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| 1 | 无标记表面等离子共振免疫传感快速测定动物尿液中沙丁胺醇残留显示文摘本研究旨在建立一种无需标记的直接竞争表面等离子共振(SPR)免疫传感器,以快速测定动物尿液中沙丁胺醇的残留。试验首先利用氨基偶联原理,将沙丁胺醇单克隆抗体固定到CM7传感器芯片表面,然后通过优化单抗固定浓度、pH和固定时间,以及芯片活化和封闭时间,进而得到最佳分析条件。结果表明:沙丁胺醇在0.1~6.0 ng/mL浓度范围内线性关系良好[相关系数(R2)为0.9998],最低检出限(LOD)可达0.01 ng/mL,在添加浓度为0.5、1.0和5.0 ng/mL时,方法的平均回收率为87.82%~97.67%,批内变异系数(相对标准偏差)为1.51%~2.67%,批间变异系数为2.86%~5.53%。将该SPR免疫传感器与超高效液相色谱-串联质谱法(UPLC-MS/MS)确证分析方法进行实际尿液样品测定比较,结果能够保持一致。本研究建立的直接竞争SPR免疫传感方法简便、快速、灵敏,且无需复杂的生物标记,适用于动物尿液中沙丁胺醇残留的日常检测分析。 | 肖志明 孙铭雪 姚婷 李彦伸 黄骅 王石 索德成 樊霞 | 2023 | 动物营养学报2023，35，6： | 0 |
| 2 | Refractive Index Sensor Based on Metal-Clad Planar Polymer Waveguide Operating at 850nm显示文摘A metal-clad planar polymer waveguide refractive index sensor based on epoxy(EPO)polymer materials by using light intensity interrogation at 850 nm is designed.The polymethyl methacrylate(PMMA)material is deployed as the low refractive index(RI)buffer layer in order to better couple the optical guided mode and the surface plasmon polaritons(SPP)mode for working in water environment.The effects of the gold film thickness,PMMA buffer layer thickness,waveguide layer thickness,waveguide width,and gold length on the sensor sensing characteristics have been comprehensively studied.Simulation results demonstrate that the normalized transmission increases quasi-linearly with the increment of RI of the analyte from 1.33 to 1.46.The sensitivity is 491.5 dB/RIU,corresponding to a high RI resolution of 2.6×10^(9)RIU.The designed SPP-based optical waveguide sensor is low-cost,wide-range,and high-precision,and has a broad application prospect in biochemical sensing with merits of miniaturization,flexibility,and multiplexing. | Lanting JI Wei WEI Gang LI Shuqing YANG Yujie FU Juan SU Chi WU | 2021 | Photonic Sensors2021，11，4： | 0 |
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