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| 1 | Current achievements and future prospects in the genetic breeding of chrysanthemum:a review显示文摘Chrysanthemum(Chrysanthemum morifolium Ramat.)is a leading flower with applied value worldwide.Developing new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes,plant architectures,flowering times,postharvest quality,and biotic and abiotic stress tolerance in a time-and cost-efficient manner is the ultimate goal for breeders.Various breeding strategies have been employed to improve the aforementioned traits,ranging from conventional techniques,including crossbreeding and mutation breeding,to a series of molecular breeding methods,including transgenic technology,genome editing,and marker-assisted selection(MAS).In addition,the recent extensive advances in high-throughput technologies,especially genomics,transcriptomics,proteomics,metabolomics,and microbiomics,which are collectively referred to as omics platforms,have led to the collection of substantial amounts of data.Integration of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important traits.Several attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of chrysanthemum.However,applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge,primarily due to the high heterozygosity and polyploidy of the species.This review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum. | Jiangshuo Su Jiafu Jiang Fei Zhang Ye Liu Lian Ding Sumei Chen Fadi Chen | 2019 | Horticulture Research2019，6，1： | 13 |
| 2 | 茶叶CHS基因表达特性及其与儿茶素积累的关系显示文摘本研究用高效液相色谱法(HPLC)测定两种茶叶的儿茶素含量,通过RT-PCR克隆英红9号(YH)和南昆山毛叶茶(MY)的CHS基因进行比对分析,借助烟草瞬时表达技术对基因表达蛋白进行亚细胞定位;结合生物信息学分析选取特定肽段经人工合成后作为抗原制备CHS基因特异抗体;用荧光定量PCR(qRT-PCR)技术和Western blot技术测定比较两种茶叶CHS基因在转录、翻译水平的差异,并对比分析了两种茶叶间儿茶素含量差异与相应CHS基因表达的关系。结果表明:YH和MY中的儿茶素含量分别为181.51 mg/g和106.02 mg/g,前者显著高于后者并达到1.71倍;两种茶叶中所克隆的CHS基因具有单核苷酸差异但编码相同的氨基酸序列,其表达蛋白亚细胞定位于细胞质、细胞膜和细胞核;制备的CHS蛋白兔源抗体效价为1∶80000;CHS基因在YH中的表达无论在转录水平还是在翻译水平均显著高于MY,前者分别是后者的3.9倍和1.7倍,两种茶叶中CHS合成酶基因在转录、翻译水平的表达均与其对应的儿茶素积累水平一致。实验研究证明,茶叶儿茶素积累水平与其合成通路关键酶基因CHS的表达呈正相关。 | 施婧 张雪 张媛媛 林晓蓉 李斌 陈忠正 | 2020 | 食品工业科技2020，41，5： | 1 |
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