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| 1 | Aptasensing biosynthesized phosphatidylserine with a AuNPs nanozyme-based colorimetric aptasensor显示文摘Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples.A chimeric aptamer was constructed with two homogeneous original PS aptamers.Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme,and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations.The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples.The aptasensor offers good sensitivity and selectivity,under optimal conditions,achieving monitoring and quantitation of PS in the range of 2.5-80.0μmol/L,with a limit of detection at 536.2 nmol/L.Moreover,the aptasensor provides good accuracy,with comparison rates of 98.17%-106.40%,when compared with the HPLC-ELSD.This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications. | Sai Wang Rui Ma Chengqiang Li Ling Zhang Haiyang Zhang Xuehan Li Xiangzhao Mao | 2024 | Food Science and Human Wellness2024，13，2： | 0 |
| 2 | 构建CuNPs-适配体传感器快速检测大田软海绵酸显示文摘目的 构建一种CuNPs-适配体传感器快速检测大田软海绵酸(okadaic acid, OA)。方法 基于前期研究基础,该适配体传感器基于OA适配体与OA的分子识别机制,利用适配体末端构象变化触发荧光信号变化,构建非标记荧光型CuNPs-适配体传感器,根据OA结合其适配体导致适配体末端被占据而荧光信号被抑制程度,实现对OA的高灵敏检测。同时对传感器的灵敏度、特异性、准确性等性能进行详细评价。结果 构建的CuNPs-适配体传感器的线性检测范围较广,在75.0~2400.0 nmol/L之间,检出限为1.045 nmol/L,检测贝类样本时,该传感器展现出较高的选择性和准确性。结论 构建的适配体传感器可实现对OA的高灵敏度、高特异性检测,可为其他食品靶标的简单、快速检测提供研究参考。 | 阎晓琛 李玲 亓晓燕 李娇 王赛 毛相朝 | 2023 | 食品安全质量检测学报2023，14，7： | 0 |
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