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| 1 | Association of VCAM-1 overexpression with oncogenesis,tumor angiogenesis and metastasis of gastric carcinoma显示文摘AIM: To investigate the relationship between the expression of vascular cell adhesion molecule-1 (VCAM-1) and oncogenesis,tumor angiogenesis and metastasis in gastric carcinoma,and to evaluate the clinical significance of serum VCAM-1levels in gastric cancer.METHODS: Specimens from 41 patients with gastric cancer, 8 patients with benign gastric ulcer, and 10 healthy subjects were detected for the expression of VCAM-1 by immunohistochemistry. Microvessel density (MVD) was measured by counting the endothelial cells immunostained with the monoclonal antibody CD34 at x200 magnification.Serum VCAM-1 concentrations were measured by an enzyme linked immunosorbent assay in the 41 gastric cancer patients before surgery, and at 7 days after surgery as well as in 25 healthy controls. The association between preoperative serum VCAM-1 levels and clinicopathological features, and their changes following surgery was evaluated. Tn addition, serum carcinoembryonic antigen (CEA) was also examined.RESULTS: Of the 41 gastric cancer tissues, 31 (75.6 %)were VCAM-1 positive. The VCAM-1 positive gastric cancers were more invasive and classified in the more advanced stage than the VCAM-1 negative ones. The VCAM-1 positive cancers were associated with more lymph node metastases than VCAM-1-negative ones (P<0.05). The expression of VCAM-1 was detected in tissues of two of the eight patients with gastric ulcer and two of the 10 healthy controls. The expression of VCAM-1 in gastric cancer patients was significantly more frequent than that in the healthy controls and ulcer group (both P<0.05). MVD in VCAM-1 expressing tissues was higher than that in VCAM-1 negative tissues (t=2.13,P<0.05). Serum VCAM-1 levels in gastric cancer patients were significantly higher than those in controls (t=3.4, P<0.05). There was a significant association between serum VCAM-1 levels and disease stage, as well as invasion depth of the tumor and the presence of distant metastases.The concentrations of serum CEA in gastric cancer were higher than normal controls. Both serum VCAM-1 and CEA levels decreased significantly after radical resection of the primary tumor (P<0.05). Furthermore, the serum levels of VCAM-1 were positively correlated with the expression of VCAM-1 in the tumor tissue (r=-0.85, P<0.05).CONCLUSION: The expression of VCAM-1 is closely related