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| 1 | A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL显示文摘Bone regeneration originates from proliferation and differentiation of osteoprogenitors via either endochondral or intramembranous ossifcation;and the regeneration capacities decline with age and estrogen loss.Maillary sinus floor lifting(MSFL)is a commonly used surgical procedure for guiding bone regeneration in mailla.Radiographic analysis of 1210 clinical cases of maxilla bone regeneration after MSFL revealed that the intrasinus osteogenic eficacy was independent of age and gender,however;and this might be related to the Schneiderian membrane that lines the sinus cavity.In view of the particularity of this biological process,our present study aimed to elucidate the underlying mechanism of MSFL-induced bone regeneration.We frst established a murine model to simulate the clinical MSFL.By single cell RNA-sequencing and flow cytometry-based bulk RNA-sequencing,we identifed a novel Krt14^(+)Ctsk^(+)subset of cells that display both epithelial and mesenchymal properties and the transcriptomic feature of osteoprogenitors.Dual recombinases-mediated lineage tracing and oss-offunction analyses showed that these Krt14^(+)Ctsk^(+)progenitors contribute to both MSFL-induced osteogenesis and physiological bone homeostasis by differentiating into Krt14 Ctsk^(+)descendants which show robust osteogenic capacity.In addition,we detected a similar population of Kt14^(+)Ctsk^(+)cells in human samples of Schneiderian membrane,which show a highly similar osteogenic potential and transcriptomic feature to the corresponding cells in mice.The identifcation of this Krt14^(+)Ctsk^(+)population,featured by osteoprogenitor charateristics and dual epithelial-mesenchymal properties,provides new insight into the understanding of bone regeneration and may open more possibilities for clinical applications. | Yuteng Weng Haicheng Wang Di Wu Shuyu Xu Xiaofan Chen Jie Huang Yanhuizhi Feng Lin Li Zuolin Wang | 2022 | Cell Research2022，32，9： | 5 |
| 2 | Cre-LoxP条件性基因敲除的实际应用策略显示文摘Cre-loxP系统是起源于P1噬菌体的高效重组系统,其根据loxP位点组合不同而发生特定重组的模式使其成为近年来基因编辑最常用的工具之一。本文聚焦于Cre-loxP系统的实际应用问题,首先分析了CRISPR/Cas9系统在插入Cre序列与loxP序列中的作用与优势,随后阐述了一系列Cre-loxP系统的实际应用问题。本文阐述了Cre重组酶序列的原位插入与安全位点插入的选用、loxP序列插入的策略、Cre重组酶的标签蛋白鉴定、“异位”表达的荧光鉴定、PCR鉴定法的引物设计与工具鼠的繁殖策略等。同时,介绍了Cre-loxP系统在条件性基因敲除中的优化应用,例如配体诱导型Cre、启动子激活型Cre、光诱导型Cre与活性改造型Cre等。通过这些优化应用,可以获得对条件性基因敲除的时间可控性,也可以调节Cre重组酶的活性,甚至可以规避Cre重组酶本身的毒性。最后,论述了Cre-loxP系统面临的缺陷与挑战,展望了未来Cre-loxP系统的发展方向。总而言之,本文综述了基于Cre-loxP系统的基因敲除的实际应用,总结了目前Cre-loxP系统最前沿的研究进展和优化策略,并对未来基于Cre-loxP系统的基因编辑进行展望。本文旨在提供解决基于Cre-loxP系统实际操作问题的理论指导,并为未来更精确、更可控、更具适应性的基因编辑提供新的研究思路。 | 孔梓宇 柳毅 汪晖 | 2022 | 中国生物化学与分子生物学报2022，38，9： | 0 |
| 3 | Preface to the special topic on tissue stem cell research显示文摘Stem cells have the ability to self-renew,divide for a long period of time,and differentiate into specialized cells with distinct functions.Scientists are hopeful that,in the future,stem cells will be used to cure a wide range of diseases and traumatic injuries with some application of cell therapy.Currently,donated organs and tissues are used to replace lost or damaged tissue in many disorders. | Jinsong Li Yi Arial Zeng | 2021 | Science China(Life Sciences)2021，64，12： | 0 |
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