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| 1 | The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance显示文摘DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance. | Pengxiang Chen Mingjia Yan Lei Li Jieqiang He Shuangxi Zhou Zhongxing Li Chundong Niu Chana Bao Fang Zhi Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 7 |
| 2 | 葡萄抗寒机制研究进展显示文摘葡萄在中国果树产业中占有重要地位。晚霜冻害和冬季低温冻害是制约葡萄产业持续性发展的主要因子,研究葡萄低温应答机制对培育抗寒的葡萄新品种、保证中国葡萄产业可持续发展具有重要意义。综述了国内外葡萄抗寒研究进展,从葡萄种质资源抗寒性评价、葡萄不同组织在低温下的结构变化、生理生化指标的改变、抗寒相关基因的鉴定和功能研究进展等方面介绍了所取得的成果,并对今后葡萄的抗寒育种、低温和抗抽干的发生以及不同组织器官的抗寒机制研究进行了展望。 | 张利鹏 刘怀锋 辛海平 | 2023 | 果树学报2023，40，2： | 3 |
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