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| 1 | 特异性MEK抑制剂U0126对玉米大斑病菌孢子萌发、附着胞产生和致病性的影响显示文摘目的研究MAPK信号转导途径对玉米大斑病菌生长、发育和致病性的调控作用,为明确玉米大斑病菌和玉米之间互作的分子机制奠定基础,对玉米大斑病的有效防治也有重要的理论意义。方法利用MEK特异性抑制剂U0126处理玉米大斑病菌,观测该抑制剂对玉米大斑病菌孢子萌发、附着胞发育和致病性的影响。结果U0126对玉米大斑的菌落形态和生长速度没有显著影响,可以形成正常的菌丝、分生孢子,但分生孢子萌发时间晚,芽管短,分生孢子萌发百分率和附着胞产生数目下降,玉米叶片的发病时间延迟2~3d,发病率下降30%左右。在一定浓度范围内,U0126对分生孢子萌发和附着胞产生的抑制程度随着浓度增加而上升,但随着处理时间的延长而下降。结论玉米大斑病菌的分生孢子萌发、附着胞产生和对感病玉米叶片的致病能力受U0126抑制的MAPK信号转导途径调节。 | 范永山 谷守芹 董金皋 董秉芳 | 2006 | 中国农业科学2006，39，1： | 5 |
| 2 | ERK1/2 Activities Are Dispensable for Oocyte Growth but Are Required for Meiotic Maturation and Pronuclear Formation in Mouse显示文摘Previous studies revealed that extracellular regulated kinase-1 and-2(ERK1/2) cascade plays pivotal roles in regulating oocyte meiotic cell cycle progression. However, most knowledge about the in vivo function of ERK1/2 in mammalian oocytes was indirectly obtained from analyzing the phenotypes of Mos knockout mice. In this study, we knocked out Erk1 and Erk2 in mouse oocytes as early as the primordial follicle stage using the well-characterized Gdf9-Cre mouse model, and for the first time directly investigated the in vivo function of ERK1/2 in mouse oocytes. In this novel mouse model, we observed that ERK1/2 activities in oocyte are dispensable for primordial follicle maintenance,activation and follicle growth. Different from the Mos null oocytes, the ERK1/2-deleted oocytes had well-assembled spindles at metaphase Ⅰ(MⅠ), extruded polar body-1(PB1) with normal sizes, and did not undergo a full parthenogenetic activation characterized for pronuclear formation. However, the ovulated ERK1/2-deleted oocytes had poorly-assembled metaphase Ⅱ(MⅡ) spindles, spontaneously released polar body-2(PB2), and were arrested at another metaphase called metaphase Ⅲ(MⅢ). In addition, ERK1/2 deletion prevented male pronuclear formation after fertilization, and caused female infertility. In conclusion, these results indicate that ERK1/2 activities are required for not only MⅡ-arrest maintenance, but also efficient pronuclear formation in mouse oocytes. | Yin-Li Zhang Xiao-Man Liu Shu-Yan Ji Qian-Qian Sha Jue Zhang Heng-Yu Fan | 2015 | Journal of Genetics and Genomics2015，42，9： | 4 |
| 3 | Effects of MEK-Specific Inhibitor U0126 on the Conidial Germination,Appressorium Production,and Pathogenicity of Setosphaeria turcica显示文摘Systemic studies on the effects of mitogen-activated protein kinase (MAPK) signal transduction pathway on the growthand development of Setosphaeria turcica is helpful not only in understanding the molecular mechanism of pathogen-host interaction but also in the effective control of the diseases caused by S. turcica. U0126, the specific MEK inhibitor,is used to treat S. turcica before the observation of the conidial germination, appressorium production, and pathogenicityof the pathogen. There is no significant effect of U0126 on the colony morphology and mycelium growth of the pathogen.After treatment with U0126, the growth of mycelium and conidia are normal, but the conidial germination, appressoriumproduction, and pathogenicity of S. turcica on susceptible corn leaves are significantly inhibited. Under the definiteconcentration scope, an increase in U0126 concentration increases the inhibition degree of conidial germination andappressorium production, but the inhibition degree decreases with elongation of treatment time. The conidial germination,appressorium production, and pathogenicity of S. turcica on susceptible corn leaves are regulated by the MAPK pathwayinhibited by U0126. | FAN Yong-shan GU Shou-qin DONG Jin-gao DONG Bing-fang | 2007 | Agricultural Sciences in China2007，6，1： | 4 |
