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| 1 | Prunus genetics and applications after de novo genome sequencing:achievements and prospects显示文摘Prior to the availability of whole-genome sequences,our understanding of the structural and functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping of important traits and development of EST resources.With public release of the peach genome and others that followed,significant advances in our knowledge of Prunus genomes and the genetic underpinnings of important traits ensued.In this review,we highlight key achievements in Prunus genetics and breeding driven by the availability of these whole-genome sequences.Within the structural and evolutionary contexts,we summarize:(1)the current status of Prunus whole-genome sequences;(2)preliminary and ongoing work on the sequence structure and diversity of the genomes;(3)the analyses of Prunus genome evolution driven by natural and man-made selection;and(4)provide insight into haploblocking genomes as a means to define genome-scale patterns of evolution that can be leveraged for trait selection in pedigree-based Prunus tree breeding programs worldwide.Functionally,we summarize recent and ongoing work that leverages whole-genome sequences to identify and characterize genes controlling 22 agronomically important Prunus traits.These include phenology,fruit quality,allergens,disease resistance,tree architecture,and self-incompatibility.Translationally,we explore the application of sequence-based marker-assisted breeding technologies and other sequence-guided biotechnological approaches for Prunus crop improvement.Finally,we present the current status of publically available Prunus genomics and genetics data housed mainly in the Genome Database for Rosaceae(GDR)and its updated functionalities for future bioinformatics-based Prunus genetics and genomics inquiry. | Maria JoséAranzana Véronique Decroocq Elisabeth Dirlewanger Iban Eduardo Zhong Shan Gao Ksenija Gasic Amy Iezzoni Sook Jung Cameron Peace Humberto Prieto Ryutaro Tao Ignazio Verde Albert G.Abbott Pere Arús | 2019 | Horticulture Research2019，6，1： | 8 |
| 2 | 马铃薯StDRO2基因的生物信息学分析显示文摘【目的】根系构型对植物生长发育起到重要作用,DEEPER ROOTING1(DRO1)基因参与调控植物根生长角度。但是马铃薯DRO1同源基因的生物学信息还不清楚。【方法】本研究利用生物信息学软件分析了马铃薯中StDRO2蛋白的理化性质、亚细胞定位、保守结构域、系统发育树及蛋白质结构。【结果】发现马铃薯StDRO2基因编码亲水性不稳定蛋白,定位在细胞核,氨基酸序列具有IGT基因家族典型结构域。蛋白质二级结构预测显示StDRO2蛋白质均具有无规则卷曲、α-螺旋、β-转角和延伸链结构。通过实时定量PCR分析,马铃薯幼苗叶片中StDRO2的表达量显著高于根和茎杆。生长素处理后,根中StDRO2基因的表达量显著降低,说明该基因的表达受到生长素的负调控。【结论】StDRO2属于IGT基因家族中的一员,可能参与调控马铃薯根系构型。马铃薯StDRO2基因的生物信息学及表达水平检测为今后分析马铃薯根系发育提供了理论基础。 | 李葵秀 罗崇玉 傅琪景 李立池 李有春 罗浩 姚宝林 刘长宁 张红骥 于德才 杜云龙 | 2021 | 西南农业学报2021，34，4： | 1 |
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