to oncogenesis, tumor angiogenesis and metastasis in gastric carcinoma. Serum VCAM-1 level in gastric cancer patients is significantly increased compared with normal controls, which decreases significantly after radical resection of the primary tumor. The serum concentration of VCAM-1 may be considered as an effective marker of tumor burden of gastric cancer. Moreover, overexpression of VCAM-1 in gastric cancer tissue is likely a major source of serum VCAM-1. | Yong-Bin Ding Guo-Yu Chen Jian-Guo Xia Xi-Wei Zang Hong-Yu Yang Li Yang Department of General Surgery,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,Jiangsu Province,China | 2003 | World Journal of Gastroenterology2003，9，7： | 26 |
| 2 | Inhibition of growth and metastasis of human gastric cancer implanted in nude mice by d-limonene显示文摘AIM:To investigate the effects and mechanism of d-limonene on the growth and metastasis of gastric cancer in vivo.METHODS: Metastatic model simulating human gastric cancer was established by orthotopic implantation of histologically intact human tumor tissue into gastric wall of nude mice. One percent d-limonene was orally administered at dose of 15 ml/kg every other day for seven weeks. Eight weeks after implantation, tumor weight,inhibition rate, apoptotic index (AI), microvessel density (MVD), vascular endothelial growth factor (VEGF), variation of ultrastructure, and the presence of metastasis were evaluated, respectively, after the mice were sacrificed.RESULTS: The tumor weight was significantly reduced in 5-FU group (2.55±0.28 g), d-limonene group (1.49±0.09 g)and combined treatment group (1.48±0.21 g) compared with the control group(2.73±0.23 g, P<0.05). In 5-FU group, d-limonene group, combined treatment group, the inhibition rates were 2.60%,47.58% and 46.84% and 0,respectively;AI was (3.31±0.33)%,(8.26±1.21)%,(20.99±1.84)% and (19.34±2.19)%, respectively; MVD was (8.64±2.81), (16.77±1.39), (5.32±4.26) and (5.86±2.27), respectively; VEGF expression was (45.77±4.79), (41.34±5.41),(29.71±8.92) and (28.24±8.55), respectively. The incidences of peritoneal metastasis also decreased significantly in 5-FU group(77.8%), d-limonene group(20.0%) and combined group (22.2%) compared with control group (100%) versus 62.5%,30% and 22.2%)(P<0.05). Liver metastasis was also inhibited and the incidences decreased significantly in 5-FU group, d-limonene group and combined group than that in control group (87.5% vs 55.5%, 20.0% and 22.2% respectively)(P<0.05). The incidence of ascites in control