| 4 | 哺乳动物细胞中一氧化氮与MAPK信号转导途径的调节关系显示文摘一氧化氮 (NO)广泛存在于各种细胞中 ,在哺乳动物的心血管系统、神经系统、免疫系统和生殖系统等正常的生理活动和病理变化中发挥着十分重要作用 .一氧化氮合酶 (NOS)催化底物后生成NO ,NO与可溶性鸟苷酸环化酶结合 ,促使GTP转化成cGMP ,再激活cGMP依赖性的蛋白激酶 (PKG) ,使蛋白质磷酸化而发挥生物学效应 .即NOS/NO/cGMP/PKG作用通路 .丝裂原活化蛋白激酶 (MAPK)是哺乳动物细胞内最重要的信号系统之一 ,在细胞增殖、生长、发育、分化、凋亡以及应激、炎症等多种生理和病理过程发挥十分重要的调节作用 ,主要是通过MAP KKK/MAPKK/MAP级联系统传递信号 .已经在多种细胞中研究发现 ,NO与MAPK信号转导途径之间有着密切的相互调节关系 .因此 ,加深对信号传导通路各个环节两者调控机理的认识 ,对某些疾病病理机制的阐明和治疗有重要理论意义和应用价值 ,因而也受到广泛的关注 .文中根据此前的报道 ,结合该实验室的研究结果 。 | 陶勇 张美佳 洪海燕 夏国良 | 2004 | 自然科学进展2004，14，12： | 3 |
| 5 | OPU卵母细胞体外成熟体系的优化研究进展显示文摘近年来,体外胚胎生产(in vitro embryo production,IVP)技术取得了很大进步,尤其是卵母细胞活体采卵(ovum pick-up,OPU)技术得到广泛应用。OPU是20世纪90年代发展起来的一项新型的能获得优秀种母牛卵母细胞的技术,该技术在动物胚胎生产和发育机理研究及人类辅助生育技术临床实践中有着广阔的应用前景。可以提高种母畜和珍贵野生动物的资源保护效率,提高优良母畜的遗传潜力,为奶牛生物技术发展提供必要的支撑技术。卵母细胞的质量是IVP系统取得成功的关键,卵母细胞的体外成熟(in vitro maturation,IVM)是成功受精和胚胎发育必不可少的步骤。卵母细胞IVM完成了卵母细胞的细胞质和核成熟,准备了发育至胚胎基因组激活所必需的所有必要成分。但体外成熟的卵母细胞发育能力低于体内成熟卵母细胞,卵母细胞减数分裂和细胞核质成熟不同步,氧化应激损伤等对卵母细胞成功受精和胚胎发育非常重要,因此,需要进一步提高卵母细胞体外成熟能力,以改善牛体外胚胎生产系统。本文从卵母细胞体外成熟面临的核质成熟不同步,氧化应激等问题进行概述,同时,叙述C型钠肽、褪黑素、FLI等物质对卵母细胞IVM的改善作用,以期为OPU卵母细胞体外成熟体系的优化提供一定的参考。 | 张培培 郝海生 杜卫华 朱化彬 李树静 余文莉 赵学明 | 2023 | 畜牧兽医学报2023，54，4： | 1 |
| 6 | 卵细胞质内单精子注射后多原核发生率对胚胎发育和妊娠结局的影响显示文摘目的:探讨多原核(pronucleus,PN)发生率对卵细胞质内单精子注射(intracytoplasmic sperm injection,ICSI)胚胎发育和妊娠结局的影响。方法:回顾性分析南京医科大学附属妇产医院生殖医学中心2016年1月—2021年12月行ICSI助孕治疗患者的临床资料。根据多PN发生率分为3组:对照组(多PN发生率=0%,n=333)、低频多PN组(0%<多PN发生率<20%,n=80)和高频多PN组(多PN发生率≥20%,n=31)。分别比较各组间胚胎发育潜能及妊娠结局情况。结果:与对照组相比,低频多PN组的获卵总数、D3可利用胚胎数增多,正常受精率降低(均P<0.017)。与对照组和低频多PN组相比,高频多PN组正常受精率、D3可利用胚胎数显著降低(均P<0.017);虽然生化妊娠率低、流产率高,但是差异无统计学意义(P>0.017)。多因素二元Logistic回归分析结果显示,与对照组相比,高频多PN组生化妊娠率(aOR=0.402,95%CI:0.186~0.870,aP=0.021)和活产率(aOR=0.247,95%CI:0.068~0.901,aP=0.034)显著降低。结论:在ICSI周期中,多PN发生率可能反映卵母细胞质量,多PN发生率≥20%预示着胚胎发育情况较差,生化妊娠率、活产率显著降低。 | 倪丹玉 杨烨 谢奇君 姜薇 凌秀凤 | 2023 | 国际生殖健康/计划生育杂志2023，42，4： | 0 |
| 7 | Regulation between nitric oxide and MAPK signal transduction in mammals显示文摘Nitric oxide (NO) is an important biological messenger in the regulation of tissue homeostasis. It exhibits a wide range of effects during physiological and pathophysiological processes. Typical beneficial properties of NO include the regulation of vascular tone, the protection of cells against apoptosis, the modulation of immune responses, and the killing of microbial pathogens. On the other hand, NO may cause severe vasodilation and myocardial depression during bacterial sepsis or act as a cytotoxic and tissue-damaging molecule in autoimmune diseases. Mitogen-activated protein kinase (MAPK) is a family of serine/threonine protein kinases that are widely distributed in mammalian cells. MAPK cascade plays pivotal roles in gene expression, cell proliferation, differentiation, neuronal survival and programmed cell death under a variety of experimental conditions. MAPKs transduce the signal for the cellular response to extracellular stresses or stimuli. The relation between them, however, has never been reviewed. Based on our researches and other reports in the field, we review their reciprocal regulatory functions. | TAOYong ZHANGMeijia HONGHaiyan XIAGuoliang | 2005 | Progress in Natural Science:Materials International2005，15，1： | 0 |
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