进展显示文摘氮是植株生长发育所需求的第一大矿质营养元素,主要通过根系从土壤吸收获得。因此,了解植物吸收氮素的过程及影响因素对明确氮素吸收机理,提高氮素吸收利用,促进植物生长具有重要意义。土壤中的氮素主要以无机态氮(NO-3、NH+4)和小分子有机态氮的形式存在,其中植物所能吸收利用的主要是NO-3态氮素。该研究介绍了植物对氮素的需求、NO-3吸收过程及其影响因素的研究进展。这对认识氮素吸收机理、提高氮素吸收效率具有重要的意义。 | 王平 陈举林 | 2016 | 安徽农业科学2016，44，1： | 15 |
| 3 | 低磷胁迫下不同品种水稻秧苗生长的分子生理特性显示文摘以国际上公认的化感水稻PI312777和非化感水稻Lemont(均引自美国)为材料,采用水培方法研究了不同水稻品种响应低磷(0.5mg/L,以P计)胁迫的分子生理特性.结果表明,低磷胁迫促进PI312777根的生长,对株高和干重具有微弱的抑制作用.而Lemont相应形态指标均受到较大的抑制,与PI312777相比,均达到极显著差异.低磷胁迫对PI312777相应生理生化指标的影响均低于Lemont,且达极显著水平.此外,低磷胁迫下,PI312777植株氮(N)、磷(P)、钾(K)含量均不同程度高于Lemont,具体表现为两种水稻植株N含量差异不显著,而P、K含量差异达极显著水平.N、P、K吸收利用相关的12个关键酶的基因差异表达进一步证明了前述结论.研究显示,低磷胁迫下,PI312777比Lemont具有更强的适应性能力. | 王海斌 何海斌 曾聪明 吴良展 沈荔花 熊君 林瑞余 林文雄 | 2008 | 应用与环境生物学报2008，14，5： | 12 |
| 4 | Regulation of the High-Affinity Nitrate Transport System in Wheat Roots by Exogenous Abscisic Acid and Glutamine显示文摘硝酸盐是为大多数庄稼的主要的氮(N) 来源。由根房间的硝酸盐举起是氮素代谢的一个关键步骤并且广泛地在生理、分子的层次被学习了。理解硝酸盐举起怎么被调整将帮助我们与改进硝酸盐举起效率设计庄稼。现在的学习在小麦(Triticum aestivum L.) 由外长的 abscisic 酸(骆驼毛的织物) 和夫酸安(Gln ) 调查了高亲密关系的硝酸盐运输系统(帽子) 的规定根。在作为唯一的氮包含 2 mmol/L 硝酸盐的营养液种的小麦幼苗采购原料因为 2 个星期为 4 d 被剥夺 N 并且然后为 0 被转移到当面包含 50 omol/L 骆驼毛的织物,和 1 mmol/L Gln 的营养液或 2 mmol/L 硝酸盐的缺席, 0.5 , 1 , 2 , 4 ,和 8 h 。对待的小麦植物然后被划分成二个组。一个组植物被用来调查在通过半量的 RT-PCR 的根的 NRT2 和 NAR2 基因接近的帽子部件的信使 rna 层次,并且其它植物设定被用来在包含 0.2 mmol/L 的营养液测量高亲密关系的硝酸盐流入率标记 p#eN 的硝酸盐。结果证明当硝酸盐不是在营养液的现在,但是进一步没由硝酸盐提高这些基因的正式就职时,那件外长的骆驼毛的织物在根导致了 TaNRT2.1, TaNRT2.2, TaNRT2.3, TaNAR2.1,和 TaNAR2.2 基因的表示。夫酸安,当硝酸盐在生长媒介是在场的时,被显示了禁止 NRT2 基因的表示,没禁止这正式就职。当 Gln 被供应到没有硝酸盐的营养液时,在根的这五基因的表示被导致。仅仅当硝酸盐在生长媒介是在场的时,这些结果暗示由 NRT2 表示的 Gln 的抑制发生。尽管外长的骆驼毛的织物和 Gln 在小麦的根导致了帽子基因,他们没导致硝酸盐流入。 | Chao Cai Xue-Qiang Zhao Yong-Guan Zhu Bin Li Yi-Ping Tong Zhen-Sheng Li | 2007 | Journal of Integrative Plant Biology2007，49，12： | 5 |
| 5 | 接种丛枝菌根真菌对小麦根内氮转运蛋白基因表达的影响显示文摘通过盆栽试验,研究了在低氮(不施氮)和高氮(施氮0.2 g·kg^(-1))水平下接种不同种类丛枝菌根(AM)真菌[Funneliformis mosseae(BGC-NM03D)、Claroideoglomus etunicatum(BGC-NM01B)和Rhizophagus intraradices(BJ09)]对小麦生长、氮吸收及根内4个硝态氮转运蛋白(NRT)基因、1个辅助蛋白(NAR)基因和2个铵态氮转运蛋白(AMT)基因表达的影响。结果表明,3种AM真菌均能够侵染小麦根系,以R.intraradices菌根的侵染率最高;接种R.intraradices或C.etunicatum能够显著提高小麦的生物量或地上部氮吸收量;无论是高氮还是低氮处理,接种AM真菌后均显著下调了小麦根内NRT、NAR和AMT基因的表达水平,且不同AM真菌调控小麦根内氮转运蛋白基因表达的能力具有明显差异。 | 段建锋 田慧 高亚军 | 2016 | 中国土壤与肥料2016，，2： | 5 |
| 6 | 超表达小麦硝态氮转运蛋白基因TaNRT2.1对拟南芥生长及氮吸收的影响显示文摘为了阐明小麦硝态氮转运蛋白(nitrate transporters,NRT)TaNRT2.1及辅助蛋白TaNAR2.1的硝态氮转运功能,本研究构建了TaNRT2.1单基因(单超)与TaNRT2.1+TaNAR2.1双基因超表达载体(双超),通过农杆菌介导法转化野生型拟南芥,利用潮霉素筛选与PCR鉴定分别获得了3个单超与2个双超的转基因拟南芥纯合株系。通过研究转基因拟南芥的硝态氮吸收动力学及氮含量发现:在硝态氮浓度>1 mmol·L^(-1)时,仅双超能够显著提高拟南芥的硝态氮吸收速率;硝态氮浓度<1 mmol·L^(-1)时,不论单超还是双超均不能提高拟南芥的硝态氮吸收速率。低氮(0.1 mmol·L^(-1) NO_3^-)条件下,2种转基因拟南芥的生长状况和氮吸收与野生型相比均无显著差异;而在高氮(10 mmol·L^(-1) NO_3^-)条件下,单超提高了拟南芥的角果重和植株生物量,双超则显著提高了拟南芥的生物量、根系生长和总吸氮量。这些结果表明,TaNRT2.1转运蛋白需与辅助蛋白TaNAR2.1联合才能调控拟南芥对硝态氮的转运。 | 李文虎 田汇 高亚军 | 2018 | 植物生理学报2018，54，4： | 3 |
