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| 1 | Effects of weaning on intestinal longitudinal muscle-myenteric plexus function in piglets显示文摘Weaning piglets usually suffer from severe diarrhea(commonly known as postweaning diarrhea,PWD)along with intestinal motility disorder.Intestinal peristalsis is mainly regulated by the longitudinal muscle-myenteric plexus(LM-MP).To understand the relationship between intestinal LM-MP function and the development of PWD,we compared the intestinal electrical activity,and the transcriptional profile of the LM-MP between 21-day-old piglets(just weaned,n=7)and 24-day-old piglets(suffered the most severe weaning stress,n=7).The results showed that 24-day-old piglets exhibited different degrees of diarrhea.A significant increase in the slow-wave frequency in the ileum and colon was observed in 24-day-old piglets,while c-kit expression in the intestinal LM-MPs was significantly decreased,indicating that PWD caused by elevated slow-wave frequency may be associated with loss of c-kit.The real-time quantitative PCR(RT-q PCR)and enzyme-linked immunosorbent assay(ELISA)showed that intestinal LM-MPs in 24-day-old piglets may undergo inflammation and oxidative stress.Significant increases in 8-hydroxy-2′-deoxyguanosine and decreases in thioredoxin suggest that weaning may lead to DNA damage in the LM-MP of 24-day-old piglets.In addition,activating transcription factor 3 was significantly upregulated,indicating nerve damage in the LM-MP of 24-day-old piglets.The transcriptomic results showed that most of the differentially expressed genes in the ileal LM-MP after weaning were downregulated and closely related to the cell cycle process.Subsequent RT-q PCR analysis showed that the relative expression of p21 was upregulated,while the expression of cyclin A2,cyclin B1,and proliferating cell nuclear antigen was downregulated in the ileal and colonic LM-MP of 24-day-old piglets,suggesting that weaning may inhibit cell proliferation and cause G1/S cell cycle arrest in ileal and colonic LM-MP.In conclusion,weaning may lead to cell cycle arrest by causing DNA damage in the LM-MP,impairing intestinal motility regulation,and ultimately leading to diarrhea in piglets. | Jing Li Fenfen Liu Kaibin Mo Hengjia Ni Yulong Yin | 2024 | Science China(Life Sciences)2024，67，2： | 0 |
| 2 | 牛星状病毒和牛病毒性腹泻病毒及牛传染性鼻气管炎病毒三重荧光PCR方法的建立与应用显示文摘为快速同时检测牛星状病毒(BAstV)、牛病毒性腹泻病毒(BVDV)和牛传染性鼻气管炎病毒(IBRV),本研究针对BAstV基因组ORF1a基因、BVDV基因组多聚蛋白基因和IBRV基因组g B基因的保守区域各设计特异性引物和探针,经反应体系和扩增程序优化,建立了BAstV、BVDV和IBRV三重荧光PCR检测方法,对该方法的特异性、敏感性和重复性进行试验,并应用该方法对临床样品进行检测。结果显示,该方法可扩增BAstV、BVDV和IBRV的核酸,不能对多杀性巴氏杆菌(Pm)、B型牛鼻炎病毒(BRBV)、口蹄疫病毒(FMDV)等病原的核酸进行扩增,特异性较强;对BAstV、BVDV和IBRV的最低检测限(LOD)均为305copies/μL,敏感性较高;检测BAstV、BVDV和IBRV的组内、组间重复性试验变异系数(CV)值均小于3%,重复性较好。应用该方法检测100份临床样品,BAstV、BVDV、IBRV的阳性检出率分别为0、11%、6%。本研究建立的三重荧光PCR方法具有敏感性高、特异性强、在同一反应体系中可以快速鉴别检测BAstV、BVDV、IBRV等优点,可应用于临床BAstV、BVDV、IBRV感染的检测。 | 赵亮 温肖会 翟颀 李家奎 贾春玲 周秀蓉 王焕硕 吕殿红 罗胜军 | 2023 | 中国兽医科学2023，53，10： | 0 |
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