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| 1 | The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry显示文摘Flower and fruit development are two key steps for plant reproduction.The ABCE model for flower development has been well established in model plant species;however,the functions of ABCE genes in fruit crops are less understood.In this work,we identi fied an EMS mutant named R27 in woodland strawberry(Fragaria vesca),showing the conversion of petals,stamens,and carpels to sepaloid organs in a semidominant inheritance fashion.Mapping by sequencing revealed that the class E gene homolog FveSEP3(FvH4_4g23530)possessed the causative mutation in R27 due to a G to E amino acid change in the conserved MADS domain.Additional fvesep3^(CR) mutants generated by CRISPR/Cas9 displayed similar phenotypes to fvesep3-R27.Overexpressing wild-type or mutated FveSEP3 in Arabidopsis suggested that the mutation in R27 might cause a dominant-negative effect.Further analyses indicated that FveSEP3 physically interacted with each of the ABCE proteins in strawberry.Moreover,both R27 and fvesep3^(CR) mutants exhibited parthenocarpic fruit growth and delayed fruit ripening.Transcriptome analysis revealed that both common and specific differentially expressed genes were identi fied in young fruit at 6-7 days post anthesis(DPA)of fvesep3 and pollinated wild type when compared to unpollinated wild type,especially those in the auxin pathway,a key hormone regulating fruit set in strawberry.Together,we provided compelling evidence that FveSEP3 plays predominant E functions compared to other E gene homologs in flower development and that FveSEP3 represses fruit growth in the absence of pollination and promotes fruit ripening in strawberry. | Mengting Pi Shaoqiang Hu Laichao Cheng Ruhan Zhong Zhuoying Cai Zhongchi Liu Jia-Long Yao Chunying Kang | 2021 | Horticulture Research2021，8，1： | 1 |
| 2 | 草莓YABBY基因家族的鉴定及表达分析显示文摘YABBY蛋白是一类调控植物形态建成与器官发育的重要转录因子。为了研究草莓YABBY,通过生物信息学技术鉴定了森林草莓(二倍体)与栽培草莓(八倍体)的YABBY基因家族成员及其序列特征,构建了各成员与植物YABBY的系统发育树与共线性关系图,并利用RNA-seq数据与定量逆转录聚合酶链反应(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)技术分析其表达模式,构建绿色荧光蛋白(green fluorescent protein,GFP)融合表达载体,在烟草叶细胞中瞬时表达观察其亚细胞定位。研究结果表明,森林草莓有6个FvYABBY基因,栽培草莓有26个FxaYABBY基因;FvYABBY基因家族成员可归为5个不同的进化分支,与拟南芥AtYABBY基因家族存在5对直系同源。在森林草莓中,所有Fv YABBY在根和花托中基本不表达,FvYABBY1、FvYABBY2、FvYABBY5和FvYABBY6在叶、茎、花和瘦果中高表达;在栽培草莓中,FxaYABBY1、FxaYABBY2、FxaYABBY5和FxaYABBY6在瘦果中表达,所有FxaYABBY在花托中为低表达或不表达。此外,在低温、高盐和干旱等非生物胁迫下,FvYABBY1、FvYABBY3、FvYABBY4和FvYABBY6下调表达,FvYABBY5上调表达,FvYABBY2则先上调后下调表达。在烟草叶细胞中,所有FvYABBY蛋白均定位在细胞核。以上结果为草莓YABBY基因的后续功能研究奠定了基础。 | 余婷婷 沈淑容 许以灵 王欣雨 余瑶 马伯军 陈析丰 | 2024 | 生物工程学报2024，40，1： | 0 |
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