|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Efficacy evaluation and mechanism of Bacillus subtilis EBS03 against cotton Verticillium wilt显示文摘Background:In our previous study,a strain EBS03 with good biocontrol potential was screened out of 48 strains of cotton endophyte Bacillus subtilis by evaluating the controlling effect against cotton Verticillium wilt.However,its mechanism for controlling Verticillium wilt remains unclear.The objective of this study was to further clarify its con-trolling effect and mechanism against cotton Verticillium wilt.Results:The results of confrontation culture test and double buckle culture test showed that the inhibitory effects of EBS03 volatile and nonvolatile metabolite on mycelium growth of Verticillium dahliae were 70.03%and 59.00%,respectively;the inhibitory effects of sporulation and microsclerotia germination were 47.16%and 70.06%,respec-tively.In the greenhouse test,the EBS03 fermentation broth root irrigation had the highest controlling effect at 87.11%on cotton Verticillium wilt,and significantly promoted the growth of cotton seedlings.In the field experi-ment,the controlling effect of EBS03 fermentation broth to cotton Verticillium wilt was 42.54%at 60 days after cotton sowing,and the boll number per plant and boll weight in EBS03 fermentation broth seed soaking,root irrigation,and spraying treatments significantly increased by 19.48%and 7.42%,30.90%and 2.62%,15.99%and 9.20%,respec-tively.Furthermore,EBS03 improved the resistance of cotton leaves against the infection of V.dahliae,and induced the outbreak of reactive oxygen species and accumulation of callose.In addition,the results of real time fluorescent quantitative polymerase chain reaction(RT-qPCR)detection showed that EBS03 significantly induced upregulation expression level of defense-related genes PAL,POD,PPO,and PR10 in cotton leaves,enhanced cotton plant resistance to V.dahliae,and inhibited colonization level of this fungal pathogen in cotton.Conclusion:Bacillus subtilis EBS03 has a good biological defense capability,which can inhibit the growth and coloni-zation level of V.dahliae,and activate the resistance of cotton to Verticillium wilt,thus increase cotton yield. | BAI Hongyan FENG Zili ZHAO Lihong FENG Hongjie WEI Feng ZHOU Jinglong GU Aixing ZHU Heqin PENG Jun ZHANG Yalin | 2022 | Journal of Cotton Research2022，5，4： | 1 |
| 2 | 鼠李糖乳杆菌碱性磷酸酶的提取条件优化及其降解有机磷农药作用显示文摘【背景】我国作为农业大国,对农药的大量使用是不可避免的,但是农药的超范围使用、超标及高检出率对于环境的污染与人体健康的威胁日趋严重。【目的】碱性磷酸酶(alkaline phosphatase,ALP)对有机磷农药具有积极的降解作用,因此,本文对鼠李糖乳杆菌(Lactobacillus rhamnosus)Z23(LGG Z23)所产碱性磷酸酶的提取条件进行优化,并研究其对有机磷农药的降解作用。【方法】使用单因素试验和正交试验优化ALP的提取条件;使用对硝基苯酚法测定酶活力;使用分级沉淀和层析法提纯ALP;使用乙酰胆碱酯酶抑制法测定ALP对有机磷农药的降解率。【结果】LGG Z23所产ALP的最优提取条件为:细胞破碎时间15 min,破碎功率450 W,料液比(质量体积比)1:6,提取液pH 10.0,此条件下酶活力为(4.95±0.26)U/mL,比优化前提高2.11倍;对6种有机磷农药的降解率效果为敌敌畏(95.79%±0.01%)>甲基对硫磷(90.69%±0.03%)>毒死蜱(88.90%±0.02%)>敌百虫(86.07%±0.03%)>马拉硫磷(85.31%±0.02%)>乐果(83.18%±0.03%),其中对敌敌畏和甲基对硫磷的降解效果最好,可达90%以上,并且降解作用差异显著(P<0.05)。【结论】本研究为LGG Z23所产ALP的应用研究提供了理论依据和实验数据。 | 塔娜 段晓霞 何宇星 刘玮 梁程媛 张恩馨 乌云达来 | 2022 | 微生物学通报2022，49，9： | 0 |
| 3 | 基于16S rRNA分析不同肥料对辣椒根际细菌群落的影响显示文摘设施大棚土壤往往存在施用肥料种类复杂、肥料施用量过大、肥料利用率低、土壤板结等问题。为明确施用不同肥料对辣椒根际细菌群落的影响,试验采用盆栽控水法,设5个处理:未施肥土壤(CK)、添加微生物菌液态肥(A)、添加商品无机复合肥(B)、添加商品有机复合肥(C)、添加腐熟辣椒秸秆(D),并测定辣椒生长指标、土壤理化性质及微生物多样性。结果表明:B、C、D处理的辣椒生长优于CK和A处理,其中C、D处理根系活力显著高于其他处理,分别是39.99和42.37μg/(h·g)。C处理土壤有效氮、磷、钾及有机质含量最高,但在土壤孔隙度中是D处理最高,达69.85%。微生物比较中,C和D处理独有OTU个数高于CK、A、B处理。门水平中丰度排名前5的分别是放线菌Actinobacteria、变形菌Proteobacteria、绿弯菌Chloroflexi、厚壁菌Firmicutes、酸杆菌Acidobacteria。Tukey HSD分析表明,仅变形菌Proteobacteria和酸杆菌Acidobacteria有显著性差异。进一步指示物种分析表明,C处理中具有较多指示物种,如浮霉状细菌、OLB14、Sericytochromatia、Gitt-GS-136等。这些微生物在土壤有机物质周转、碳循环、腐殖质形成中发挥重要作用。环境因子分析中,有效氮和EC值与微生物关联最密切,对微生物群落影响最大,其次是有效磷和钾,最后是总孔隙度和有机质。结果表明,添加有机复合肥提高了辣椒生长质量,同时对根际土壤微生物群落有显著的影响,促进了有益微生物在土壤中的富集,改善了土壤质量。这为辣椒种植土壤生态环境改良,促进辣椒根际有益微生物的富集,优化种植技术提供了指导。 | 王灿 卢文琴 罗金超 袁恩平 张雪廷 王绍祥 | 2023 | 热带农业科学2023，43，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员