group, 5-FU group, d-limonene group and combined group was 25.0%,22.2%, 0, 0, respectively and 12.5%, 11.1% 0, 0, with respect to the metastasis rate to other organs.CONCLUSION: d-limonene has antiangiogenic and proapoptotic effects on gastric cancer, thereby inhibits tumor growth and metastasis. Combination of d-limonene with cytotoxic agents may be more effective. | Xiao-GuangLu Li-BinZhan Bing-AnFeng Ming-YangQu Li-HuaYu Ji-HongXie | 2004 | World Journal of Gastroenterology2004，10，14： | 16 |
| 3 | 中药消痰散结方抑制裸鼠原位移植人胃癌SGC-7901的生长转移显示文摘目的:研究中药消痰散结方对裸鼠原位移植人胃癌生长转移的抑制作用. 方法:建立裸鼠原位移植人胃癌模型;实验分组:对照组、中药组、化疗组、联合组各10只;观察和比较各组肿瘤抑制率和肿瘤转移情况.用免疫组化法定性分析和比较肿瘤转移相关指标P21 ras,P185,VEGF蛋白,KDR蛋白的表达,用RT-PCR法定量分析和比较各组ras,cerbB2, vEGF,KDR的表达. 结果:中药组、化疗组和联合组的抑瘤率分别为72.0%、51.3%、70.1%;在远处淋巴结转移率和脏器转移率方面, 与对照组相比,中药组、化疗组和联合组均减少了转移的发生,有显著性差异(P<0.05).中药组、化疗组、联合组在不同程度上下调了ras,cerbB2,VEGF和KDR在蛋白水平和mRNA水平的表达. 结论:消痰散结方具有抑制裸鼠原位移植入胃癌生长和转移的作用,其作用机制可能与下调了肿瘤转移相关基因ras, cerbB2,VEGF,KDR的表达有关. | 许玲 魏品康 陈亚琳 苏晓妹 秦志丰 施俊 李峻 何金 | 2004 | 世界华人消化杂志2004，12，5： | 14 |
| 4 | 奥沙利铂综合治疗胃癌的疗效及机制显示文摘目的:评价奥沙利铂(Oxaliplatin,L-OHP)对人胃癌的疗效,探讨其治疗胃癌的作用机制。方法:Ⅳ期胃癌患者22例接受包含L-OHP的联合化疗方案(L-OHP 85 mg/m^2,静脉点滴,1h,第1d;四氢叶酸钙200 mg/m^2,静脉点滴,1h,第1-5 d;5-FU 300mg/m^2,静脉注射,第1-2d,5-FU,持续静脉点滴,48h;2wk1疗程)4-6(平均4.6)个疗程.观察有效率,无进展生存时间(progression-free survival,PFS),总体生存时间及毒副作用。体外培养人中分化胃癌细胞株SGC-7901,应用MTT法检测L-OHP对细胞生长的抑制作用,并计算50%抑制浓度(IC_(50));将不同浓度梯度的L-OHP与细胞株作用后,应用流式细胞仪(ANEXIN-V标记)及TUNEL检测细胞凋亡情况。应用RT-PCR检测caspase-3m-RNA的表达。结果:有效(完全有效及部分有效)9例(40.9%)。平均PFS4.2mo,总体生存时间7.2mo。蓄积性神经毒性(全部为Ⅰ-Ⅱ级),呕吐及腹泻(1例Ⅲ级腹泻),骨髓抑制发生率分别为93.5%,20%,32.9%.浓度为1mmol/L的L—OHP与细胞株作用30min,流式细胞仪即可检测出细胞凋亡水平升高,但无统计学差异(P>0.05),1mmol/L作用2d,流式细胞仪及TUNEL均可检测到细胞凋亡水平显著性升高(P<0.05)。L-OHP作用后的细胞caspase-3 m-RNA表达升高,并与药物诱导的凋亡相关。结论:L-OHP治疗进展期胃癌安全有效.L-OHP在体外可明显抑制胃癌细胞株SGC-7901的生长,诱导细胞caspase-3m-RNA表达及凋亡。 | 林万隆 李定国 陈强 陆汉民 马小明 孙培龙 | 2003 | 世界华人消化杂志2003，11，10： | 12 |
| 5 | Clinical management of advanced gastric cancer: The role of new molecular drugs显示文摘Gastric cancer is the fourth most common malignant neoplasm and the second leading cause of death for cancer in Western countries with more than 20000 new cases yearly diagnosed in the United States.Surgery represents the main approach for this disease but,notwithstanding the advances in surgical techniques,we observed a minimal improvement in terms of overall survival with a significant increasing of relapsing disease rates.Despite the development of new drugs has significantly improved the effectiveness of chemothera-py,the prognosis of patients with unresectable or