| 7 | OsHT, a Rice Gene Encoding for a Plasma-Membrane Localized Histidine Transporter显示文摘Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OsHT (histidine transporter of Oryza sativa L.) by screening the rice cDNA library. The cDNA is 1.3kb in length and the open reading frame encodes for a 441 amino acid protein with a calculated molecular mass of 49 kDa. Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters. Computational analysis indicated that OsHT is an integral membrane protein with 11 putative transmembrane helices. This was confirmed by the transient expression assay because the OsHT-GFP fusion protein was, indeed, localized mainly in the plasma membrane of onion epidermal cells. Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae, suggesting that OsHT is a gene that encodes for a histidine transporter from rice.This is the first time that an LHT-type amino acid transporter gene has been cloned from higher plants other than A rabidopsis. | DiLIU WeiGONG YongBAI Jing-ChuLUO Yu-XianZHU | 2005 | Journal of Integrative Plant Biology2005，47，1： | 2 |
| 8 | 锌胁迫下小麦SSH文库的构建及初步分析显示文摘Zn是植物必需的微量元素,同时也是近年来造成环境污染的重金属元素之一.为了从基因表达水平揭示小麦Zn胁迫响应的分子机制,本研究利用抑制差减杂交(suppression subtractivehybridization,SSH)技术构建了Zn胁迫(0.5 mmol/L,1 mmol/L)下小麦的正反向SSH文库.从正反向文库中随机挑选阳性单克隆,并利用通用引物T7/Sp6对其进行验证.结果显示,正反库中分别获得307和821个EST序列,其片段长度在200~1 000 bp之间,它们反映了Zn胁迫下特异响应的基因.利用BLASTn和BLASTx将这些EST序列进行比对分析,在正、反库中分别筛选出221和641个uniESTs,其中751个uniESTs被注释(包括正库中193个和反库中558个).这些序列的功能主要涉及信号转导、抗氧化防御、转录与翻译、物质运输、核糖体结构、能量代谢,以及一些功能未知的基因. | 柴兴苹 张玉秀 谭金娟 王建武 陈煜娴 柴团耀 | 2013 | 中国生物化学与分子生物学报2013，29，8： | 2 |
| 9 | Effects of ozone water irrigation and spraying on physiological characteristics and gene expression of tomato seedlings显示文摘Tomato seedlings were used as experimental materials and treated with 1.0,2.0,3.0,and 4.0 mg/L ozone water irrigation and 0.2,0.4,0.6,and 0.8mg/L ozone water spray treatments.Indexes including the malondialdehyde(MDA)content,superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),activities,soil and plant analysis development(SPAD)value,and nitrogen content of leaves were measured.Furthermore,the expression of antioxidant enzyme,chlorophyll synthesis and nitrogen absorption genes was analyzed after optimal ozone water treatment.The results showed that the activities of antioxidant enzymes in tomato leaves were significantly increased,and the MDA content in tomato leaves was significantly reduced by ozone water irrigation and spray treatment,which indicated that ozone water treatment can significantly improve the stress tolerance of tomato seedlings.Ozone water irrigation and spraying could also significantly increase the leaf SPAD value and nitrogen content of tomato seedlings,and the optimal concentrations of ozone water irrigation and spraying were 3.0mg/L and 0.6 mg/L,respectively.The effect of ozone water irrigation on improving the physiological characteristics of tomato seedlings was better than that of spraying.After treatment with the optimal concentration of ozone water,the relative expression of antioxidant enzyme,chlorophyll synthesis,and nitrogen absorption genes was significantly increased,and the maximum expression level was reached at 12h.In addition,ozone water irrigation could promote the expression of genes more than ozone water spraying,which was consistent with the improvements in the physiological characteristics of the tomato seedlings. | Jin-Peng Xu Yan-Chong Yu Tao Zhang Qian Ma Hong-Bing Yang | 2021 | Horticulture Research2021，8，1： | 0 |
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