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| 1 | Bioreactor performance and microbial community dynamics in a production-scale biogas plant in northeastern China显示文摘In cold regions,heating is necessary to maintain the continuous and steady year-round operation of biogas fermentation.In this study,changes in the liquid composition,biogas production,and microbial diversity in heated-and unheated-phase samples were evaluated in a production-scale biogas plant that was fed continuously with cattle manure as a mono-substrate in Heilongjiang province in northeastern China.The volatile solid(VS)and volatile fatty acid(VFA)contents both gradually decreased in the heated and unheated fermentation processes.The chemical oxygen demand(COD)removal efficiency in the unheated phase sampled on June 15(s-6-15)and October 15(a-10-15)and in the heated phase sampled on January 15(w-1-15)was 63.35%,44.2%and 44.0%,respectively.The biogas production yields were in agreement with the results obtained for the VS and VFA contents and COD removal efficiency.The performance of the reactor in the heated phase was less efficient than that in the unheated phase,and the biogas production efficiency in June-August was higher than that in the other months.However,the CH4 content in the biogas remained similar all year.Moreover,ARDRA(Amplified Ribosomal DNA Restriction Analysis)was used to study the microbial community composition in the fermentation process.The results showed that the methanogenic archaeal consortium consisted mainly of members of the genera Methanomicrobiales and Methanosarcinales.In the heated phase,hydrogenotrophic methanogens represented the dominant methanogen in w-1-15 feedstock.After fermentation,the strict aceticlastic methanogen Methanosaeta became the dominant methanogen.In the unheated phase,the hydrogenotrophic methanogens and aceticlastic methanogens were equivalent in s-6-15 feedstock and effluent,and aceticlastic methanogens were dominant in both a-10-15 feedstock and effluent.Assessments of the bacteria diversity of the microbial communities revealed that the common strains in the feed and effluent of the three samples included the rumen bacteria,Bacteroides,Clostridium,Ruminococcaceae and Proteobacteria. | Gao Yamei Yang Anyi Bao Jun Ma Ruxia Yan Lei Wang Yanjie Wang Weidong | 2017 | International Journal of Agricultural and Biological Engineering2017，10，1： | 0 |
| 2 | CFD modeling and experiment of heat transfer in a tubular photo-bioreactor for photo-fermentation bio-hydrogen production显示文摘Temperature is one of the most important parameters that need to be controlled in photo-fermentation bio-hydrogen production(PFHP)system.Since the high temperature and big temperature fluctuation have adverse impacts on bio-hydrogen yield,the system numerical simulation based on the operating conditions and environmental factors is desirable.This research focused on the investigation of heat transfer properties of the PFHP system.Enzymatic hydrolysate from agricultural residues was taken as substrate,and up-flow tubular photo-bioreactor was adopted for PFHP.Temperatures inside the photo-bioreactor were monitored.The experimental design and computational modeling for the determination of the heat transfer behavior in tubular photo-bioreactor was presented.Energy balance analysis was conducted to determine the energy efficiency,and optimize the operation parameters in order to obtain higher energy efficiency.The commercial software FLUENT was also adopted in order to predict the transient temperature distribution in the photo-bioreactor.The results showed that mathematical and computational modeling method has a clear potential for improving the performance of photo-bioreactor in the process of PFHP.Up-flow tubular bioreactor has tiny temperature fluctuant,and is suitable for PFHP. | Zhang Zhiping Zhang Quanguo Yue Jianzhi Li Lianhao Zhang Tian Liu Zhengbai | 2017 | International Journal of Agricultural and Biological Engineering2017，10，1： | 0 |
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