|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 葡萄重要性状基因定位及其应用显示文摘葡萄是具有重要经济价值的果树之一,其遗传育种学广受研究者关注。由于葡萄童期长、遗传杂合度高,传统杂交育种选育新品种通常耗时长、效率低。因此,通过构建葡萄遗传连锁图谱、对数量性状位点(quantitative traits locus,QTL)进行定位能够解析葡萄杂交群体中分子标记与表型之间的关系,更有针对性地选择育种亲本并对后代杂交群体进行早期鉴定,开展分子标记辅助育种(marker-assisted selection,MAS),加快育种进程。随着分子生物学和基因组测序技术的发展,自2000年开展了基因定位研究后,越来越多的QTL得到了解析。本文对近20年来发现的葡萄抗霜霉病、抗白粉病、抗根瘤蚜、浆果大小、无核、果皮颜色等重要基因的QTL定位情况进行了综述,重点介绍了葡萄色泽性状相关Myb基因定位及其在MAS中的应用,以期挖掘葡萄种质资源中与抗性、品质性状形成和调控相关的关键基因,推动葡萄定向分子设计育种进程,以及提升我国葡萄育种水平。 | 裴丹 刘众杰 葛孟清 董天宇 任艳华 房经贵 | 2022 | 南京农业大学学报2022，45，2： | 1 |
| 2 | Discovery and genome-guided mapping of REN12 from Vitis amurensis, conferring strong, rapid resistance to grapevine powdery mildew显示文摘Powdery mildew resistance genes restrict infection attempts at different stages of pathogenesis.Here,a strong and rapid powdery mildew resistance phenotype was discovered from Vitis amurensis‘PI 588631’that rapidly stopped over 97%of Erysiphe necator conidia,before or immediately after emergence of a secondary hypha from appressoria.This resistance was effective across multiple years of vineyard evaluation on leaves,stems,rachises,and fruit and against a diverse array of E.necator laboratory isolates.Using core genome rhAmpSeq markers,resistance mapped to a single dominant locus(here named REN12)on chromosome 13 near 22.8–27.0 Mb,irrespective of tissue type,explaining up to 86.9%of the phenotypic variation observed on leaves.Shotgun sequencing of recombinant vines using skim-seq technology enabled the locus to be further resolved to a 780 kb region,from 25.15 to 25.93 Mb.RNASeq analysis indicated the allele-specific expression of four resistance genes(NLRs)from the resistant parent.REN12 is one of the strongest powdery mildew resistance loci in grapevine yet documented,and the rhAmpSeq sequences presented here can be directly used for marker-assisted selection or converted to other genotyping platforms.While no virulent isolates were identified among the genetically diverse isolates and wild populations of E.necator tested here,NLR loci like REN12 are often race-specific.Thus,stacking of multiple resistance genes and minimal use of fungicides should enhance the durability of resistance and could enable a 90%reduction in fungicides in low-rainfall climates where few other pathogens attack the foliage or fruit. | Surya Sapkota Cheng Zou Craig Ledbetter Anna Underhill Qi Sun David Gadoury Lance Cadle-Davidson | 2023 | Horticulture Research2023，10，5： | 0 |
| 3 | QTL detection and candidate gene analysis of grape white rot resistance by interspecific grape (Vitis vinifera L. × Vitis davidii Foex.) crossing显示文摘Grape white rot,a devastating disease of grapevines caused by Coniella diplodiella(Speg.)Sacc.,leads to significant yield losses in grape.Breeding grape cultivars resistant to white rot is essential to reduce the regular use of chemical treatments.In recent years,Chinese grape species have gained more attention for grape breeding due to their high tolerance to various biotic and abiotic factors along with changing climatic conditions.In this study,we employed whole-genome resequencing(WGR)to genotype the parents of‘Manicure Finger’(Vitis vinifera,female)and‘0940’(Vitis davidii,male),along with 101 F1 mapping population individuals,thereby constructing a linkage genetic map.The linkage map contained 9337 single-nucleotide polymorphism(SNP)markers with an average marker distance of 0.3 cM.After 3 years of phenotypic evaluation of the progeny for white rot resistance,we confirmed one stable quantitative trait locus(QTL)for white rot resistance on chromosome 3,explaining up to 17.9%of the phenotypic variation.For this locus,we used RNA-seq to detect candidate gene expression and identified PR1 as a candidate gene involved in white rot resistance.Finally,we demonstrated that recombinant PR1 protein could inhibit the growth of C.diplodiella and that overexpression of PR1 in susceptible V.vinifera increased grape resistance to the pathogen. | Peng Li Xibei Tan Ruitao Liu Faiz Ur Rahman Jianfu Jiang Lei Sun Xiucai Fan Jihong Liu Chonghuai Liu Ying Zhang | 2023 | Horticulture Research2023，10，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员