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| 1 | 沃柑辐射诱变系渝沃无核的果实品质及遗传差异分析显示文摘【目的】从果实品质和分子水平上解析造成沃柑辐射诱变系渝沃无核重要表型差异的主要遗传因素,为后续柑橘品种遗传改良奠定基础。【方法】观测渝沃无核的果实品质,应用RNA-seq技术和全基因组重测序揭示渝沃无核的差异表达基因(differentialexpressiongenes,DEGs)及基因组的遗传差异。【结果】相比沃柑,渝沃无核的种子数量少、成熟期提前、果皮变厚和剥皮较难,其可溶性固形物含量、固酸比和抗坏血酸含量高,可滴定酸、转化糖含量低。渝沃无核的串联重复序列占基因组的3.44%,其所在位置基因的GO功能富集和KEGG通路富集主要涉及代谢过程和次生代谢物合成,其中只涉及RNA聚合酶活性、与物质的跨膜运动有关的ATPase活性、半乳糖代谢和过氧化物酶途径。DEGs的GO功能富集涉及碳水化合物代谢、氧化还原过程和β-半乳糖苷酶复合物,KEGG通路富集涉及苯丙素生物合成、内质网蛋白加工和次生代谢物合成。【结论】渝沃无核成熟期早,种子数量少,品质更优。渝沃无核与沃柑的串联重复序列的数目和位置存在差异;苯丙素生物合成、内质网蛋白加工、次生代谢物合成、糖代谢和植物激素有关途径及花粉相关DEGs存在差异。 | 彭洁 余歆 朱延松 杨胜男 程莉 李仁静 江东 | 2021 | 果树学报2021，38，12： | 3 |
| 2 | PbEIL1 acts upstream of PbCysp1 to regulate ovule senescence in seedless pear显示文摘Numerous environmental and endogenous signals control the highly orchestrated and intricate process of plant senescence.Ethylene,a well-known inducer of senescence,has long been considered a key endogenous regulator of leaf and flower senescence,but the molecular mechanism of ethylene-induced ovule senescence has not yet been elucidated.In this study,we found that blockage of fertilization caused ovule abortion in the pear cultivar‘1913’.According to transcriptome and phytohormone content data,ethylene biosynthesis was activated by pollination.At the same time,ethylene overaccumulated in ovules,where cells were sensitive to ethylene signals in the absence of fertilization.We identified a transcription factor in the ethylene signal response,ethylene-insensitive 3-like(EIL1),as a likely participant in ovule senescence.Overexpression of PbEIL1 in tomato caused precocious onset of ovule senescence.We further found that EIL1 could directly bind to the promoter of the SENESCENCE-ASSOCIATED CYSTEINE PROTEINASE 1(PbCysp1)gene and act upstream of senescence.Yeast one-hybrid and dual-luciferase assays revealed the interaction of the transcription factor and the promoter DNA sequence and demonstrated that PbEIL1 enhanced the action of PbCysp1.Collectively,our results provide new insights into how ethylene promotes the progression of unfertilized ovule senescence. | Huibin Wang Haiqi Zhang Fangfang Liang Liu Cong Linyan Song Xieyu Li Rui Zhai Chengquan Yang Zhigang Wang Fengwang Ma Lingfei Xu | 2021 | Horticulture Research2021，8，1： | 0 |
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