metastatic gastric adenocarcinoma remains poor.Recently,several molecular target agents have been investigated;in particular,trastuzumab represents the first target molecule showing improvements in overall survival in human epithelial growth factor 2-positive gastric cancer patients.New molecules targeting vascular epithelial growth factor,mammalian target of rapamycin,and anti hepatocyte growth factor-c-Met pathway are also under investigation,with interesting results.Anyway,it seems necessary to select more accurately the population to treat with new agents by the identification of new biomarkers in order to optimize the results.In this paper we review the actual'scenario'of targeted treatments,also focusing on the new agents in development for gastric cancer and gastro-esophageal carcinoma,discussing their efficacy and potential applications in clinical practice. | Ferdinando De Vita Natale Di Martino Alessio Fabozzi Maria Maddalena Laterza Jole Ventriglia Beatrice Savastano Angelica Petrillo Valentina Gambardella Vincenzo Sforza Luigi Marano Annamaria Auricchio Gennaro Galizia Fortunato Ciardiello Michele Orditura | 2014 | World Journal of Gastroenterology2014，20，40： | 12 |
| 6 | 胃癌组织VEGF,Flt1,bFGF,P^(53)表达与胃癌预后的关系显示文摘目的:探讨胃癌中VEGF,Fltl,bFGF,P53的表达及意义方法:应用SABC免疫组化法,研究VEGF,Fltl,bFGF, P53在胃癌中表达及与胃癌生长转移、临床病理特征、预后的关系. 结果:胃癌VEGF表达与肿瘤浸润深度有关(浆膜、浆膜外vs 肌层和黏膜层,P<0.01).P53表达与淋巴结转移相关(P53 阳性淋巴结转移组vs P53阴性淋巴结无转移组,P<0.05). VEGF与Fltl表达呈正相关(VEGF表达在Fltl阳性组vs在Fltl阴性组,P<0.01);影响胃癌预后的因素有临床病理分期、VEGF表达、肿瘤浸润深度、手术方式.Kaplan-Meier 研究显示VEGF表达与胃癌生存预后相关(P<0.05).Fltl, bFGF,P53表达与生存预后无关(P>0.05). 结论:P53表达与淋巴结转移相关.VEGF表达与肿瘤浸润深度和胃癌生存预后相关,VEGF表达可作为预测胃癌预后一项很好的指标. | 段伦喜 钟德午 胡辅珍 赵华 杨竹林 易文君 舒国顺 华颂文 | 2004 | 世界华人消化杂志2004，12，3： | 8 |
| 7 | 胃癌同证型中肿瘤转移相关基因蛋白的表达显示文摘目的:从基因蛋白表达层次探索胃癌中医证本质. 方法:从第二军医大学长征医院普外科收集术前胃癌患者, 并按中医辨证分型标准将其归属;取术后肿瘤标本,用免疫组化方法检测胃癌肿瘤中E-Cadherin、C-erbB-2、P53、nm23、ICAM-1、VEGF、KDR、MMP-2、TIMP- 29种基因蛋白表达情况. 结果:每个证型中的9个基因蛋白之间均有不同表达,统计学分析表明6证型的9个基因蛋白表达均有显著性差异P=0.0 001,进一步两两比较示同一证型内部多组基因都存在表达差异性;VEGF、E-cad、nm23表达率较高,分别为94%、90%、92%.痰湿凝结型中E-cad的蛋白表达最高,以平均秩和表示为:63.09,肝胃不和、瘀毒内阻两型E-cad表达较低. 结论:肝胃不和、瘀毒内阻两型胃癌发生转移可能主要与E-cad的缺失有关,但内部既有癌基因的高表达亦有抑癌基因高表达. | 许玲 孙大志 何金 刘龙 魏品康 | 2004 | 世界华人消化杂志2004，12，12： | 7 |
