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| 1 | KLF4 facilitates chromatin accessibility remodeling in porcine early embryos显示文摘Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos.Current studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear.By employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos.Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts.Furthermore,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine embryos.In addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this process.Taken together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development. | Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali Xie Taotao Hu Shichun Yang Jiaqi Guan Xiaoyu Chu Juanjuan Shi Xia Zhang Falong Lu Xin Liu Yi-Liang Miao | 2024 | Science China(Life Sciences)2024，67，1： | 0 |
| 2 | 精氨酸对绵羊胴体品质、肉质特性及瘤胃细菌组成的影响显示文摘瘤胃细菌在绵羊骨骼肌脂肪酸沉积中起着重要作用。为研究精氨酸对绵羊胴体品质、肉质特性和瘤胃细菌组成的影响,探究瘤胃细菌变化与脂肪酸沉积之间的关系,利用宏基因组测序、气相色谱技术分析绵羊补饲精氨酸后瘤胃细菌组成和肌肉脂肪酸谱的变化,建立两者间的关联性。选取16只健康且体质量相近的3月龄绵羊,随机分为对照组(基础饲粮)和精氨酸组(基础饲粮+1%精氨酸),进行90 d的饲养试验。结果表明,与对照组相比,精氨酸组绵羊的背膘厚显著降低(P<0.05),背最长肌的亮度(L^(*))值、黄度(b^(*))值及剪切力值均显著减小(P<0.05),红度(a^(*))值显著增加(P<0.05)。精氨酸组背最长肌十五烷酸、十七烷酸和十七碳烯酸的含量显著低于对照组(P<0.05),二十四碳烯酸、α-亚麻酸、二十二碳六烯酸和n-3多不饱和脂肪酸的含量显著高于对照组(P<0.05);精氨酸组n-6/n-3多不饱和脂肪酸的比值显著降低(P<0.05)。宏基因组分析显示,补充精氨酸显著提高了瘤胃中梭菌属、月形单胞菌属、瘤胃球菌属和密螺旋体属的丰度(P<0.05),降低了丁酸弧菌属、副拟杆菌属、解琥珀酸菌属、甲烷短杆菌属和Butyrivibrio proteoclasticus的丰度(P<0.05)。此外,精氨酸组瘤胃中丁酸的含量显著增加(P<0.05)。关联性分析显示,丁酸弧菌属与n-3多不饱和脂肪酸呈显著负相关(P<0.05)与饱和脂肪酸呈显著正相关(P<0.05);脂肪酸含量对肉的色泽和pH值有较大的影响。综上所述,精氨酸通过降低瘤胃氢化菌的数量,减少氢化的发生,促进肉中有益脂肪酸沉积,进而改善羊肉品质。研究结果为今后通过饲粮营养干预靶向调控瘤胃细菌提高羊肉品质提供参考。 | 康乐天 王威皓 刘婷 张台武 赵欣 翟茂琴 李军 靳烨 段艳 | 2024 | 农业工程学报2024，40，1： | 0 |
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