| 8 | Significance of vascular endothelial growth factor expression and its correlation with inducible nitric oxide synthase in gastric cancer显示文摘AIM:To investigate the clinical significance of the expression of VEGF165mRNA and the correlation with vascular endothelial growth factor(VEGF) protein and inducible nitric oxide synthase (iNO) in human gastric cancer.METHODS:We tested VEGF165mRNA expression in 31 cases of resected gastric cancer specimens and normal paired gastric mucosae by RT-PCR.Total RNA was extracted with TRIzol reagents,transcribed into cDNA with gligo (dT15) priming,inner controlled with β-actin expression and agarose gel isolated after PCR.VEGF expression was quantitated with IS1000 imaging system.Meanwhile we also examined expression levels of VEGF protein and iNOS in 85 cases of gastric cancer.All paraffin-embedded samples were immunohistochimically stained by streptavidin-peroxidase method (SP).RESULTS:The mean expression of VEGF165mRNA in gastric cancer was 1.125±0.356,significantly higher than that of normal paired mucosea,which was 0.760±0.278.The data indicated that the expression level of VEGF165mRNA was well related to lymph node metastasis and TNM stages of UICC.The expression levels in patients with lymph node metastasis and without lhmph node metastasis were 1.219±0.377 and 0.927±0.205 respectively (p<0.05),The expression in stages Ⅰ,Ⅱ,Ⅲ,Ⅳ was 0.934±0.194,1.262±0.386 respectively (p<0.01).Further analysis showed the lymph node metastasis rate in the group with over-expression of VEGF was higher than that in the group with low expression of VEGF(83.3% vs 46.2%),and the ratio of stage Ⅲ+Ⅳ in the group with over-expression of VEGF was also higher than that in the group with low expression with VEGF (77.8% vs 33.8%)(p<0.05).The positive rates of expression of VEGF protein and iNOS in 85 cases of gastric cancer were 75.4% and 58.8% respectively,and 50.1% of the patients showed positive staining both for iNOS and VEGF,the correlation with the two tactors was significant (p=0.018).But more intensive analysis showed the immunoreactive grades of VEGF were not associated with that of iNOS.CONCLUSIONS:The expression of VEGF165mRNA is well related with lymph node metastasis and TNM stages of UICC in gastric cancer of gastric cancer.The relationship can be observed between the expression of VEGF and iNOS in gastric cancer. | Zhen-YaSong Shu-QunWen Jia-PingPeng XuanHuang Ke-DaQian | 2004 | World Journal of Gastroenterology2004，10，9： | 6 |
| 9 | 中药复方胃康宁对人胃癌细胞VEGF及其受体表达的作用显示文摘目的:研究胃癌细胞血管内皮生长因子(VEGF)及其受体Flt、KDR的表达与中药胃康宁的调控作用. 方法:以不同剂量的中药复方胃康宁制剂给SD大鼠灌胃制备含药血清,然后以含药血清培养胃癌细胞,共同培养两代后,分别用免疫组化法和RT-PCR检测不同剂量组胃癌细胞中VEGF及其受体Fit、KDR的表达情况. 结果:与对照组(VEGF162.78±0.58,Fit:172.65±0.65) 相比,VEGF及其受体Flt在用药组胃癌细胞中的表达减弱, 染色变浅(VEGF:186.82±0.22,195.35±0.45,172.62±0.52,Fit:198.44±0.44,188.66±0.46,197.01±0.91, P<0.05),并且与用药剂量有一定的关系;VEGF mRNA及其受体Fit mRNA、KDR mRNA在用药组胃癌细胞中的表达也受到抑制. 结论:中药胃康宁对胃癌细胞血管内皮生长因子及其受体Fit、KDR的表达有抑制作用. | 闵存云 李庆明 吴伟康 | 2004 | 世界华人消化杂志2004，12，3： | 5 |
| 10 | VEGF及其受体KDR在胃癌组织新生血管中的作用显示文摘目的探讨血管内皮生长因子VEGF及其受体KDR在胃癌新生血管形成中的作用。方法应用免疫组织化学技术,检测60例胃癌组织VEGF及KDR蛋白表达和以CD105标记的微血管密度,分析VEGF、KDR和MVD及其与肿瘤大小、肿瘤分化、浸润深度、淋巴结转移、组织学分级和预后关系。结果VEGF及KDR阳性者MVD值显著高于阴性者(t=3.85,t=3.76,P<0.01)。VEGF、KDR表达和MVD与胃癌浸润深度(χ2=8.31,P<0.01;χ2=5.05,P<0.05;t=2.24,P<0.05)、淋巴结转移(χ2=10.62,P<0.01;χ2=5.66,P<0.05;t=5.19,P<0.05)、组织学分级(χ2=11.05,P<0.01;χ2=8.21,P<0.01;t=2.11,P<0.05)密切相关。VEGF及KDR表达阳性或高MVD的胃癌患者5年生存率较低(χ2=5.18,P<0.05;χ2=8.94,P<0.01;t=3.51,P<0.01)。结论VEGF及KDR与胃癌新生血管生成密切相关,对胃癌的生长和浸润转移有促进作用,VEGF及KDR和以CD105标记的MVD可作为反映胃癌生物学行为的客观指标。 | 宋鸿涛 耿敬姝 程慧 | 2006 | 实用肿瘤学杂志2006，20，1： | 5 |
| 11 | 血管内皮生长因子165基因对人胃癌细胞在体外生长及其受体表达的影响显示文摘目的:将已构建的重组腺病毒Ad-VEGF165进行扩增和纯化并观察其对人胃腺癌细胞(BGC-823)在体外生长及其受体表达的影响.方法:以人胚肾293细胞对重组腺病毒Ad-VEGF165和对照病毒Ad-GFP进行包装、扩增后感染BGC-823细胞,应用MTT比色法分析VEGF165对该种细胞体外生长的影响,并以免疫细胞化学方法检测该种细胞VEGFR-2的表达情况.结果:成功扩增及纯化了重组腺病毒Ad-VEGF165及对照病毒Ad-GFP,获得约3.2×1013pfu/L滴度的重组腺病毒和2.0×1013pfu/L滴度的对照病毒.当感染复数(MOI)为20时转导率即接近100%而无明显细胞中毒现象.MTT结果显示Ad-VEGF165组吸光度明显高于Ad-GFP组(24h:0.960±0.01vs0.737±0.01,P<0.01;48h:1.321±0.03vs0.981±0.02,P<0.01;72h:1.663±0.03vs1.207±0.01,P<0.01)及对照组(24h:0.960±0.01vs0.724±0.03,P<0.01;48h:1.321±0.03vs0.968±0.01,P<0.01;72h:1.663±0.03vs1.185±0.02,P<0.01).另外,在该细胞株上检测到了VEGFR-2的表达,且在Ad-VEGF165组其表达明显高于Ad-GFP组(62.5%vs37.6%,P<0.01)和对照组(62.5%vs34.1%,P<0.01).结论:VEGF对胃癌细胞有直接的促生长作用,并可上调其主要受体VEGFR-2的表达水平,从而进一步证实了VEGF对胃癌细胞的自分泌作用. | 关云艳 欧希龙 孙为豪 颜芳 产松苗 杨柳 | 2007 | 世界华人消化杂志2007，15，7： | 4 |
| 12 | 血管内皮生长因子及其受体KDR在疣状胃炎及胃癌组织中的表达及意义显示文摘目的:研究血管内皮生长因子(vascularendothelial growth factor,VEGF)及其受体2(kinase insert domain-containing receptor,KDR)在疣状胃炎(verrucose gastritis,VG)与胃癌中的表达的关系.方法:用免疫组织化学方法检测30例慢性浅表性胃炎、80例VG和30例胃癌组织中VEGF和KDR的表达;通过13C呼气试验判断所有入组患者有无幽门螺旋杆菌(Helicobacter pylori,H.pylori)感染.结果:VG、胃癌患者的H.pylori感染率显著高于慢性浅表性胃炎患者(2=26.4,29.697;P<0.01),而两者之间比较差异无统计学意义(2=2.229,P>0.05).VG和胃癌组织中VEGF和KDR的表达与慢性浅表性胃炎组比较差异有统计学意义(2=20.008,29.697;15.428,24.300,P<0.01);VEGF和KDR在疣状胃炎及胃癌组织中的表达差异无统计学(2=3.461,3.793;P>0.05);成熟型的疣状胃炎组织中VEGF和KDR的表达与非成熟型的疣状胃炎比较差异有统计学意义(2=6.084,10.026;P<0.05),而与胃癌组织比较差异无统计学意义(2=0.728,0.080;P>0.05);H.pylori+的疣状胃炎组织中VEGF和KDR的表达与H.pylori-的疣状胃炎组比较差异有统计学意义(2=5.637,5.398;P<0.05),而与胃癌组织比较差异无统计学意义(2=0.839,1.006;P>0.05).结论:H.pylori参与了VG的形成过程.VEGF和KDR在疣状胃炎中的表达与在胃癌中的表达相似,提示VG与胃癌之间可能存在相关性,VEGF和KDR可能参与了疣状胃炎癌变的过程.VEGF和KDR在成熟型和H.pylori+的疣状胃炎中的高表达提示成熟型的VG和H.pylori感染可能存在增加VG癌变的风险. | 陈洁 朱海杭 徐永居 顾湘 朱萍 | 2013 | 世界华人消化杂志2013，21，21： | 4 |
| 13 | 晚期胃癌靶向治疗的研究进展显示文摘胃癌是常见的致死性肿瘤,尽管在全球范围内其发病率有所下降,但在亚洲一些国家的发病率仍然很高。常规的治疗方法包括:手术、放疗、化疗,在胃癌的早期阶段起着重要作用,但对晚期胃癌(advanced gastric cancer AGC)这些治疗方法所取得的效果却是有限的。表皮生长因子受体途径和血管生成途径等逐渐成为研究晚期胃癌靶向治疗的新方向,曲妥珠单抗(Trastuzumab)对晚期胃癌有很好的疗效,目前以曲妥珠单抗为基础的化疗方案已成为HER-2+胃癌患者的一线治疗方案。因此,改善晚期胃癌的治疗效果需要对不同患者的生物学标记进行识别以了解胃癌形成过程和发现化学治疗的新分子靶向制剂。 | 石宇婷 李文新 | 2016 | 世界最新医学信息文摘2016，0，8： | 4 |
| 14 | 雌激素诱导基因PS2/TFF1在胃癌及癌前病变中的表达显示文摘目的:明确乳腺癌雌激素诱导基因PS2/TFFl在胃癌及癌前病变中的表达及其意义。方法:采用免疫组织化学方法测定121例患者(慢性浅表性胃炎20例、胃溃疡20例、不典型增生20例、不完全肠化生20例、完全肠化生21例及胃癌20例)及20名健康志愿者胃黏膜组织中PS2/TFF1蛋白的表达。结果:PS2/TFF1在慢性浅表性胃炎、胃溃疡中表达与对照组比较明显增加(慢性胃炎组 vs正常对照组P<0.01 q=7.721胃溃疡组 vs正常对照组 P<0.01 q=5.256),在不典型增生、不完全肠化生及胃癌中表达比对照组明显减低(不典型增生组 vs正常对照组P<0.01 q=8.051;不完全肠化生组 vs正常对照组P<0.01,q=11.356;胃癌组 vs正常对照组P<0.01q=8.238),在完全肠化生组PS2/TFF1表达无明显增高(20/21,95.24%),与不完全肠化生组表达(11/20,55%)相比差异显著(P<0.01 x^2=8.994)。结论:(1)PS2/TFF1蛋白在胃黏膜保护中可能起重要作用;(2)PS2/TFF1的表达缺失可能是胃肿瘤恶性转化过程中的早期事件。 | 李俊美 罗和生 姚宏昌 | 2003 | 世界华人消化杂志2003，11，9： | 3 |
| 15 | 升血颗粒对进展期胃癌患者血清血管内皮生长因子的影响显示文摘目的:观察升血颗粒对进展期胃癌患者血清血管内皮生长因子(VEGF)的影响。方法:71例患者,随机分为化疗组和中药加化疗组,观察二组疗效以及临床证候、体力状况、血清VEGF变化情况。结果:中药加化疗组治疗后肿瘤有效率高于化疗组,但差异无显著性,临床证候、体力状况改善均明显优于化疗组(均P(0.05)。中药加化疗组治疗后血清VEGF水平明显下降(P(0.01)。结论:升血颗粒治疗进展期胃癌有缩小病灶,提高生存质量,降低血清VEGF水平的作用。 | 魏爱青 张强 李兴文 | 2009 | 甘肃医药2009，28，4： | 3 |
| 16 | pAdKDR-tk自杀基因系统对鼻咽癌裸鼠肿瘤血管内皮细胞的作用显示文摘目的:研究pAdKDR-tk自杀基因系统对鼻咽癌裸鼠中肿瘤血管内皮细胞的作用。方法:建立人鼻咽癌裸鼠模型,将16只成瘤鼠随机分成AdKDR-tk/丙氧鸟苷(GCV)组(治疗组)和AdKDR-tk/PBS组(对照组),每组8只。观察治疗前后肿瘤体积变化、病理组织形态学及用免疫组织化学的方法观察肿瘤微血管密度(MVD)及血管内皮生长因子(VEGF)表达。结果:治疗组在前6d肿瘤缓慢增长,第6天后肿瘤生长受到抑制,移植瘤体积逐渐缩小,而对照组瘤体体积持续迅速增长。治疗后第6、9、14天治疗组瘤体均明显小于对照组(均P<0.01)。治疗组鼻咽癌细胞形态与对照组无明显区别,但瘤组织中有大量的细胞坏死灶;对照组MVD6.63±1.41,治疗组MVD3.50±0.93,组间差异有统计学意义(P<0.05);对照组比治疗组具有较高VEGF阳性表达,2组间VEGF表达的差异具有统计学意义。结论:AdKDR-tk/GCV系统具有抑制裸鼠鼻咽癌肿瘤血管增生及肿瘤生长的作用。 | 韩虹 邱前辉 陈少华 刘艳辉 梅平 | 2008 | 临床耳鼻咽喉头颈外科杂志2008，22，6： | 2 |
| 17 | 血管内皮生长因子反义脱氧寡核苷酶对实体瘤血管生成的抑制显示文摘 | 童允洁 刘芳 邹萍 | 2005 | 临床血液学杂志2005，18，4： | 1 |
| 18 | 胃癌细胞AGS中KDR的表达对人脐静脉内皮细胞生物学行为的影响显示文摘 目的 观察胃癌细胞AGS中血管内皮生长因子受体KDR表达水平发生变化时,其培养上清对人脐静脉内皮细胞(HUVECs)生物学行为的影响。方法 采用Westernblot及免疫细胞化学的方法检测AGS细胞中KDR的表达;利用重组腺病毒介导的KDRsiRNA转染AGS细胞,通过细胞增殖试验、迁移试验及管状结构形成试验,观察不同条件培养基作用下内皮细胞生物学特性的变化。结果 胃癌细胞AGS表达KDR,转染siRNA后表达水平降低;条件培养基影响了HUVECs增殖、迁移、形成管状结构的能力,转染siRNA的AGS细胞或亲本细胞组较RPMI1640组均增强,前者更强。结论 胃癌细胞AGS来源的条件培养基对内皮细胞的增殖、迁移、管状结构的形成具有促进作用,抑制AGS细胞中KDR的表达,这种作用被增强。 | 梁树辉 丁杰 黄大伟 潘阳林 林涛 党冬梅 时永全 吴开春 | 2005 | 现代肿瘤医学2005，13，2： | 0 |
| 19 | 反流性食管炎致食管异物并梗阻1例显示文摘 | 王涛 陈世强 | 2008 | 临床耳鼻咽喉头颈外科杂志2008，22，6： | 0 |
| 20 | 反义核苷酸技术在胃癌中的应用研究进展显示文摘 | 张晓艳 易永芬 | 2005 | 医学综述2005，11，1： | 0 |
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